{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T07:02:26Z","timestamp":1779778946558,"version":"3.53.1"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The pathogen-specific lytic activity of bacteriophages has rendered them a highly promising antibacterial agent to control\n                      <jats:italic>Salmonella<\/jats:italic>\n                      , a critical foodborne pathogen with increasing antibiotic resistance. However, the issue of phage-resistant strains severely hindered phage applications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study isolated a broad-spectrum bacteriophage JN06 from wastewater. The morphology\/host range, infection capacity\/stability and genome characteristics of JN06 were determined. Phage JN06 was evaluated in both biofilm inhibition and lettuce decontamination, followed by inoculation with\n                      <jats:italic>Salmonella<\/jats:italic>\n                      . The receptor for phage JN06 was identified by comparing adsorption abilities in resistant derivatives as well as mutants of gene deletion with\n                      <jats:italic>S. enteritidis<\/jats:italic>\n                      SMT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      JN06 (classified into\n                      <jats:italic>Jerseyvirus<\/jats:italic>\n                      based on nucleotide homology) can lyse\n                      <jats:italic>Salmonella<\/jats:italic>\n                      strains of various serotypes, with a short latent period (6 min), a large burst size (131 PFU\/infected cell), and high stability at different temperatures (4 \u00b0C-70 \u00b0C) and pH (2\u201312). Furthermore, no determinants associated with lysogenicity, virulence, or antibiotic resistance were detected in its genome. Notably, it exhibited a biofilm formation inhibition rate of 86.58%, and completely controlled\n                      <jats:italic>Salmonella<\/jats:italic>\n                      from artificially contaminated lettuce. O-antigen was identified as the likely adsorption recipient, and molecular docking revealed that the specific binding between its tail fiber protein and the main chain of Gal-initiated O-antigen, D-Man\n                      <jats:italic>p<\/jats:italic>\n                      -(1\u202f\u2192\u202f4)-L-Rha\n                      <jats:italic>p<\/jats:italic>\n                      -(\u03b11\u202f\u2192\u202f3)-D-Gal\n                      <jats:italic>p<\/jats:italic>\n                      , underlying JN06\u2019s lytic activity against multiple serotypes of\n                      <jats:italic>Salmonella<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Collectively, phage JN06 is a highly promising antibacterial agent for controlling Salmonella, benefiting food processing and disease prevention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1827076","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T06:04:12Z","timestamp":1779775452000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and characterization of a broad-spectrum Salmonella phage targeting featural foodborne serotypes"],"prefix":"10.3389","volume":"17","author":[{"given":"Ling","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jiangxi Agricultural University","place":["Nanchang, China"]},{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Shu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jiangxi Agricultural University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1038\/s41586-020-2007-4","article-title":"Clades of huge phages from across earth's ecosystems","volume":"578","author":"Al-Shayeb","year":"2020","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e02173","DOI":"10.1128\/JVI.02173-20","article-title":"Decoding the molecular properties of mycobacteriophage d29 holin provides insights into holin engineering","volume":"95","author":"Bavda","year":"2021","journal-title":"J. Virol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/j.lwt.2016.11.073","article-title":"Ready-to-eat vegetables: current problems and potential solutions to reduce microbial risk in the production chain","volume":"85","author":"Castro-Ib\u00e1\u00f1ez","year":"2017","journal-title":"LWT Food Sci. Technol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"110848","DOI":"10.1016\/j.foodcont.2024.110848","article-title":"Isolation and characterization of a novel phage ST BD for the biological control of Salmonella in dairy food matrices","volume":"168","author":"Chahar","year":"2025","journal-title":"Food Control"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1186\/s12866-025-03803-0","article-title":"Two virulent Vibrio campbellii phages with potential for phage therapy in aquaculture","volume":"25","author":"Ding","year":"2025","journal-title":"BMC Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"2451048","DOI":"10.1080\/22221751.2025.2451048","article-title":"Exploration of the feasibility of clinical application of phage treatment for multidrug-resistant Serratia marcescens-induced pulmonary infection","volume":"14","author":"Duan","year":"2025","journal-title":"Emerg Microbes Infect."