{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:00:47Z","timestamp":1781244047565,"version":"3.54.1"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Pumpkin production in Guangxi, China, is increasingly affected by complex viral diseases, but the regional viral spectrum and mixed-infection structure in symptomatic pumpkin plants remain insufficiently characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed 289 symptomatic pumpkin samples collected across Guangxi during 2022-2025 using small-RNA sequencing for virus discovery and targeted PCR\/RT-PCR assays for individual-sample confirmation. Virus prevalence, mixed-infection profiles, regional and host-associated differences, and virus-virus and symptom-virus associations were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Small-RNA sequencing identified 15 candidate virus species, and targeted assays confirmed 11 viruses. Among the 289 samples, 284 (98.27%) were positive for at least one confirmed virus and 279 (96.54%) carried two or more viruses. The dataset was dominated by PuYmMV, SLCPHV, TLCCNV, ToLCNDV, and SLCCNV, which were detected in 87.89%, 87.89%, 83.39%, 78.89%, and 68.86% of samples, respectively. The most frequent infection profile was simultaneous detection of these five begomovirus species, occurring in 35.64% of samples. After Benjamini-Hochberg correction, ZTMV and PRSV were enriched in northern Guangxi, whereas TLCCNV and ToLCNDV were enriched in southern Guangxi. Host-species differences were not significant after correction. Pairwise analysis revealed a strong begomovirus-rich co-detection module, whereas symptom-virus associations were comparatively weak.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Symptomatic pumpkin viral disease in Guangxi is characterized by broad virus diversity, pervasive mixed infection, and recurrent begomovirus-rich co-detection. These findings provide a regional baseline for multiplex diagnosis, begomovirus-oriented surveillance, and future plant-vector-reservoir studies in pumpkin production systems.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1857304","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T05:48:48Z","timestamp":1781243328000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Pervasive mixed infections and recurrent begomovirus co-detection in symptomatic pumpkin plants from Guangxi, China"],"prefix":"10.3389","volume":"17","author":[{"given":"Baoling","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenglin","family":"Wan","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Guangxi Academy of Agricultural Sciences, Nanning","place":["Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"27","DOI":"10.6090\/jarq.23J21","article-title":"A multiplex RT-PCR assay for the detection of nine virus species infecting cucumber in Japan","volume":"59","author":"Adachi-Fukunaga","year":"2025","journal-title":"Jpn. 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