{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:59:27Z","timestamp":1777636767722,"version":"3.51.4"},"reference-count":90,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    This study presents a comprehensive genomic and preclinical evaluation of\n                    <jats:italic>Lactococcus cremoris<\/jats:italic>\n                    FBMS_5810, establishing its taxonomic identity, genomic uniqueness, and safety profile. Genomic analyses identified strain-specific genes linked to adhesion, colonization, and pathogen exclusion, aligning with previously observed\n                    <jats:italic>in vitro<\/jats:italic>\n                    probiotic properties.\n                    <jats:italic>In vivo<\/jats:italic>\n                    studies in healthy mice demonstrated that\n                    <jats:italic>Lactococcus cremoris<\/jats:italic>\n                    FBMS_5810 modulates gut microbiota composition. Specifically, the relative abundance of Muribaculaceae, Erysipelotrichaceae, and Streptococcaceae was significantly increased in the probiotic-treated group, whereas the relative abundance of Ruminococcaceae, Bacteroidaceae, Porphyromonadaceae, and Dehalobacteriaceae was decreased. Administration of\n                    <jats:italic>Lactococcus cremoris<\/jats:italic>\n                    FBMS_5810 was also associated with changes in intestinal gene expression: in the ileum,\n                    <jats:italic>Tnf<\/jats:italic>\n                    and\n                    <jats:italic>Il1b<\/jats:italic>\n                    expression increased, while in the cecum,\n                    <jats:italic>Zo1<\/jats:italic>\n                    expression was elevated. These findings may indicate a role in supporting intestinal homeostasis and could be linked to reduced susceptibility to diet- and inflammation-related disorders. Overall, these results suggest that\n                    <jats:italic>Lactococcus cremoris<\/jats:italic>\n                    FBMS_5810 may be a useful candidate for further investigation in the development of health-oriented microbial products. By integrating genomic characterization with preclinical evaluation, this study not only highlights\n                    <jats:italic>Lactococcus cremoris<\/jats:italic>\n                    FBMS_5810 as a promising candidate but also provides a systematic approach for the identification and validation of probiotics, advancing both fundamental understanding and translational applications in molecular microbiology.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1812433","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:54:19Z","timestamp":1777442059000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization and preclinical evaluation of the candidate probiotic strain Lactococcus cremoris FBMS_5810"],"prefix":"10.3389","volume":"17","author":[{"given":"Ioanna","family":"Farmakioti","sequence":"first","affiliation":[{"name":"Department of Molecular 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