{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T07:02:51Z","timestamp":1779692571837,"version":"3.53.1"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Polycyclic aromatic hydrocarbons (PAHs) are environmental contaminants that cause adverse health effects. This study investigated the toxic effect of different molecular weight PAHs on eukaryote model organism (\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    ) exploiting oxidative stress biomarkers as endpoint indicators. Yeast cells were treated with environmentally relevant concentrations of phenanthrene (PHE), fluoranthene (FLA), and benzo[\n                    <jats:italic>ghi<\/jats:italic>\n                    ]perylene (BghiP) for 24\u202fh. Benzo[\n                    <jats:italic>a<\/jats:italic>\n                    ]pyrene (BaP) was used as a positive control. Subsequently, expression of genes related to oxidative stress responses, cytotoxicity via targeted analysis of antioxidant mutants, generation of reactive oxygen species (ROS), superoxide dismutase (SOD) activity, and glutathione (GSH) content were assessed. The results demonstrated that viability of\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    was related to the molecular weight of PAHs. Oxidative stress related genes\n                    <jats:italic>SOD1<\/jats:italic>\n                    and\n                    <jats:italic>SOD2<\/jats:italic>\n                    were found to be prominently expressed. According to the redox mechanism of action, BghiP and BaP triggered ROS formation and induced an increasing activity of SOD. Furthermore, PAH exposure significantly affected the glutathione levels. In summary, PAHs clearly caused oxidative stress. Among PAH samples, the more benzene ring contained more toxicity level exerted in yeast cells.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1832345","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:05:20Z","timestamp":1779689120000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Oxidative stress response of Saccharomyces cerevisiae exposed to different molecular weight of polycyclic aromatic hydrocarbons"],"prefix":"10.3389","volume":"17","author":[{"given":"Pennapa","family":"Takam","sequence":"first","affiliation":[{"name":"Program in Biotechnology, Faculty of Science, Maejo University","place":["Chiang Mai, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasin","family":"Charerntantanakul","sequence":"additional","affiliation":[{"name":"Program in Biotechnology, Faculty of Science, Maejo University","place":["Chiang Mai, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panwad","family":"Sillapawattana","sequence":"additional","affiliation":[{"name":"Program in Environmental Technology, Faculty of Science, Maejo University","place":["Chiang Mai, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1016\/j.toxrep.2024.04.010","article-title":"Genetic and epigenetic modulations in toxicity: the two-sided roles of heavy metals and polycyclic aromatic hydrocarbons from the environment","volume":"12","author":"Adegbola","year":"2024","journal-title":"Toxicol. Rep."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s13369-021-06236-9","article-title":"Microalgae\u2013Bacteria consortia: a review on the degradation of polycyclic aromatic hydrocarbons (PAHs)","volume":"47","author":"Ahmad","year":"2022","journal-title":"Arab. J. Sci. Eng."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.copbio.2015.12.002","article-title":"Promising approaches towards biotransformation of polycyclic aromatic hydrocarbons with Ascomycota fungi","volume":"38","author":"Aranda","year":"2016","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref4","volume-title":"Air pollution","author":"Byeong Kyu","year":"2010"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0065-2164(08)70052-2","article-title":"Microbial metabolism of polycyclic aromatic hydrocarbons","volume":"30","author":"Cerniglia","year":"1984","journal-title":"Adv. Appl. Microbiol."},{"key":"ref9001","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1006\/taap.2000.9082","article-title":"Benzo [g, h, i] perylene synergistically transactivates benzo [a] pyrene-induced CYP1A1 gene expression by aryl hydrocarbon receptor pathway","volume":"170","author":"Cherng","year":"2001","journal-title":"Toxicology and applied pharmacology"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/s0098-2997(01)00012-7","article-title":"Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases","volume":"22","author":"Costa","year":"2001","journal-title":"Mol. Asp. Med."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"2776","DOI":"10.3390\/app12062776","article-title":"Meta-analysis of a new georeferenced database on polycyclic aromatic hydrocarbons in Western and Central Mediterranean seafood","volume":"12","author":"De Giovanni","year":"2022","journal-title":"Appl. Sci."