{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:41:52Z","timestamp":1792798912693,"version":"4.5.0"},"reference-count":79,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      A novel strain with strong antagonistic and anti-aflatoxigenic activity against\n                      <jats:italic>Aspergillus flavus<\/jats:italic>\n                      was isolated from a marine environment, highlighting its potential for biocontrol applications in food and agriculture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The strain, designated B143, was identified using a polyphasic taxonomic approach combining morphological observation and phylogenetic analysis. Its petroleum ether extract was tested for inhibitory effects on hyphal growth, spore germination, and aflatoxin B1 production in\n                      <jats:italic>A. flavus<\/jats:italic>\n                      . Transcriptome analysis was conducted to identify differentially expressed genes in aflatoxin and ergosterol biosynthesis pathways. Fluorescence staining was used to assess cell wall and membrane integrity, as well as oxidative stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Strain B143 was identified as a new species,\n                      <jats:italic>Nigrograna chlamydospora<\/jats:italic>\n                      sp. nov. Its petroleum ether extract significantly inhibited hyphal growth (70.84 \u00b1 4.29%) and spore germination (98.23 \u00b1 0.10%), and reduced aflatoxin B1 production by 35.88%. Transcriptome analysis showed down-regulation of key genes in aflatoxin B1 (adhA, 1.2-fold) and ergosterol (erg5, 1.6-fold) biosynthesis pathways. Fluorescence staining confirmed disruption of cell wall and membrane integrity and induction of oxidative stress in\n                      <jats:italic>A. flavus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate that\n                      <jats:italic>N. chlamydospora<\/jats:italic>\n                      B143 exhibits strong dual inhibitory effects against\n                      <jats:italic>A. flavus<\/jats:italic>\n                      growth and aflatoxin production, underscoring its potential as an effective biocontrol agent for mitigating contamination in food and agricultural products.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1801199","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:42:08Z","timestamp":1775022128000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Dual defense mechanisms from a novel marine fungus Nigrograna chlamydospora sp. nov. B143: antifungal and anti-aflatoxigenic properties against Aspergillus flavus"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaoyun","family":"Ou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]},{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaozhen","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shushi","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youzhi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuening","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaojie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology, College of Agriculture, Guangxi University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengfeng","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/s10600-012-0166-8","article-title":"A new spirotryprostatin from the marine isolate of the fungus Aspergillus fumigatus.","volume":"48","author":"Afiyatullov","year":"2012","journal-title":"Chem. 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