{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:10:17Z","timestamp":1790741417133,"version":"4.1.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Multidrug-resistant\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      (MDR-TB) poses a severe global health threat, yet the contribution of cell envelope barriers to intrinsic drug tolerance remains poorly understood. The ABC protein\n                      <jats:italic>Rv0820<\/jats:italic>\n                      is conserved across mycobacteria but functionally uncharacterized, presenting a structural paradox: it lacks canonical transmembrane helices, making conventional efflux pump function mechanistically implausible.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using CRISPR interference (CRISPRi), we silenced\n                      <jats:italic>Ms_5779<\/jats:italic>\n                      \u2014the\n                      <jats:italic>Mycobacterium smegmatis (M. smegmatis)<\/jats:italic>\n                      ortholog of\n                      <jats:italic>Rv0820<\/jats:italic>\n                      \u2014to two graded depths (81% and 69% repression) and subjected the resulting strains to systematic phenotypic interrogation spanning drug susceptibility, cell envelope permeability, biofilm formation, multi-stress tolerance, and macrophage infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Ms_5779<\/jats:italic>\n                      silencing did not affect growth kinetics but markedly disrupted cell envelope integrity, as evidenced by impaired biofilm formation, dissipated membrane potential, and increased ethidium bromide permeability. Silenced strains exhibited broad-spectrum drug hypersusceptibility across six antibiotic classes, with the most striking effect being a 32-fold reduction in norfloxacin MIC, alongside heightened sensitivity to oxidative, acidic, and thermal stress. In a RAW264.7 macrophage infection model,\n                      <jats:italic>Ms_5779<\/jats:italic>\n                      silencing attenuated intracellular survival, impaired host cholesterol exploitation and foamy macrophage induction, and reduced apoptosis induction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Collectively, these findings strongly suggest that\n                      <jats:italic>Ms_5779<\/jats:italic>\n                      \/Rv0820 is a critical functional contributor indispensable for the maintenance of cell envelope integrity, presenting a distinct mechanism from a conventional efflux pump. Targeting this essential uncharacterized ABC protein would simultaneously compromise the drug permeability barrier and the intracellular niche, nominating\n                      <jats:italic>Rv0820<\/jats:italic>\n                      as a mechanistically distinct therapeutic target for anti-tuberculosis drug discovery.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1835283","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T05:41:55Z","timestamp":1780983715000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["CRISPRi-mediated silencing of the ABC transporter Ms_5779 compromises cell envelope integrity and impairs intracellular host lipid exploitation"],"prefix":"10.3389","volume":"17","author":[{"given":"Yuhao","family":"Song","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didi","family":"Ban","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naichao","family":"Diao","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanli","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]},{"name":"Jilin Province Sika Deer Efficient Breeding and Product Development Technology Engineering Research Center, Jilin Agricultural University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Du","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Materials, Jilin Agricultural University","place":["Changchun, China"]},{"name":"Jilin Province Sika Deer Efficient Breeding and Product Development Technology Engineering Research Center, Jilin Agricultural University","place":["Changchun, China"]},{"name":"College of Agriculture, Yanbian University","place":["Yanji, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"001260","DOI":"10.1099\/mic.0.001260","article-title":"A NiCoT family metal transporter of Mycobacterium tuberculosis (Rv2856\/NicT) behaves as a drug efflux pump that facilitates cross-resistance to antibiotics","volume":"168","author":"Adhikary","year":"2022","journal-title":"Microbiology (Reading)"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"fnad114","DOI":"10.1093\/femsle\/fnad114","article-title":"ABC superfamily transporter Rv1273c of Mycobacterium tuberculosis acts as a multidrug efflux pump","volume":"370","author":"Adhikary","year":"2023","journal-title":"FEMS Microbiol. 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