{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:10:46Z","timestamp":1779343846800,"version":"3.51.4"},"reference-count":78,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Anxiety disorders are among the most prevalent mental health conditions worldwide, and interest in psychobiotics\u2014live or inactivated microorganisms that beneficially modulate the microbiota\u2014gut\u2013brain axis\u2014is increasing. Heat killed\n                      <jats:italic>Lactobacillus brevis<\/jats:italic>\n                      SBC8803 enhances serotonin (5 hydroxytryptamine; 5 HT) signaling and ameliorates stress-related phenotypes in mammals, although the gut-brain pathways mediating these effects remain incompletely defined. Here, we investigated the anxiolytic effects and underlying molecular mechanisms of oral SBC8803 administration in adult zebrafish.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adult male AB-strain zebrafish were fed a diet containing heat killed SBC8803 for 4 weeks, and anxiety-like behavior was evaluated using the novel tank test. To explore the underlying mechanisms, we performed brain RNA sequencing and V3\u2014V4 region of 16S rRNA amplicon sequencing of intestinal contents, followed by integrative multi omics analyses, including Gene Set Variation Analysis (GSVA) combined with DIABLO-based data integration and residual correlation analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SBC8803-treated fish exhibited a shorter latency to enter the upper half of the tank and more frequent entries into this region, consistent with reduced anxiety-like behavior. Brain transcriptomic profiling identified differentially expressed genes and enrichment of serotonin receptor, CREB, and oxytocin signaling pathways, suggesting enhanced monoaminergic and plasticity-related signaling. Microbiome functional prediction indicated SBC8803-associated shifts in lipid and vitamin metabolism, including pathways related to riboflavin (vitamin B2) and tryptophan. GSVA combined with DIABLO-based data integration revealed coordinated changes between microbial metabolic and brain signaling pathways, consistent with a vitamin B\u2014serotonin\u2013anti-inflammatory axis linking gut metabolism to neural regulation. Furthermore, residual correlation analysis showed innate gut-brain coordination independent of the SBC8803 effect, such as the coupling between brain arachidonic acid and gut histidine metabolism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings support the biological validity of the SBC8803 administration-associated interactions observed in the multi-omics analyses. These findings suggest that the paraprobiotic SBC8803 may exert anxiolytic-like effects in zebrafish and reshape gut\u2014brain network states at behavioral, microbial, and transcriptomic levels, providing a potential mechanistic framework for considering heat killed SBC8803 as a candidate psychobiotic for anxiety-related conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1804536","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:43:10Z","timestamp":1779342190000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Systems-level investigation of the anxiolytic gut\u2013brain interactions induced by paraprobiotic Lactobacillus brevis SBC8803 in zebrafish"],"prefix":"10.3389","volume":"17","author":[{"given":"Azusa","family":"Kubota","sequence":"first","affiliation":[{"name":"Department of Bioinformatics and Genomics, Graduate School of Advanced Preventive Medical Sciences, Kanazawa University","place":["Kanazawa, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqing","family":"Zang","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies","place":["Mie University, Tsu, Japan"]},{"name":"Zebrafish Research Center","place":["Mie University, Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuro","family":"Shinkai","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies","place":["Mie University, Tsu, Japan"]},{"name":"Zebrafish Research Center","place":["Mie University, Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misa","family":"Nakai","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies","place":["Mie University, Tsu, Japan"]},{"name":"Zebrafish Research Center","place":["Mie University, Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Tajima","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Genomics, Graduate School of Advanced Preventive Medical Sciences, Kanazawa University","place":["Kanazawa, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhito","family":"Shimada","sequence":"additional","affiliation":[{"name":"Zebrafish Research Center","place":["Mie University, Tsu, Japan"]},{"name":"Zebra-Innovate LLC","place":["Tsu, Japan"]},{"name":"Department of Integrative Pharmacology, Graduate School of Medicine","place":["Mie University, Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1038\/s41575-023-00890-0","article-title":"Gastrointestinal and brain barriers: Unlocking gates of communication across the microbiota-gut-brain axis.","volume":"21","author":"Aburto","year":"2024","journal-title":"Nat. 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