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"115638","DOI":"10.1016\/j.lwt.2023.115638","article-title":"Isolation, characterization, and preliminary application of three vibrio phages in controlling Vibrio alginolyticus","volume":"191","author":"Gao","year":"2024","journal-title":"LWT"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"W181","DOI":"10.1093\/nar\/gkn179","article-title":"The CGview server: a comparative genomics tool for circular genomes","volume":"36","author":"Grant","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"Quast: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1038\/s41467-019-08853-3","article-title":"Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage","volume":"10","author":"Hendriksen","year":"2019","journal-title":"Nat. Commun."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1017\/S0950268815000047","article-title":"Outbreaks attributed to fresh leafy vegetables, United States, 1973-2012","volume":"143","author":"Herman","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"37956","DOI":"10.1038\/srep37956","article-title":"Quorum regulated resistance of Vibrio cholerae against environmental bacteriophages","volume":"6","author":"Hoque","year":"2016","journal-title":"Sci. Rep."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1073\/pnas.1617415113","article-title":"Quorum sensing controls the Pseudomonas aeruginosa crispr-cas adaptive immune system","volume":"114","author":"Hoyland-Kroghsbo","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1391777","DOI":"10.3389\/fmicb.2024.1391777","article-title":"Characterization of Salmonella phage of the genus kayfunavirus isolated from sewage infecting clinical strains of Salmonella enterica","volume":"15","author":"Juliet","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"15696","DOI":"10.1074\/jbc.M112.354837","article-title":"Structural characterization of closely related O-antigen lipopolysaccharide (LPS) chain length regulators","volume":"287","author":"Kalynych","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"4963","DOI":"10.1128\/jb.00339-11","article-title":"Identification of the lipopolysaccharide core of Yersinia pestis and Yersinia pseudotuberculosis as the receptor for bacteriophage \u03c6a1122","volume":"193","author":"Kiljunen","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1747-0765.2007.00197.x","article-title":"Ecology of viruses in soils: past, present and future perspectives","volume":"54","author":"Kimura","year":"2008","journal-title":"Soil Sci. Plant Nutr."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1111\/mmi.12933","article-title":"A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the o-antigen of the lipopolysaccharide","volume":"96","author":"Kintz","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e68562","DOI":"10.1371\/journal.pone.0068562","article-title":"Mapping the tail fiber as the receptor binding protein responsible for differential host specificity of Pseudomonas aeruginosa bacteriophages PaP1 and JG004","volume":"8","author":"Le","year":"2013","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"4756","DOI":"10.1111\/ijfs.15070","article-title":"A bacteriophage JN02 infecting multidrug-resistant Shiga toxin-producing Escherichia coli: isolation, characterisation and application as a biocontrol agent in foods","volume":"56","author":"Li","year":"2021","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1601.e1","DOI":"10.1016\/j.cmi.2023.08.022","article-title":"Development of phage resistance in multidrug-resistant Klebsiella pneumoniae is associated with reduced virulence: a case report of a personalised phage therapy","volume":"29","author":"Li","year":"2023","journal-title":"Clin. Microbiol. 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Bioelectron."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"427","DOI":"10.3390\/pharmaceutics14020427","article-title":"Phages against pathogenic bacterial biofilms and biofilm-based infections: a review","volume":"14","author":"Liu","year":"2022","journal-title":"Pharmaceutics"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"115852","DOI":"10.1016\/j.foodres.2025.115852","article-title":"Isolation and characterization of Salmonella Typhimurium monophasic variant phage and its application in foods","volume":"203","author":"Liu","year":"2025","journal-title":"Food Res. Int."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"165203","DOI":"10.1016\/j.scitotenv.2023.165203","article-title":"Genomic epidemiology of mcr carrying multidrug-resistant ST34 Salmonella enterica serovar Typhimurium in a one health context: the evolution of a global menace","volume":"896","author":"Luo","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"108831","DOI":"10.1016\/j.ijfoodmicro.2020.108831","article-title":"Prediction of Salmonella serovars isolated from clinical and food matrices in Lebanon and genomic-based investigation focusing on enteritidis serovar","volume":"333","author":"Mansour","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"96","DOI":"10.3201\/eid3001.221450","article-title":"Predictive mapping of antimicrobial resistance for Escherichia coli, Salmonella, and Campylobacter in food-producing animals, europe, 2000-2021","volume":"30","author":"Mulchandani","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"100223","DOI":"10.1016\/j.egg.2024.100223","article-title":"Whole genome sequencing of bacteriophage NINP13076 isolated against Salmonella enteritidis","volume":"30","author":"Naveen Kumar","year":"2024","journal-title":"Ecol. Genet. Genomics"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"17785","DOI":"10.1038\/s41598-022-22784-y","article-title":"Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus","volume":"12","author":"Ongenae","year":"2022","journal-title":"Sci. Rep.