{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T07:05:37Z","timestamp":1779433537832,"version":"3.53.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    This study evaluates the biotechnological potential of\n                    <jats:italic>Lachancea thermotolerans<\/jats:italic>\n                    , a non-\n                    <jats:italic>Saccharomyces<\/jats:italic>\n                    yeast species commonly characterized by its ability to produce L-lactic acid, for its application in Spanish-style table olive fermentation. A total of sixty-six\n                    <jats:italic>L. thermotolerans<\/jats:italic>\n                    strains were assessed under varying pH and NaCl conditions, two key stress factors in table olive processing. Growth performance was determined through the analysis of growth curves and compared with those of\n                    <jats:italic>Wickerhamomyces anomalus<\/jats:italic>\n                    and\n                    <jats:italic>Candida boidinii<\/jats:italic>\n                    , two yeast species commonly isolated from table olive fermentations. To characterize strain behavior under stress conditions, growth parameters derived from the models were further analyzed using Principal Component Analysis and hierarchical clustering. These approaches enabled the classification of strains according to their tolerance to pH and salt stress, as well as the identification of groups with distinct growth kinetics. The results revealed significant variability among\n                    <jats:italic>L. thermotolerans<\/jats:italic>\n                    strains, highlighting a strain-dependent response to environmental stressors. A subset of strains exhibited high tolerance to both pH and NaCl conditions, demonstrating stable growth performance and suggesting a strong adaptive capacity. These findings support the potential application of selected\n                    <jats:italic>L. thermotolerans<\/jats:italic>\n                    strains as promising candidates for controlled table olive fermentation processes.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1846416","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T06:12:18Z","timestamp":1779430338000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating Lachancea thermotolerans for table olive fermentation: performance under pH and NaCl stress conditions"],"prefix":"10.3389","volume":"17","author":[{"given":"Patricia","family":"Gil-Flores","sequence":"first","affiliation":[{"name":"Departamento de Ciencias Biom\u00e9dicas (\u00c1rea de Microbiolog\u00eda), Facultad de Ciencias, Universidad de Extremadura","place":["Badajoz, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Penco-Parra","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Biom\u00e9dicas (\u00c1rea de Microbiolog\u00eda), Facultad de Ciencias, Universidad de Extremadura","place":["Badajoz, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Bautista-Gallego","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Biom\u00e9dicas (\u00c1rea de Microbiolog\u00eda), Facultad de Ciencias, Universidad de Extremadura","place":["Badajoz, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref2","doi-asserted-by":"publisher","first-page":"34","DOI":"10.3389\/fmicb.2012.00161","article-title":"Potential benefits of the application of yeast starters in table olive processing","volume":"3","author":"Arroyo-L\u00f3pez","year":"","journal-title":"Front. 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Food Sci."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.foodcont.2011.08.020","article-title":"Modeling the inhibitory effects of zinc chloride on table olive related yeasts","volume":"23","author":"Bautista-Gallego","year":"2012","journal-title":"Food Control"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"135","DOI":"10.17113\/ftb.54.02.16.4220","article-title":"Quality and composition of Airen wines fermented by sequential inoculation of Lachancea thermotolerans and Saccharomyces cerevisiae","volume":"54","author":"Benito","year":"2016","journal-title":"Food Technol. Biotechnol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3390\/microorganisms5020030","article-title":"Table olive fermentation using starter cultures with multifunctional potential","volume":"5","author":"Bonatsou","year":"2017","journal-title":"Microorganisms"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.32614\/CRAN.package.pracma","article-title":"Pracma: practical numerical math functions (R package) (2.4.6)","volume":"395","author":"Borchers","year":"2025","journal-title":"CRAN R Project"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/s13197-018-3468-4","article-title":"Characterization of antimicrobial activities of olive phenolics on yeasts using conventional methods and mid-infrared spectroscopy","volume":"56","author":"Canal","year":"2019","journal-title":"J. Food Sci. 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Pack."},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4899-4683-6","volume-title":"Table Olives","author":"Fernandez","year":"1997"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1111\/j.1567-1364.2008.00427.x","article-title":"Wine yeasts for the future","volume":"8","author":"Fleet","year":"2008","journal-title":"FEMS Yeast Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.ijfoodmicro.2009.08.018","article-title":"Presence of lactic bacteria in the air of a winery during the vinification period","volume":"136","author":"Garijo","year":"2009","journal-title":"Int. J. 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R Package Version 2.0.0","author":"Kassambara","year":"2026"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1046\/j.1365-2672.2000.01017.x","article-title":"Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values","volume":"88","author":"Lambert","year":"2000","journal-title":"J. Appl. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"7463","DOI":"10.1038\/s41598-022-10743-6","article-title":"7000-year-old evidence of fruit tree cultivation in the Jordan Valley, Israel","volume":"12","author":"Langgut","year":"2022","journal-title":"Sci. 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Sci. Food Agric."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1038\/s41538-025-00467-y","article-title":"Fermentative factors shape transcriptional response of Lachancea thermotolerans and wine acidification","volume":"9","author":"Vicente","year":"2025","journal-title":"NPJ Sci. Food"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s10482-010-9491-8","article-title":"Pichia anomala: cell physiology and biotechnology relative to other yeasts","volume":"99","author":"Walker","year":"2011","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.ijfoodmicro.2012.03.003","article-title":"Detection of Escherichia coli O157:H7 and Salmonella in ground beef by a bead-free quantum dot-facilitated isolation method","volume":"156","author":"Wang","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s10295-018-2107-4","article-title":"Improvement of thermotolerance in Lachancea thermotolerans using a bacterial selection pressure","volume":"46","author":"Zhou","year":"2019","journal-title":"J. Ind. Microbiol. 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