{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:40:21Z","timestamp":1789749621810,"version":"4.0.1"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"\n Background<\/jats:title>\n Infection remains a leading cause of morbidity and mortality in burn patients, particularly in those with extensive injuries. However, comprehensive analyses integrating pathogen distribution, antimicrobial resistance, and advanced molecular diagnostics across burn severity remain limited.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We retrospectively analyzed 814 burn patients with infections admitted to two tertiary burn centers in southern China between 2020 and 2024, stratified by total body surface area (TBSA). Pathogen distribution, antimicrobial resistance patterns, complications, hospitalization costs, and length of stay were evaluated. In addition, 40 clinical specimens were prospectively analyzed using both conventional culture and targeted next-generation sequencing (t-NGS).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Among 814 infected burn patients stratified by TBSA (&lt;10%,\n n<\/jats:italic>\n \u202f=\u202f358; 10\u201349%,\n n<\/jats:italic>\n \u202f=\u202f292; \u226550%,\n n<\/jats:italic>\n \u202f=\u202f164), polymicrobial infections rose from 25.7 to 64.4% (\n p<\/jats:italic>\n \u202f&lt;\u202f0.001), Gram-negative bacteria from 27.1 to 56.6% (\n p\u202f&lt;<\/jats:italic>\n 0.001; e.g.,\n Acinetobacter baumannii<\/jats:italic>\n 14.2%,\n Klebsiella pneumoniae<\/jats:italic>\n 18.9%), and fungi from 9.0 to 20.6% (\n p<\/jats:italic>\n \u202f&lt;\u202f0.001; e.g.,\n Candida albicans<\/jats:italic>\n 5.2%). MDR organisms increased, with\n A. baumannii<\/jats:italic>\n &gt;85% resistant to cephalosporins\/carbapenems. Severe burns were associated with higher complication rates (shock 70.7%, MODS 29.3%;\n p<\/jats:italic>\n \u202f&lt;\u202f0.001), 58-day median LOS, and hospitalization costs of 554,246 CNY (\n p<\/jats:italic>\n \u202f&lt;\u202f0.001). In the 40-specimen pilot diagnostic comparison, t-NGS showed 77.5% positivity and culture showed 55.0% positivity (\n p<\/jats:italic>\n \u202f=\u202f0.012), with a shorter turnaround time for t-NGS than culture (1.7 vs. 3.9\u202fdays;\n p<\/jats:italic>\n \u202f&lt;\u202f0.001). In blood specimens, t-NGS positivity was 55.6% compared with 16.7% for conventional culture (\n p<\/jats:italic>\n \u202f=\u202f0.039).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Burn severity is strongly associated with pathogen spectrum and antimicrobial resistance, underscoring the need for severity-stratified management strategies in burn care. In this pilot comparison, t-NGS showed a higher positivity rate than conventional culture and may serve as an adjunct diagnostic method. However, its clinical interpretation must be integrated with phenotypic data given the potential for colonization or genotypic-phenotypic discordance.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1866146","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:03:35Z","timestamp":1784095415000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Epidemiology, pathogen spectrum, and antimicrobial resistance of infections in burn patients stratified by total body surface area: a bicenter study with evaluation of targeted next-generation sequencing"],"prefix":"10.3389","volume":"17","author":[{"given":"Zihao","family":"Ouyang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Department of Clinical Laboratory, Guangzhou Red Cross Hospital of Jinan University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Research Center for Interdisciplinary & High-Quality Innovative Development in Laboratory Medicine","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Surveillance of Bacterial Resistance","place":["Shaoguan, China"]},{"name":"Shaoguan Engineering Research Center for Research and Development of Molecular and Cellular Technology in Rapid Diagnosis of Infectious Diseases and Cancer","place":["Shaoguan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Research Center for Interdisciplinary & High-Quality Innovative Development in Laboratory Medicine","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Surveillance of Bacterial Resistance","place":["Shaoguan, China"]},{"name":"Shaoguan Engineering Research Center for Research and Development of Molecular and Cellular Technology in Rapid Diagnosis of Infectious Diseases and Cancer","place":["Shaoguan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanrong","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Guangzhou Red Cross Hospital of Jinan University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingsen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Research Center for Interdisciplinary & High-Quality Innovative Development in Laboratory Medicine","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Laboratory Medicine, Yuebei People's Hospital Affiliated to Shantou University Medical College","place":["Shaoguan, China"]},{"name":"Shaoguan Municipal Quality Control Center for Surveillance of Bacterial Resistance","place":["Shaoguan, China"]},{"name":"Shaoguan Engineering Research Center for Research and Development of Molecular and Cellular Technology in Rapid Diagnosis of Infectious Diseases and Cancer","place":["Shaoguan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"14364","DOI":"10.1038\/s41598-023-40198-2","article-title":"Epidemiology and mortality in patients hospitalized for burns in Catalonia, Spain","volume":"13","author":"Abarca","year":"2023","journal-title":"Sci. Rep."