{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:09:50Z","timestamp":1788952190049,"version":"build-2803163510"},"reference-count":75,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Inroduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cobetia marina<\/jats:italic>\n                      is a marine bacterium that sustains growth and biofilm formation across a broad thermal range, serving as an ideal model for exploring adaptability in thermally dynamic and warming oceans. In this study, we investigated how growth temperature reshapes transcriptional regulation and biofilm architecture in\n                      <jats:italic>C. marina<\/jats:italic>\n                      strain MM1IDA2H-1, as well as its trajectories under thermal stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated transcriptomic profiling with confocal and electron microscopy at different growth temperatures. Additionally, we conducted adaptive laboratory evolution (ALE) under progressive heat stress coupled with whole-genome sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Transcriptomes from cultures grown at 16 \u00b0C, 35 \u00b0C, 38 \u00b0C, and 41 \u00b0C were linked to physiology and biofilm structure, whereas ALE revealed strategies under warming stress. Low temperature promoted a biofilm-competent program driving motility and exopolysaccharide production. Conversely, growth at 41 \u00b0C induced a stress-survival state with repression of cooperative traits\u2014quorum sensing\u2014and the activation of DNA repair and oxidative stress responses. Exploratory network analyses predicted NarL, NtrC, CysB, and CsgD as putative components of a temperature-responsive control core, with a reduction in regulatory connectivity at 38 \u00b0C, representing a transitional stop-and-reprogram state. Finally, ALE selected for recurrent clone-specific mutations in\n                      <jats:italic>csgD1<\/jats:italic>\n                      , resolving a phenotypic trade-off by downregulating costly biofilm production to maintain growth capacity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Overall, our findings show how\n                      <jats:italic>C. marina<\/jats:italic>\n                      transitions across a wide temperature range and under thermal stress via state-dependent network rewiring, offering a comprehensive eco-physiological framework that links environmental sensing, multicellular organization, and evolutionary trade-offs in highly dynamic and warming oceans.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1858040","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:14:53Z","timestamp":1788948893000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermal modulation of transcriptional states, biofilm architecture and survival strategies in Cobetia marina"],"prefix":"10.3389","volume":"17","author":[{"given":"Claudia","family":"Ibacache-Quiroga","sequence":"first","affiliation":[{"name":"Escuela de Nutrici\u00f3n y Diet\u00e9tica, Facultad de Farmacia, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]},{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Schmachtenberg","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda, Facultad de Ciencias, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]},{"name":"Centro de Investigaci\u00f3n y Gesti\u00f3n de Recursos Naturales (CIGREN), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karoll","family":"Gonz\u00e1lez-Pizarro","sequence":"additional","affiliation":[{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Herrera","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda, Facultad de Ciencias, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Tapia","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luna","family":"Osses-Lobos","sequence":"additional","affiliation":[{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Serrano-Barba","sequence":"additional","affiliation":[{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]},{"name":"Escuela de Ingenier\u00eda Inform\u00e1tica, Facultad de Ingenier\u00eda, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Stehl\u00edk","sequence":"additional","affiliation":[{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]},{"name":"Instituto de Estad\u00edstica, Facultad de Ciencias, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Alejandro","family":"Dinamarca","sequence":"additional","affiliation":[{"name":"Escuela de Nutrici\u00f3n y Diet\u00e9tica, Facultad de Farmacia, Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]},{"name":"Centro de Micro-Bioinnovaci\u00f3n (CMBi), Universidad de Valpara\u00edso","place":["Valpara\u00edso, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1139\/m92-134","article-title":"Interactive effects of salt concentration and temperature on growth and lipid composition in the moderately halophilic bacterium Vibrio costicola","volume":"38","author":"Adams","year":"1992","journal-title":"Can. J. 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