{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:02:37Z","timestamp":1790949757126,"version":"4.1.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Avibacterium paragallinarum<\/jats:italic>\n                    (\n                    <jats:italic>A. paragallinarum<\/jats:italic>\n                    ) is one of the primary pathogens causing respiratory diseases in poultry flocks, posing a serious threat to the health and production efficiency of the global poultry industry. With the expansion of modern farming scales and changes in environmental factors, mixed infections involving\n                    <jats:italic>A. paragallinarum<\/jats:italic>\n                    alongside other bacteria and viruses have become increasingly prevalent. This has led to more complex and diverse disease manifestations, significantly increasing the difficulty of prevsention and control. Current research on\n                    <jats:italic>A. paragallinarum<\/jats:italic>\n                    encompasses its antigenic structural characteristics, vaccine development and optimization, and therapeutic strategies. However, gaps remain in the profound understanding of mixed infection mechanisms and synergistic control approaches. This manuscript systematically summarizes recent advances in research concerning\n                    <jats:italic>A. paragallinarum<\/jats:italic>\n                    and its associated bacterial and viral co-infections. It focuses on analyzing the impact of antigenic variation on vaccine efficacy, the status of composite vaccine development, and the pathological mechanisms of multi-pathogen synergistic infection. The aim is to provide a theoretical foundation and practical reference for the comprehensive prevention and control of\n                    <jats:italic>A. paragallinarum<\/jats:italic>\n                    -related diseases, thereby promoting the healthy and sustainable development of the poultry farming industry.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1857529","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:34:51Z","timestamp":1781760891000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Research progress on Avibacterium paragallinarum and related bacterial and viral diseases in poultry and their mixed infections"],"prefix":"10.3389","volume":"17","author":[{"given":"Chen","family":"Mei","sequence":"first","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjiang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University","place":["Yangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingmin","family":"Pan","sequence":"additional","affiliation":[{"name":"Wuhan Institute of Virology, Chinese Academy of Sciences","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baozhu","family":"Xing","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueze","family":"Lyu","sequence":"additional","affiliation":[{"name":"Beijing General Animal Husbandry Station","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1080\/03079450902912143","article-title":"Identification of iron-acquisition proteins of Avibacterium paragallinarum","volume":"38","author":"Abascal","year":"2009","journal-title":"Avian Pathol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1186\/s13567-020-00840-7","article-title":"A transient increase in MHC-IIlow monocytes after experimental infection with Avibacterium paragallinarum (serovar B-1) in SPF chickens","volume":"51","author":"Alvarez","year":"2020","journal-title":"Vet. Res."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1080\/03079457.2023.2239178","article-title":"Molecular characterization of the HMTp210 gene of Avibacterium paragallinarum and the proposition of a new genotyping method as alternative for classical serotyping","volume":"52","author":"Buter","year":"2023","journal-title":"Avian Pathol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.3390\/vaccines10071043","article-title":"Pathogenicity of Avibacterium paragallinarum strains from Peru and the selection of candidate strains for an inactivated vaccine","volume":"10","author":"Caballero-Garcia","year":"2022","journal-title":"Vaccine"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"103751","DOI":"10.1016\/j.psj.2024.103751","article-title":"Prevalence and genomic-based antimicrobial resistance analysis of Avibacterium paragallinarum isolates in Guangdong province, China","volume":"103","author":"Cao","year":"2024","journal-title":"Poult. Sci."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1637\/aviandiseases-D-24-00042","article-title":"Genotypic and biochemical divergence of Avibacterium paragallinarum isolates in China","volume":"69","author":"Chen","year":"2025","journal-title":"Avian Dis."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"110317","DOI":"10.1016\/j.vetmic.2024.110317","article-title":"Identification and characterization of biosynthetic loci of lipooligosaccharide and capsular polysaccharide in Avibacterium paragallinarum","volume":"299","author":"Chen","year":"2024","journal-title":"Vet. Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1080\/03079457.2012.760840","article-title":"Identification of the lic1ABCD operon that controls the phase-variable expression of phosphorylcholine on lipopolysaccharide from Avibacterium paragallinarum","volume":"42","author":"Chiang","year":"2013","journal-title":"Avian Pathol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"105622","DOI":"10.1016\/j.psj.2025.105622","article-title":"Epidemiological investigation of infectious coryza in central China and the effect of Enterococcus faecium on improving vaccine immunity","volume":"104","author":"Cui","year":"2025","journal-title":"Poult. 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Sci."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1637\/11481-081016-Reg","article-title":"Coinfection of Avibacterium paragallinarum and Gallibacterium anatis in specific-pathogen-free chickens complicates clinical signs of infectious coryza, which can be prevented by vaccination","volume":"61","author":"Paudel","year":"2017","journal-title":"Avian Dis."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1111\/zph.12879","article-title":"Surveillance on respiratory diseases reveals enzootic circulation of both H5 and H9 avian influenza viruses in small-scale commercial layer farms of Bangladesh","volume":"68","author":"Rahman","year":"2021","journal-title":"Zoonoses Public Health"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"558349","DOI":"10.3389\/fbioe.2020.558349","article-title":"Chitosan nanoparticle based mucosal vaccines delivered against infectious diseases of poultry and pigs","volume":"8","author":"Renu","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1637\/aviandiseases-D-24-00041","article-title":"Development and validation of PCR assays for improved diagnosis of infectious coryza by differentiating pathogenic and nonpathogenic Avibacterium paragallinarum","volume":"68","author":"Shelkamy","year":"","journal-title":"Avian Dis."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"e0133424","DOI":"10.1128\/mra.01334-24","article-title":"Eight complete and four draft genome sequences of nonpathogenic Avibacterium paragallinarum isolates from naive, healthy layer chickens in the USA","volume":"14","author":"Shelkamy","year":"","journal-title":"Microbiol. Resour. Announc."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1080\/03079457.2024.2323029","article-title":"Case reports involving coinfection with Avibacterium paragallinarum and Ornithobacterium rhinotracheale in broiler chickens and Avibacterium endocarditis in broiler breeding hens in Poland","volume":"53","author":"St\u0119pie\u0144-Py\u015bniak","year":"2024","journal-title":"Avian Pathol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"e0239825","DOI":"10.1128\/aem.02398-25","article-title":"Development of a counterselection system for efficient marker-free genetic manipulation in Avibacterium paragallinarum","volume":"92","author":"Sun","year":"2026","journal-title":"Appl. Environ. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"671","DOI":"10.3390\/vaccines8040671","article-title":"Macrophage activation assays to evaluate the immunostimulatory capacity of Avibacterium paragallinarum in a multivalent poultry vaccine","volume":"8","author":"van den Biggelaar","year":"2020","journal-title":"Vaccine"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"e00392-21","DOI":"10.1128\/AEM.00392-21","article-title":"Stable recombinant-gene expression from a ligilactobacillus live bacterial vector via chromosomal integration","volume":"87","author":"Vezina","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.vetmic.2006.08.034","article-title":"Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library","volume":"119","author":"Wang","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"e1009436","DOI":"10.1371\/journal.ppat.1009436","article-title":"Resident bacteria contribute to opportunistic infections of the respiratory tract","volume":"17","author":"Wu","year":"2021","journal-title":"PLoS Pathog."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"6463","DOI":"10.3382\/ps\/pez531","article-title":"Characterization of emergent Avibacterium paragallinarum strains and the protection conferred by infectious coryza vaccines against them in China","volume":"98","author":"Xu","year":"2019","journal-title":"Poult. Sci."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"e0137922","DOI":"10.1128\/spectrum.01379-22","article-title":"Comparative genomics analysis and outer membrane vesicle-mediated horizontal antibiotic-resistance gene transfer in Avibacterium paragallinarum","volume":"10","author":"Xu","year":"2022","journal-title":"Microbiol. Spectrum"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"2684","DOI":"10.1128\/IAI.69.4.2684-2691.2001","article-title":"Gallinacin-3, an inducible epithelial beta-defensin in the chicken","volume":"69","author":"Zhao","year":"2001","journal-title":"Infect. Immun."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1010584","DOI":"10.3389\/fmicb.2022.1010584","article-title":"Commensal bacteria contribute to the growth of multidrug-resistant Avibacterium paragallinarum in chickens","volume":"13","author":"Zhu","year":"2022","journal-title":"Front. 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