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"25","DOI":"10.5505\/tjtes.2017.82342","article-title":"A retrospective analysis of 2713 hospitalized burn patients in a burns Centre in Turkey","volume":"24","author":"Albayrak","year":"2017","journal-title":"Ulus. Travma Acil Cerrahi Derg."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3390\/ebj6040053","article-title":"Managing infections in burn patients: strategies and considerations for antimicrobial dosing","volume":"6","author":"Alharthi","year":"2025","journal-title":"Eur. Burn J."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.burnso.2022.03.003","article-title":"Perineal burns: epidemiology, severity factors, management issues and outcome","volume":"6","author":"Baba","year":"2022","journal-title":"Burns Open"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1097\/io9.0000000000000035","article-title":"Length of hospital stay and its predictors among burn patients in a resource limited setting: a multicenter prospective longitudinal study","volume":"62","author":"Baraka","year":"2024","journal-title":"Int. J. Surg. Open"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"S218","DOI":"10.1097\/BCR.0000000000000080","article-title":"Synopsis of the 2013 annual report of the national burn repository","volume":"35","author":"Bessey","year":"2014","journal-title":"J. Burn Care Res."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"R188","DOI":"10.1186\/cc9300","article-title":"Severe burn injury in Europe: a systematic review of the incidence, etiology, morbidity, and mortality","volume":"14","author":"Brusselaers","year":"2010","journal-title":"Crit. Care"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"489","DOI":"10.3760\/cma.j.cn501225-20230827-00067","article-title":"Research advances in the influence of wound external microenvironment factors on wound healing","volume":"40","author":"Cai","year":"2024","journal-title":"Chin J. Burns Wounds"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1093\/jbcr\/irad125","article-title":"American burn association clinical practice guidelines on burn shock resuscitation","volume":"45","author":"Cartotto","year":"2024","journal-title":"J. Burn Care Res."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s44259-025-00169-1","article-title":"Antimicrobial resistance databases: opportunities and challenges for public health","volume":"4","author":"Centner","year":"2026","journal-title":"npj Antimicrob. Resist."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"6991","DOI":"10.2147\/IDR.S547686","article-title":"Comparative evaluation of mNGS and traditional culture methods in pathogen detection for pulmonary infections","volume":"18","author":"Chen","year":"2025","journal-title":"Infect. Drug Resist."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1555307","DOI":"10.3389\/fcimb.2025.1555307","article-title":"Assessment of S100A8\/A9 and resistin as predictive biomarkers for mortality in critically ill patients with sepsis","volume":"15","author":"Chen","year":"2025","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1660347","DOI":"10.3389\/fcimb.2025.1660347","article-title":"Nanopore targeted sequencing in lower respiratory infections: a retrospective study on diagnostic applications, clinical characterization, and antimicrobial guidance","volume":"15","author":"Chen","year":"2025","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1128\/CMR.19.2.403-434.2006","article-title":"Burn wound infections","volume":"19","author":"Church","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/1757-7241-17-14","article-title":"Management of burn injuries \u2013 recent developments in resuscitation, infection control and outcomes research","volume":"17","author":"Dries","year":"2009","journal-title":"Scand. J. Trauma Resusc. Emerg. Med."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1093\/jbcr\/irae075","article-title":"Multidrug-resistant organisms: the silent plight of burn patients","volume":"45","author":"Elsheikh","year":"2024","journal-title":"J. Burn Care Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"10458","DOI":"10.3390\/ijms251910458","article-title":"Gram-negative bacilli blood stream infection in patients with severe burns: microbiological and clinical evidence from a 9-year cohort","volume":"25","author":"Fuentes-Gonz\u00e1lez","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1093\/pubmed\/fds102","article-title":"A systematic review of the epidemiology of unintentional burn injuries in South Asia","volume":"35","author":"Golshan","year":"2013","journal-title":"J. Public Health"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"681731","DOI":"10.3389\/fcimb.2021.681731","article-title":"Different infection profiles and antimicrobial resistance patterns between burn ICU and common wards","volume":"11","author":"Gong","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1186\/s12920-024-02044-w","article-title":"Development of a clinical metagenomics workflow for the diagnosis of wound infections","volume":"17","author":"Halford","year":"2024","journal-title":"BMC Med. Genet."