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"64","DOI":"10.3389\/fonc.2012.00064","article-title":"Oxidative stress and programmed cell death in yeast","volume":"2","author":"Farrugia","year":"2012","journal-title":"Front. Oncol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1007\/s00244-018-0538-6","article-title":"PAHs in water, sediment and biota in an area with port activities","volume":"75","author":"Froehner","year":"2018","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1016\/B978-0-12-386454-3.00911-8","article-title":"Polycyclic aromatic hydrocarbons (PAHs)","volume":"3","author":"Gad","year":"2014","journal-title":"Encycl. Toxicol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.3389\/fmicb.2016.01369","article-title":"Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): a review","volume":"7","author":"Ghosal","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1016\/S0891-5849(99)00176-8","article-title":"Biologic and pharmacologic regulation of mammalian glutathione synthesis","volume":"27","author":"Griffith","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.tiv.2009.09.013","article-title":"Differences in cytotoxicity versus pro-inflammatory potency of different PM fractions in human epithelial lung cells","volume":"24","author":"Gualtieri","year":"2010","journal-title":"Toxicol. Vitro."},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.1093\/acprof:oso\/9780198717478.001.0001","volume-title":"Free Radicals in Biology and medicine","author":"Halliwell","year":"2015"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"105296","DOI":"10.1016\/j.tiv.2021.105296","article-title":"Environmental dose of 16 priority-controlled PAHs mixture induce damages of vascular endothelial cells involved in oxidative stress and inflammation","volume":"79","author":"He","year":"2022","journal-title":"Toxicol. In Vitro"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.redox.2013.07.006","article-title":"Biological markers of oxidative stress: applications to cardiovascular research and practice","volume":"1","author":"Ho","year":"2013","journal-title":"Redox Biol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1007\/s11270-022-05598-6","article-title":"Microbial bioremediation techniques for polycyclic aromatic hydrocarbon (PAHs)\u2014a review","volume":"233","author":"Ismail","year":"2022","journal-title":"Water Air Soil Pollut."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.scitotenv.2004.08.009","article-title":"Atmospheric polycyclic aromatic hydrocarbons: size distribution, estimation of their risk and their depositions to the human respiratory tract","volume":"340","author":"Kameda","year":"2005","journal-title":"Sci Total Env."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1016\/j.envint.2010.04.006","article-title":"Polycyclic aromatic hydrocarbons (PAHs) in different indoor dusts and their potential cytotoxicity based on two human cell lines","volume":"36","author":"Kang","year":"2010","journal-title":"Environ. Int."},{"key":"ref9002","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.toxlet.2018.03.005","article-title":"An in vitro cytotoxicities comparison of 16 priority polycyclic aromatic hydrocarbons in human pulmonary alveolar epithelial cells HPAEpiC","volume":"290","author":"Ke","year":"2018","journal-title":"Toxicol Lett"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.envint.2013.07.019","article-title":"A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects","volume":"60","author":"Kim","year":"2013","journal-title":"Environ. Int."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1002\/j.1460-2075.1994.tb06304.x","article-title":"YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides","volume":"13","author":"Kuge","year":"1994","journal-title":"EMBO J."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1289\/ehp.8584","article-title":"Comparison of oxidative properties, light absorbance, and total and elemental mass concentration of ambient PM2.5 collected at 20 European sites","volume":"114","author":"K\u00fcnzli","year":"2006","journal-title":"Environ. Health Perspect."},{"key":"ref23","volume-title":"Hazardous waste management","author":"LaGrega","year":"2010"},{"key":"ref24","first-page":"624","article-title":"Effect of benzo [a] pyrene on genes related to the cell cycle and cytochrome P450 of Saccharomyces cerevisiae","volume":"13","author":"Lee","year":"2003","journal-title":"Microbiol. Biotechnol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/S13068-022-02127-9","article-title":"Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains","volume":"15","author":"Li","year":"2022","journal-title":"Biotechnol. Biofuels Bioprod."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"12854","DOI":"10.1021\/ja027251n","article-title":"Formation of adenine\u2212 n3\/guanine\u2212 n7 cross-link in the reaction of trans-oriented platinum substrates with dinucleotides","volume":"124","author":"Liu","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"736837","DOI":"10.1155\/2012\/736837","article-title":"Glutathione homeostasis and functions: potential targets for medical interventions","volume":"2012","author":"Lushchak","year":"2012","journal-title":"Amino Acids"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"64","DOI":"10.3109\/1040841X.2010.512268","article-title":"Role of oxygenases in guiding diverse metabolic pathways in the bacterial degradation of low-molecular-weight polycyclic aromatic hydrocarbons: a review","volume":"37","author":"Mallick","year":"2011","journal-title":"Crit. Rev. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1111\/mmi.14062","article-title":"Bioremediation and microbial metabolism of benzo (a) pyrene","volume":"109","author":"Ostrem Loss","year":"2018","journal-title":"Mol. Microbiol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2484","DOI":"10.3390\/microorganisms12122484","article-title":"Advances in the degradation of polycyclic aromatic hydrocarbons by yeasts: a review","volume":"12","author":"Padilla-Garfias","year":"2024","journal-title":"Microorganisms"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.3390\/jof8111150","article-title":"DhDIT2 encodes a Debaryomyces hansenii cytochrome P450 involved in benzo (a) pyrene degradation\u2014a proposal for mycoremediation","volume":"8","author":"Padilla-Garfias","year":"2022","journal-title":"J. Fungi"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"3241","DOI":"10.1016\/S1352-2310(01)00080-2","article-title":"Atmospheric distribution of polycyclic aromatic hydrocarbons and deposition to Galveston Bay, Texas, USA","volume":"35","author":"Park","year":"2001","journal-title":"Atmos. Environ."