{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:11:26Z","timestamp":1784099486433,"version":"3.55.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      A LexA-mediated biosensor tailored for space applications was developed leveraging the desert cyanobacterium\n                      <jats:italic>Chroococcidiopsis<\/jats:italic>\n                      sp. CCMEE 029 engineered with a transcriptional fusion between an SOS-responsive promoter and a luciferase gene reporter.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The SOS DNA repair system was investigated by bioinformatics analysis that identified a\n                      <jats:italic>lexA<\/jats:italic>\n                      gene in the genome CCMEE 029 encoding a protein sharing key structural features with other cyanobacterial homologs. Consensus LexA-binding motifs upstream genes involved in DNA-damage repair, photosynthesis, and oxidative-stress defense were computationally identified and the DNA binding of CCMEE 029\u2019 LexA was confirmed by molecular modeling and molecular dynamics simulations. The expression of\n                      <jats:italic>lexA<\/jats:italic>\n                      and\n                      <jats:italic>recA<\/jats:italic>\n                      after treatment for 30 min with 10 mM H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      was evaluated by RT-qPCR. Then Chroococcidiopsis sp. CCMEE 029 was transformed with a plasmid carrying a transcriptional fusion between the upstream region of the\n                      <jats:italic>recA<\/jats:italic>\n                      gene containing the LexA-binding motif and a firefly luciferase gene. The biosensor responsiveness was tested in response to DNA-damaging agents, after desiccation as well as under simulated microgravity conditions combined with \u03b3-rays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Upon the addition of the luciferin substrate, Chroococcidiopsis transformants exposed to DNA-damaging agents (H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      , ultra-violet C (UVC), and \u03b3-ray irradiation) emitted a bioluminescent signal. A correlation was detected between increased UVC doses and the onset of detectable DNA damage. The biosensor responsiveness was confirmed under simulated microgravity conditions combined with \u03b3-rays used as a proxy of the space environment. Notably, the biosensor responsiveness was retained after 4.5 months of air-dried storage as demonstrated by signal emission after rehydration and UVC and \u03b3-ray irradiation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Although a more global stress management role for LexA remains to be investigated in Chroococcidiopsis sp. CCMEE 029. The tested biosensor\u2019s feature supports a future integration of the air-dried biosensor into satellites and its in-orbit reactivation for real-time monitoring of the effects of space conditions, thus advancing future space exploration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1842062","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:43:52Z","timestamp":1784097832000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Harnessing desiccation-tolerant cyanobacteria as biosensors for LexA-mediated responses in deep space"],"prefix":"10.3389","volume":"17","author":[{"given":"Costanza Maria","family":"Martella","sequence":"first","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]},{"name":"PhD Program in Cellular and Molecular Biology, University of Rome \u201cTor Vergata\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Di Stefano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Chirico","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]},{"name":"PhD Program in Cellular and Molecular Biology, University of Rome \u201cTor Vergata\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"D\u2019Agostino","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]},{"name":"PhD Program in Cellular and Molecular Biology, University of Rome \u201cTor Vergata\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Maria","family":"D\u2019Andrea","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio","family":"Colini","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]},{"name":"PhD Program in Cellular and Molecular Biology, University of Rome \u201cTor Vergata\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Iacovelli","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Falconi","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]},{"name":"Bioinformatics Research Unit in Infectious Diseases, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Verna","sequence":"additional","affiliation":[{"name":"ENEA-Nuclear Department, Casaccia Research Center","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Scifo","sequence":"additional","affiliation":[{"name":"ENEA-Nuclear Department, Casaccia Research Center","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Cemmi","sequence":"additional","affiliation":[{"name":"ENEA-Nuclear Department, Casaccia Research Center","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Billi","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata,\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1134\/S1022795420060022","article-title":"Specific lux biosensors of Escherichia coli containing pRecA::lux, pColD::lux, and pDinI::lux plasmids for detection of genotoxic agents","volume":"56","author":"Abilev","year":"2020","journal-title":"Russ. 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