{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:57:03Z","timestamp":1790575023272,"version":"4.1.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Probiotics have potential benefits in modulating gastrointestinal function. However, their efficacy and application depend largely on specific bacterial strains and environmental contexts. In our previous study, a strain of\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    (\n                    <jats:italic>L. plantarum<\/jats:italic>\n                    ) DC2 exhibited superior functionality in pork fermentation processes and was granted a Chinese patent (ZL202110743486.8). However, its storage and application characteristics remain unclear. To evaluate its direct application potential for gastrointestinal regulation, in this study, the formula and process of lyophilized\n                    <jats:italic>L. plantarum<\/jats:italic>\n                    DC2 powder were optimized via single-factor design (SFD) and response surface methodology (RSM). The optimized protectant consisted of fat-free milk powder, trehalose, glycerine, and L-sodium glutamate. The survival rate and storage stability of lyophilized bacterial powder were maintained at optimal level under optimized conditions. Intragastric administration experiments revealed that the abundance of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    in both the stomach and jejunum significantly increased, accompanied by shifts in intestinal morphological features and modulation of the intestinal microbial composition and abundance. In conclusion, a reliable lyophilization protocol was established for optimal survival rate and storage stability of lyophilized\n                    <jats:italic>L. plantarum<\/jats:italic>\n                    DC2 powder, which was capable of modulating intestinal morphology and gut microbial profiles in mice. This study lays a foundation for its further development as a probiotic candidate.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1858099","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:43:37Z","timestamp":1790574217000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of lyophilized Lactiplantibacillus plantarum DC2 probiotic powder on gastrointestinal morphology and microbiota in mice"],"prefix":"10.3389","volume":"17","author":[{"given":"Juzuo","family":"Zhang","sequence":"first","affiliation":[{"name":"Wuling Mountain Food Engineer Collaborative Innovation Center of Hunan Province\/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province\/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, College of Biological and Food Engineering, Huaihua University","place":["Huaihua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiang","sequence":"additional","affiliation":[{"name":"Joint Surgery Department, Southern Central Hospital of Yunnan Province\/First People's Hospital of Honghe State","place":["Honghe, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuling Mountain Food Engineer Collaborative Innovation Center of Hunan Province\/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province\/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, College of Biological and Food Engineering, Huaihua University","place":["Huaihua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demiao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Joint Surgery Department, Southern Central Hospital of Yunnan Province\/First People's Hospital of Honghe State","place":["Honghe, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfei","family":"Xu","sequence":"additional","affiliation":[{"name":"Wuling Mountain Food Engineer Collaborative Innovation Center of Hunan Province\/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province\/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, College of Biological and Food Engineering, Huaihua University","place":["Huaihua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2192","DOI":"10.1002\/fsn3.3884","article-title":"Clinical trials of probiotics: current outlook","volume":"12","author":"Al-Madhagi","year":"2024","journal-title":"Food Sci. 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