{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:54:40Z","timestamp":1781848480853,"version":"3.54.5"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Methanol is an ideal feedstock for biomanufacturing and can be produced in large quantities from carbon dioxide via photocatalysis or electrolytic reduction, contributing to global carbon neutrality. In this study, we described an engineered\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    strain capable of efficiently producing the monoterpene 1,8-cineole from methanol. First, a remarkable high-level synthesis of 1,8-cineole was achieved in\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    by introducing an exogenous 1,8-cineole synthase, strengthening the MVA pathway, and suppressing the competing pathway. Subsequently, we significantly improved the yield of 1,8-cineole by reconstructing the methanol assimilation pathway and regulating the non-oxidative branch of the pentose phosphate pathway. To mitigate formaldehyde toxicity and redirect carbon flux toward central carbon metabolism for enhanced cellular vitality, the conversion of formic acid to serine was strengthened by overexpressing the\n                    <jats:italic>ADE3<\/jats:italic>\n                    and\n                    <jats:italic>SHM2<\/jats:italic>\n                    genes, resulting in an 82.2% increase in 1,8-cineole yield. Finally, we obtained 386.3 mg\/L of 1,8-cineole from methanol in a 5 L bioreactor. This study established a metabolic engineering platform for the efficient synthesis of 1,8-cineole in\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    based on methanol assimilation, providing a new strategy for the green biomanufacturing of terpenoids and offering a reference for the high-value utilization of methanol in synthetic biology.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1866106","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:46:57Z","timestamp":1781848017000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic engineering of Pichia pastoris for sustainable production of 1,8-cineole from methanol"],"prefix":"10.3389","volume":"17","author":[{"given":"Cui","family":"Zhao","sequence":"first","affiliation":[{"name":"Food Nutrition and Health Research Laboratory, School of Health and Elderly Care, Shandong Women\u2019s University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)","place":["Jinan, China"]},{"name":"Key Laboratory of Shandong Microbial Engineering, College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongling","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences)","place":["Jinan, China"]},{"name":"Key Laboratory of Shandong Microbial Engineering, College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanpo","family":"Li","sequence":"additional","affiliation":[{"name":"Food Nutrition and Health Research Laboratory, School of Health and Elderly Care, Shandong Women\u2019s University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Food Nutrition and Health Research Laboratory, School of Health and Elderly Care, Shandong Women\u2019s University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiHui","family":"Wang","sequence":"additional","affiliation":[{"name":"Food Nutrition and Health Research Laboratory, School of Health and Elderly Care, Shandong Women\u2019s University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"115920","DOI":"10.1016\/j.bbr.2025.115920","article-title":"Alpha-pinene attenuates neuroinflammatory responses in the rat hippocampus and improves spatial working memory deficits associated with morphine dependence and withdrawal.","volume":"498","author":"Ahmadi","year":"2026","journal-title":"Behav. 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