{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:47:19Z","timestamp":1785822439821,"version":"3.56.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Influenza B virus (IBV) is a major etiological agent of seasonal influenza, imposing a significant global health burden. Rapid and accurate detection is crucial for epidemic control, but conventional methods are limited by complex equipment or long turnaround times.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Herein, we developed a reverse transcription recombinase-aided amplification assay combined with lateral flow dipstick (RT-RAA-LFD) for specific IBV detection, targeting the conserved\n                      <jats:italic>ns<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following primer screening, the optimal primer pair F2\/R4 was identified. The assay was optimized to react at 39\u00b0C for 20 min with a detection volume of 15 \u03bcL. Analytical validation demonstrated high sensitivity (50 copies\/mL) and no nonspecific cross-reactivity with other common respiratory viruses, including influenza A virus, SARS-CoV-2, parainfluenza virus and adenovirus. Clinical performance evaluation revealed 100% concordance with the gold standard PCR assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Collectively, the established RT-RAA-LFD assay is rapid, specific, sensitive and easy to operate without relying on sophisticated instruments, making it a promising tool for point-of-care testing (POCT) and field surveillance of IBV infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1856881","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:36:43Z","timestamp":1785821803000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a rapid, sensitive and visual reverse transcription recombinase-aided amplification coupled with lateral flow dipstick assay for influenza B virus detection"],"prefix":"10.3389","volume":"17","author":[{"given":"Yanhong","family":"Liu","sequence":"first","affiliation":[{"name":"QT Biotech Co., Ltd","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heyuan","family":"Geng","sequence":"additional","affiliation":[{"name":"Jiangsu International Travel Health Care Center (Nanjing Customs Port Outpatient Department)","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shaanxi International Travel Healthcare Center (Xi\u2019an Customs Port Outpatient Department)","place":["Xi\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanchao","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Jiangnan University Medical Center","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Sun","sequence":"additional","affiliation":[{"name":"Wuxi Customs, Wuxi","place":["Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinggui","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu International Travel Health Care Center (Nanjing Customs Port Outpatient Department)","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuxi Customs, Wuxi","place":["Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingdi","family":"Pan","sequence":"additional","affiliation":[{"name":"Wuxi Customs, Wuxi","place":["Jiangsu, China"]},{"name":"School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"199089","DOI":"10.1016\/j.virusres.2023.199089","article-title":"Detection of reassortant influenza B strains from 2004 to 2015 seasons in Barcelona (Catalonia, Spain) by whole genome sequencing.","volume":"330","author":"Andr\u00e9s","year":"2023","journal-title":"Virus Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e0053525","DOI":"10.1128\/jcm.00535-25","article-title":"An extraction-free, lyophilized one-pot RAA-CRISPR assay for point-of-care testing of Haemophilus influenzae.","volume":"63","author":"Cao","year":"2025","journal-title":"J. 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