-Uk"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"115184","DOI":"10.1016\/j.lwt.2023.115184","article-title":"Isolation and characterization of two lytic phages against multidrug-resistant Salmonella and their application as a cocktail for biocontrol in foods","volume":"185","author":"Pan","year":"2023","journal-title":"Lwt"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1128\/AEM.06231-11","article-title":"Characterization and comparative genomic analysis of a novel bacteriophage, sfp10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157:H7","volume":"78","author":"Park","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"106","DOI":"10.3390\/foods6120106","article-title":"Effect of food residues in biofilm formation on stainless steel and polystyrene surfaces by Salmonella enterica strains isolated from poultry houses","volume":"6","author":"Paz-M\u00e9ndez","year":"2017","journal-title":"Foods"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"e102","DOI":"10.1002\/cpbi.102","article-title":"Using spades de novo assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"107","DOI":"10.3329\/bjvm.v12i2.21264","article-title":"Poultry Salmonella specific bacteriophage isolation and characterization","volume":"12","author":"Rahaman","year":"2014","journal-title":"Bangl. J. Vet. Med."},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"427","DOI":"10.3109\/1040841X.2012.723675","article-title":"Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics","volume":"39","author":"Rodr\u00edguez-Rubio","year":"2013","journal-title":"Critical Reviews in Microbiology."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"W686","DOI":"10.1093\/nar\/gki366","article-title":"The trnascan-se, snoscan and snogps web servers for the detection of tRNAs and snoRNAs","volume":"33","author":"Schattner","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1111\/mmi.13121","article-title":"The Clostridium difficile cell wall protein CWPV confers phase-variable phage resistance","volume":"98","author":"Sekulovic","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.jgar.2022.02.013","article-title":"Draft genome sequences of 25 Salmonella enterica serovar agona strains isolated from poultry and associated food products harbouring multiple antibiotic resistance genes","volume":"29","author":"Siddique","year":"2022","journal-title":"J. Global Antimicrobial Resist."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"3103","DOI":"10.3390\/foods13193103","article-title":"Isolation and characterization of two novel lytic bacteriophages against Salmonella Typhimurium and their biocontrol potential in food products","volume":"13","author":"Song","year":"2024","journal-title":"Foods"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1756","DOI":"10.3390\/foods12091756","article-title":"Human salmonellosis: a continuous global threat in the farm-to-fork food safety continuum","volume":"12","author":"Teklemariam","year":"2023","journal-title":"Foods"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"410","DOI":"10.3389\/fcimb.2017.00410","article-title":"The Espf N-terminal of enterohemorrhagic Escherichia coli O157:H7 EDL933w imparts stronger toxicity effects on HT-29 cells than the C-terminal","volume":"7","author":"Wang","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.virol.2010.03.044","article-title":"Complete genomic sequence analysis of the temperate bacteriophage phiSASD1 of Streptomyces avermitilis","volume":"403","author":"Wang","year":"2010","journal-title":"Virology"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"3349","DOI":"10.1111\/1750-3841.15843","article-title":"Phage and phage lysins: new era of bio-preservatives and food safety agents","volume":"86","author":"Xu","year":"2021","journal-title":"J. Food Sci."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"e217125","DOI":"10.1128\/spectrum.02171-25","article-title":"A novel tail fiber protein triggers phage DNA ejection by recognizing lipopolysaccharides of k54 hypervirulent Klebsiella pneumoniae","volume":"13","author":"Yin","year":"2025","journal-title":"Microbiol. Spectrum"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"114251","DOI":"10.1016\/j.lwt.2022.114251","article-title":"Isolation and characterization of a novel Salmonella bacteriophage JNWZ02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods","volume":"173","author":"Zhang","year":"2023","journal-title":"LWT"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"127699","DOI":"10.1016\/j.micres.2024.127699","article-title":"Isolation, characterization and application of noble bacteriophages targeting potato common scab pathogen Streptomyces stelliscabiei","volume":"283","author":"Zhang","year":"2024","journal-title":"Microbiol. Res."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"111512","DOI":"10.1016\/j.ijfoodmicro.2025.111512","article-title":"Characterization of a novel Klebsiella pneumoniae bacteriophage vb_kpp_hs37 and its endolysin applied in food","volume":"445","author":"Zhu","year":"2026","journal-title":"Int. J. 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