{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:49:17Z","timestamp":1782107357415,"version":"3.54.5"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Wastewater treatment plants (WWTPs) are widely recognized as hotspots for the accumulation of antimicrobial resistance determinants (AMR). This study analyses the presence of antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and antibiotic residues in influent and effluent wastewater, as well as in dehydrated and composted sludge from a municipal WWTP including a composting facility in southern Spain. Influent wastewater exhibited different ARG profiles, including macrolide\u2013lincosamide\u2013streptogramin (MLSB), aminoglycoside, tetracycline, quinolone, and multidrug resistance related genes, alongside MGEs such as\n                    <jats:italic>intI1<\/jats:italic>\n                    ,\n                    <jats:italic>IS26<\/jats:italic>\n                    , and\n                    <jats:italic>qepA<\/jats:italic>\n                    . Although treatment processes reduced microbial loads and the relative abundance of ARGs, several ARGs and MGEs persisted in effluent water. Dehydrated Sludge matrices showed the presence of tetracycline and macrolide antibiotics (e.g., doxycycline and azithromycin), consistent with their physicochemical properties related with their environmental persistence. Composting treatment led to an effective decreasing in the global ARG load, however, key MGEs, including integrons, insertion sequences, and transposons, remained detectable, indicating the persistence of residual genetic reservoirs, which can be justified by the presence of antibiotics at low concentration and\/or co-occurring stressors. The application of resistance metrics, such as the Antibiotic Resistance Gene Index (ARGI) and the Standard Resistance Gene Index (SRGI), enabled comparison with European benchmark data, revealing that ARG loads detected in wastewater and sludge matrices were consistent with previously reported regional data. The detection of clinically relevant genes (e.g.,\n                    <jats:italic>mcr-1<\/jats:italic>\n                    ,\n                    <jats:italic>qacE\u03941<\/jats:italic>\n                    , and\n                    <jats:italic>merA<\/jats:italic>\n                    ) indicates that these matrices may act as reservoirs of resistance determinants of public health concern. Overall, these findings highlight the value of molecular surveillance and resistance indices and the need for longer-term studies of AMR in wastewater and sludge systems.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1830828","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:38:29Z","timestamp":1782106709000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive resistome and antibiotic profiling in wastewater and biosolids: a case study"],"prefix":"10.3389","volume":"17","author":[{"given":"Alba","family":"Lara-Moreno","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Madrid","sequence":"additional","affiliation":[{"name":"Department of Soils and Water, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC)","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Rubio-Bellido","sequence":"additional","affiliation":[{"name":"Department of Soils and Water, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC)","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Borsetto","sequence":"additional","affiliation":[{"name":"Resistomap Oy","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Nurmi","sequence":"additional","affiliation":[{"name":"Resistomap Oy","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Alonso","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Seville","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan L.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Seville","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Villaverde","sequence":"additional","affiliation":[{"name":"Department of Soils and Water, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC)","place":["Seville, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"ref3","doi-asserted-by":"publisher","first-page":"1231938","DOI":"10.3389\/fmicb.2023.1231938","article-title":"Integrons in the development of antimicrobial resistance: critical review and perspectives","volume":"14","author":"Ahmad","year":"2023","journal-title":"Front. 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Methods"},{"key":"ref39","year":"2018"},{"key":"ref40","first-page":"261","volume-title":"Advances in Microbial Physiology","author":"Pal","year":"2017"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"135525","DOI":"10.1016\/j.jhazmat.2024.135525","article-title":"Efficient elimination of antibiotics and antibiotic resistance genes in hyperthermophilic sludge composting","volume":"479","author":"Pan","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"119029","DOI":"10.1016\/j.envpol.2022.119029","article-title":"Restriction of biosolids returning to land: fate of antibiotic resistance genes in soils after long-term biosolids application","volume":"301","author":"Qin","year":"2022","journal-title":"Environ. Pollut."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/s40793-022-00437-x","article-title":"Metagenomic assembly reveals hosts and mobility of common antibiotic resistome in animal manure and commercial compost","volume":"17","author":"Qiu","year":"2022","journal-title":"Environ. Microbiome"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.scitotenv.2013.01.032","article-title":"Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review","volume":"447","author":"Rizzo","year":"2013","journal-title":"Sci. Total Environ."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"40","DOI":"10.3389\/fmicb.2011.00040","article-title":"Environmental macrolide\u2013Lincosamide\u2013streptogramin and tetracycline resistant Bacteria","volume":"2","author":"Roberts","year":"2011","journal-title":"Front. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"172348","DOI":"10.1016\/j.scitotenv.2024.172348","article-title":"Antimicrobial resistance in rural rivers: comparative study of the coquet (Northumberland) and Eden (Cumbria) river catchments","volume":"928","author":"Robins","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref47","volume-title":"Bolet\u00edn Oficial del Estado (BOE), No. 164, 10 July 2013","year":"2013"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"399","DOI":"10.3389\/fmicb.2012.00399","article-title":"Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture","volume":"3","author":"Seiler","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"120814","DOI":"10.1016\/j.envres.2025.120814","article-title":"Investigating the antibiotic resistance genes and mobile genetic elements in water systems impacted with anthropogenic pollutants","volume":"269","author":"Sharma","year":"2025","journal-title":"Environ. Res."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"111259","DOI":"10.1016\/j.ecolind.2023.111259","article-title":"Differentiation of environment and wastewater treatment plants by core antibiotic resistance genes and aadA2 as indicators in South Korea","volume":"157","author":"Shin","year":"2023","journal-title":"Ecol. Indic."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"126096","DOI":"10.1016\/j.envpol.2025.126096","article-title":"High-throughput qPCR profiling of antimicrobial resistance genes and bacterial loads in wastewater and receiving environments","volume":"373","author":"Silvester","year":"2025","journal-title":"Environ. Pollut."},{"key":"ref52","volume-title":"National Plan against Antibiotic Resistance (PRAN) 2022\u20132024","year":"2022"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"fiy130","DOI":"10.1093\/femsec\/fiy130","article-title":"Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements","volume":"94","author":"Stedtfeld","year":"2018","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"161386","DOI":"10.1016\/j.scitotenv.2023.161386","article-title":"Antibiotic and heavy metal resistance genes in sewage sludge survive during aerobic composting","volume":"866","author":"Tan","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"388","DOI":"10.3390\/biology10050388","article-title":"The role of Nitroreductases in resistance to nitroimidazoles","volume":"10","author":"Thomas","year":"2021","journal-title":"Biology (Basel)"},{"key":"ref9003","year":"2023"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"567","DOI":"10.3390\/antibiotics4040567","article-title":"Co-selection of resistance to antibiotics, biocides and heavy metals, and its relevance to foodborne pathogens","volume":"4","author":"Wales","year":"2015","journal-title":"Antibiotics"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"177017","DOI":"10.1016\/j.scitotenv.2024.177017","article-title":"Mechanisms and influencing factors of horizontal gene transfer in composting system: a review","volume":"955","author":"Wang","year":"2024","journal-title":"Sci. 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