{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T06:18:44Z","timestamp":1783491524292,"version":"3.55.0"},"reference-count":86,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Several strains of\n                    <jats:italic>Kocuria rhizophila<\/jats:italic>\n                    have been reported to tolerate ionizing radiation and other environmental stresses; however, this phenotype is unevenly distributed across the species. Here, we characterize\n                    <jats:italic>K. rhizophila<\/jats:italic>\n                    strain 301, isolated from chronically radioactive radon springs in J\u00e1chymov (Czech Republic). Strain 301 displayed exceptional stress resilience, retaining approximately 10% viability after exposure to 1.0 kGy of \u03b3-irradiation, and maintaining ~20% survival following 30 days of desiccation. These responses sharply contrasted with the pronounced sensitivity of the type strain\n                    <jats:italic>K. rhizophila<\/jats:italic>\n                    TA68. Complete genome sequencing produced a single circular chromosome of 2.77 Mbp. Comparative genomic analyses revealed extensive duplication of genes involved in DNA repair, antioxidant defense, and metal ion homeostasis, including multiple paralogs of\n                    <jats:italic>uvrA, uvrD, sodA<\/jats:italic>\n                    , and Mn\/Fe transport systems. In contrast to the paradigm established by\n                    <jats:italic>Deinococcus radiodurans<\/jats:italic>\n                    , strain 301 lacks radiation-specific or lineage-exclusive genes, instead suggesting that resilience may be associated with quantitative reinforcement of conserved cellular pathways. Pan-genome analysis further demonstrated a closed\n                    <jats:italic>K. rhizophila<\/jats:italic>\n                    pangenome, with strain 301 forming a distinct phylogenetic lineage.\n                  <\/jats:p>\n                  <jats:p>\n                    Together, these findings position\n                    <jats:italic>K. rhizophila<\/jats:italic>\n                    301 as a model system of adaptation to chronic radiation exposure and illustrate how sustained environmental pressure may promote modifications and adaptations based on core functions rather than novel innovative genetic traits.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1814458","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:57:06Z","timestamp":1783490226000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic signatures of radiation stress adaptations in Kocuria rhizophila: insights from strain 301 of the J\u00e1chymov radon springs"],"prefix":"10.3389","volume":"17","author":[{"given":"Elizaveta","family":"Timkina","sequence":"first","affiliation":[{"name":"Department of Biotechnology, University of Chemistry and Technology","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Palyzov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Mare\u0161ov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sof\u00eda G.","family":"Zavala-Meneses","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Mat\u0301\u00e1tkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Chemistry and Technology","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Jaro\u0161ov\u00e1 Kolouchov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Chemistry and Technology","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1089\/153110702762027844","article-title":"A culture-independent survey of the bacterial community in a radon hot spring","volume":"2","author":"Anitori","year":"2002","journal-title":"Astrobiology"},{"key":"B2","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.jenvrad.2012.04.009","article-title":"Radio-resistance in psychrotrophic Kocuria sp. 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