{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T05:46:00Z","timestamp":1785735960323,"version":"3.56.0"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Provincial Program on Platform and Talent Development of the Department of Science and Technology of Guizhou, China","award":["[2019] 5617"],"award-info":[{"award-number":["[2019] 5617"]}]},{"DOI":"10.13039\/100016698","name":"Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province","doi-asserted-by":"crossref","award":["[2019] 5655"],"award-info":[{"award-number":["[2019] 5655"]}],"id":[{"id":"10.13039\/100016698","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016698","name":"Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province","doi-asserted-by":"crossref","award":["U1812401"],"award-info":[{"award-number":["U1812401"]}],"id":[{"id":"10.13039\/100016698","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Bacterial resistance to bacteriophages represents a major limitation for the durable use of phage-based biocontrol strategies in agriculture. Here, we used a phage-resistance derivative of\n                    <jats:italic>Xanthomonas campestris<\/jats:italic>\n                    pv.\n                    <jats:italic>campestris<\/jats:italic>\n                    (\n                    <jats:italic>Xcc<\/jats:italic>\n                    ) as a selective host to isolate an additional phage with activity against resistant bacteria. A spontaneous mutant, designated\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004R, was isolated following exposure of\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004 to phage X1.\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004R exhibited a growth profile comparable to that of the wild-type strain, but differed in colony appearance, X1 adsorption efficiency, and motility phenotypes. Whole-genome resequencing identified mutations in genes annotated as encoding a lipopolysaccharide (LPS) core biosynthesis protein, PilY1, N-acetylmuramoyl-L-alanine amidase, and IS1404 transposase, and a conserved hypothetical protein. Using\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004R as the isolation host, we recovered a bacteriophage, vB_Xcc_GYRb1 (GYRb1), from agricultural soil. GYRb1 infected both\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004 and\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004R and exhibited different adsorption kinetics on the two strains. Transmission electron microscopy showed an icosahedral head and a long, non-contractile tail. Genome sequencing revealed a circularly assembled 91,478-bp double-stranded DNA genome encoding 128 predicted open reading frames and two tRNAs. No recognizable antibiotic resistance or bacterial virulence genes were detected. Phylogenomic, ANI, and gene-sharing network analyses suggest that GYRb1 is highly divergent from currently available related phages and may represent a candidate genus-level lineage within the class\n                    <jats:italic>Caudoviricetes<\/jats:italic>\n                    . GYRb1 suppressed the growth of\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004 and\n                    <jats:italic>Xcc<\/jats:italic>\n                    8004R\n                    <jats:italic>in vitro<\/jats:italic>\n                    and reduced disease severity in cabbage leaf. A cocktail containing GYRb1 and X1 reduced OD\n                    <jats:sub>600<\/jats:sub>\n                    -based bacterial regrowth compared with single-phage treatments\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Together, these findings support the feasibility of using phage-resistant bacterial mutants as selective hosts to obtain complementary candidate phages, while highlighting the need for receptor identification, resistance-frequency analysis, lysogeny assessment, and broader host-range testing before practical application.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1853113","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T05:30:30Z","timestamp":1785735030000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation of a complementary bacteriophage from a phage-resistant mutant expands control strategies against Xanthomonas campestris"],"prefix":"10.3389","volume":"17","author":[{"given":"Qing","family":"Yu","sequence":"first","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingshan","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruina","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Agriculture, Qiannan Normal University for Nationalities","place":["Duyun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leitao","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangen","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingbei","family":"Weng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University","place":["Guiyang, China"]},{"name":"School of Biological Sciences and Agriculture, Qiannan Normal University for Nationalities","place":["Duyun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"D690","DOI":"10.1093\/nar\/gkac920","article-title":"CARD 2023: expanded curation, support for machine learning, and resistome prediction at the comprehensive antibiotic resistance database","volume":"51","author":"Alcock","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.femsre.2004.12.008","article-title":"The many faces of the helix-turn-helix domain: transcription regulation and beyond","volume":"29","author":"Aravind","year":"2005","journal-title":"FEMS Microbiol. 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