{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:47:00Z","timestamp":1782460020759,"version":"3.54.5"},"reference-count":74,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Bacteriophages are ubiquitous in nature, but relatively few have been isolated and characterized compared to the number of bacterial strains. Phage biotechnology applications benefit from a diverse library of isolated phages to kill or transfer genetic material to a bacterium of interest. However, scaling up phage discovery for diverse bacterial hosts can be time-consuming and costly. We developed an approach to capture novel phages for multiple bacterial strains in parallel from an environmental sample using commercially available 0.2-\u03bcM filter plates. Using this High-throughput Phage Isolation Platform (HtPIP), 12 novel phages were isolated spanning 9 diverse bacterial host genera. Eleven of the isolated phages define new phage species, with nine also defining new genera. The HtPIP was used to discover both DNA and RNA phages, including a\n                    <jats:italic>Tectiviridae<\/jats:italic>\n                    infecting\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    mt-2 and a\n                    <jats:italic>Leviviricetes<\/jats:italic>\n                    infecting a\n                    <jats:italic>Microbacterium<\/jats:italic>\n                    isolate, which represents the first cultured RNA phage infecting a host outside of Proteobacteria. Using a metagenomic approach, we demonstrate that the HtPIP captures a higher proportion of novel phages compared to traditional low-throughput methods.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1845440","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T14:59:09Z","timestamp":1781794749000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["HtPIP: High-throughput phage isolation platform increases diversity and reduces isolation time using multiple bacteria"],"prefix":"10.3389","volume":"17","author":[{"given":"Ben","family":"Diaz","sequence":"first","affiliation":[{"name":"Department of Bioengineering and Biotechnology, Sandia National Laboratories","place":["Livermore, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa","family":"House","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biotechnology, Sandia National Laboratories","place":["Livermore, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghana","family":"Padala","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biotechnology, Sandia National Laboratories","place":["Livermore, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph S.","family":"Schoeniger","sequence":"additional","affiliation":[{"name":"Biological, Radiation, and Signature Science, Technology, and Engineering Center, Sandia National Laboratories","place":["Livermore, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Mageeney","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biotechnology, Sandia National Laboratories","place":["Livermore, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e1004636","DOI":"10.1371\/journal.pgen.1004636","article-title":"Developmentally-regulated excision of the SP\u03b2 prophage reconstitutes a gene required for spore envelope maturation in Bacillus subtilis","volume":"10","author":"Abe","year":"2014","journal-title":"PLoS Genet."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1038\/nbt.4163","article-title":"KBase: the United States department of energy systems biology knowledgebase","volume":"36","author":"Arkin","year":"2018","journal-title":"Nat. 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