{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:46:54Z","timestamp":1782798414540,"version":"3.54.5"},"reference-count":104,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"\n Pseudomonas aeruginosa<\/jats:italic>\n is a clinically significant bacterial pathogen that poses a serious threat to aquaculture. However, there is limited information on\n Massilia<\/jats:italic>\n isolates against pathogenic\n P. aeruginosa<\/jats:italic>\n in aquaculture. In this study, a potential predator,\n M. varians<\/jats:italic>\n isolate P2-4, was isolated from aquaculture sediment using Chinese mitten crab\n Eriocheir sinensis<\/jats:italic>\n -pathogenic\n P. aeruginosa<\/jats:italic>\n as the prey bacterium, and its genomic features, antibacterial-related genes, virulence genes, toxic metabolites, pathogenicity, antibacterial spectrum, and\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n antibacterial effects against pathogenic\n P. aeruginosa<\/jats:italic>\n in\n E. sinensis<\/jats:italic>\n were further characterized. Isolate P2-4 possessed one chromosome and one plasmid, with a total of 75 tRNAs, 7 5S rRNAs, 7 16S rRNAs, 7 23S rRNAs, 34 sRNAs, 5,238 coding genes, 20 genomic islands, 1 prophage, 23 insertion sequences, and 102 repeat sequences. It harbored 19 antibacterial-related genes (\n pilA<\/jats:italic>\n ,\n pilB<\/jats:italic>\n ,\n pilC<\/jats:italic>\n ,\n pilD<\/jats:italic>\n ,\n pilF<\/jats:italic>\n ,\n pilG<\/jats:italic>\n ,\n pilH<\/jats:italic>\n ,\n pilM<\/jats:italic>\n ,\n pilO<\/jats:italic>\n ,\n pilP<\/jats:italic>\n ,\n pilQ<\/jats:italic>\n ,\n pilS<\/jats:italic>\n ,\n pilR<\/jats:italic>\n ,\n pilT<\/jats:italic>\n ,\n mltA<\/jats:italic>\n ,\n mltB<\/jats:italic>\n ,\n mltC<\/jats:italic>\n ,\n mltD<\/jats:italic>\n , and\n dacB<\/jats:italic>\n ) associated with cellular motility and cell wall lysis. In addition, the isolate carried no virulence genes, was unable to produce hemolysin, hydrogen sulfide, nitrite, and ammonia, and was avirulent in\n E. sinensis<\/jats:italic>\n with a 7-day acute LD\n 50<\/jats:sub>\n value of above 5.0\u202f\u00d7\u202f10\n 8<\/jats:sup>\n \u202fCFU\/mL. Furthermore, the isolate exerted potential antibacterial activity against pathogenic\n Shewanella algae<\/jats:italic>\n ,\n Aeromonas caviae<\/jats:italic>\n ,\n A. hydrophila<\/jats:italic>\n , and\n Photobacterium damselae<\/jats:italic>\n , besides\n P. aeruginosa<\/jats:italic>\n . It exhibited bacteriostatic rates of 99.35 to 99.99% against pathogenic\n P. aeruginosa<\/jats:italic>\n at concentrations of 1.0\u202f\u00d7\u202f10\n 3<\/jats:sup>\n to 1.0\u202f\u00d7\u202f10\n 5<\/jats:sup>\n \u202fCFU\/mL, and displayed relative percentage survivals of 42.31 to 73.08% against\n P. aeruginosa<\/jats:italic>\n infection in\n E. sinensis<\/jats:italic>\n at doses of 6.0\u202f\u00d7\u202f10\n 3<\/jats:sup>\n to 6.0\u202f\u00d7\u202f10\n 5<\/jats:sup>\n \u202fCFU\/g diet. To our knowledge, this study, for the first time, demonstrates a\n M. varians<\/jats:italic>\n strain as a potential biocontrol agent against pathogenic\n P. aeruginosa<\/jats:italic>\n in aquaculture.\n <\/jats:p>","DOI":"10.3389\/fmicb.2026.1863139","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:39:50Z","timestamp":1782797990000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Massilia varians P2-4: a potential biocontrol agent against pathogenic Pseudomonas aeruginosa in Eriocheir sinensis"],"prefix":"10.3389","volume":"17","author":[{"given":"Yiyao","family":"Liu","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueqi","family":"Yang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University","place":["Shanghai, China"]},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Liu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University","place":["Shanghai, China"]},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Quzhou Affiliated Hospital of Wenzhou Medical University","place":["Quzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Cao","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University","place":["Shanghai, China"]},{"name":"Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Gai","sequence":"additional","affiliation":[{"name":"Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Quzhou Affiliated Hospital of Wenzhou Medical University","place":["Quzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.14202\/vetworld.2017.34-37","article-title":"Incidence and virulence characteristics of Aeromonas spp. in fish","volume":"10","author":"Abd-El-Malek","year":"2017","journal-title":"Vet. 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