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"562813","DOI":"10.3389\/fmicb.2020.562813","article-title":"Polycyclic aromatic hydrocarbons: sources, toxicity, and remediation approaches","volume":"11","author":"Patel","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1016\/j.cell.2021.01.043","article-title":"Hallmarks of environmental insults","volume":"184","author":"Peters","year":"2021","journal-title":"Cell"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.orggeochem.2012.08.007","article-title":"Polycyclic aromatic hydrocarbon fingerprints in the Pichavaram mangrove\u2013estuarine sediments, southeastern India","volume":"53","author":"Ranjan","year":"2012","journal-title":"Org. Geochem."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"9358","DOI":"10.1038\/s41598-023-35586-7","article-title":"Assessing the cytotoxicity of aerosolized carbon black and benzo [a] pyrene with controlled physical and chemical properties on human lung epithelial cells","volume":"13","author":"Ryu","year":"2023","journal-title":"Sci. Rep."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"2199511","DOI":"10.1080\/23311916.2023.2199511","article-title":"A review of the sources, distribution sequences, and health risks associated with exposure to atmospheric polycyclic aromatic hydrocarbons","volume":"10","author":"Safo-Adu","year":"2023","journal-title":"Cogent Eng."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"2033","DOI":"10.1007\/s00203-020-01929-5","article-title":"A comprehensive review of metabolic and genomic aspects of PAH-degradation","volume":"202","author":"Sakshi","year":"2020","journal-title":"Arch. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"17","DOI":"10.3390\/toxics5030017","article-title":"Do 16 polycyclic aromatic hydrocarbons represent PAH air toxicity?","volume":"5","author":"Samburova","year":"2017","journal-title":"Toxics"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.freeradbiomed.2023.12.033","article-title":"Oxidative stress related effect of xenobiotics on eukaryotic model organism, Saccharomyces cerevisiae","volume":"212","author":"Sillapawattana","year":"2024","journal-title":"FRBM"},{"key":"ref42","doi-asserted-by":"crossref","DOI":"10.1201\/9780203646830","volume-title":"Chemical Pesticides mode of action and Toxicology","author":"Stenersen","year":"2004"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/BF01576368","article-title":"Detoxification of polycyclic aromatic hydrocarbons by fungi","volume":"9","author":"Sutherland","year":"1992","journal-title":"Ind. Microbiol. Biotechnol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1186\/s12302-024-01003-7","article-title":"Toxic effect of polycyclic aromatic hydrocarbons (PAHs) on co-culture model of human alveolar epithelial cells (A549) and macrophages (THP-1)","volume":"36","author":"Takam","year":"2024","journal-title":"Environ. Sci. Eur."},{"key":"ref45","volume-title":"Principles of Ecotoxicology","author":"Walker","year":"2012"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1093\/toxres\/tfaa072","article-title":"Co-culture of human alveolar epithelial (A549) and macrophage (THP-1) cells to study the potential toxicity of ambient PM2. 5: a comparison of growth under ALI and submerged conditions","volume":"9","author":"Wang","year":"2020","journal-title":"Toxicol Res (Camb)"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"113993","DOI":"10.1016\/j.ijheh.2022.113993","article-title":"Assessment of the association of exposure to polycyclic aromatic hydrocarbons, oxidative stress, and inflammation: a cross-sectional study in Augsburg, Germany","volume":"244","author":"Wu","year":"2022","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref9003","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.aquatox.2008.09.004","article-title":"Enhancement of hypoxia-induced gene expression in fish liver by the aryl hydrocarbon receptor (AhR) ligand, benzo [a] pyrene (BaP)","volume":"90","author":"Yu","year":"2008","journal-title":"Aquatic toxicology"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"100177","DOI":"10.1016\/j.jtauto.2022.100177","article-title":"Effect of polycyclic aromatic hydrocarbons on immunity","volume":"5","author":"Yu","year":"2022","journal-title":"J. Transl. Autoimmun."},{"key":"ref9004","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.toxlet.2016.05.023","article-title":"Benzo [ghi] perylene activates the AHR pathway to exert biological effects on the NL-20 human bronchial cell line","volume":"256","author":"Zaragoza-Ojeda","year":"2016","journal-title":"Toxicology letters"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1832345\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:05:25Z","timestamp":1779689125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1832345\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,25]]},"references-count":52,"alternative-id":["10.3389\/fmicb.2026.1832345"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2026.1832345","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,25]]},"article-number":"1832345"}}