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1093\/jbcr\/irad168","article-title":"Understanding the drivers of cost and length of stay in a cohort of 21,875 patients with severe burn","volume":"45","author":"Hauc","year":"2024","journal-title":"J. Burn Care Res."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.surg.2008.07.001","article-title":"Intensive insulin therapy is associated with reduced infectious complications in burn patients","volume":"144","author":"Hemmila","year":"2008","journal-title":"Surgery"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.hlife.2025.11.005","article-title":"Diagnostics for human pathogenic fungal infections: current status and future prospects","volume":"4","author":"Huang","year":"2026","journal-title":"hLife"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2130","DOI":"10.1093\/cid\/cix682","article-title":"Bacterial infections after burn injuries: impact of multidrug resistance","volume":"65","author":"Lachiewicz","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref25","first-page":"41","article-title":"Risk factors for death and prognosis value of revised baux score for burn patients with inhalation injury","volume":"35","author":"Lam","year":"2022","journal-title":"Ann. Burns Fire Disasters"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"116534","DOI":"10.1016\/j.diagmicrobio.2024.116534","article-title":"Comparison of targeted next-generation sequencing and traditional microbial culture in the diagnosis of pulmonary infections","volume":"110","author":"Liu","year":"2024","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"001808","DOI":"10.1099\/jmm.0.001808","article-title":"Improving pulmonary infection diagnosis with metagenomic next-generation sequencing of bronchoalveolar lavage fluid","volume":"73","author":"Long","year":"2024","journal-title":"J. Med. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/S0140-6736(24)02081-6","article-title":"Multidrug-resistant gram-negative bacterial infections","volume":"405","author":"Macesic","year":"2025","journal-title":"Lancet"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2147\/IDR.S559803","article-title":"Prevalence and determinants of multi-drug resistance bacterial infection among burn patients in a tertiary care center in Nepal","volume":"19","author":"Maharjan","year":"2026","journal-title":"Infect. Drug Resist."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"114769","DOI":"10.1016\/j.addr.2023.114769","article-title":"Burn wound infections microbiome and novel approaches using therapeutic microorganisms in burn wound infection control","volume":"196","author":"Maitz","year":"2023","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/S0305-4179(03)00063-9","article-title":"Work-related burn injuries in Ontario, Canada: has anything changed in the last 10 years?","volume":"29","author":"Mandelcorn","year":"2003","journal-title":"Burns"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1038\/s41522-021-00243-2","article-title":"Burns and biofilms: priority pathogens and in vivo models","volume":"7","author":"Maslova","year":"2021","journal-title":"npj Biofilms and Microbiomes"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.burns.2018.09.011","article-title":"Epidemiology of work-related burn injuries presenting to burn centres in Australia and New Zealand","volume":"45","author":"McInnes","year":"2019","journal-title":"Burns"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1097\/BCR.0b013e31818cb046","article-title":"National burn repository 2007 report: a synopsis of the 2007 call for data","volume":"29","author":"Miller","year":"2008","journal-title":"J. Burn Care Res."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"8707245","DOI":"10.1155\/2024\/8707245","article-title":"Healthcare-associated infections\u2019 characteristics among burn patients and risk factors of mortality: a study based on data from a tertiary center in Iran: nosocomial infections among burn patients","volume":"2024","author":"Mozafari","year":"2024","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/j.jhin.2025.05.010","article-title":"Exploring infection risk factors and multi-drug-resistant organisms (MDROs) in burn intensive care units: a multi-Centre case\u2013control study","volume":"162","author":"Nasser","year":"2025","journal-title":"J. Hosp. Infect."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.3390\/life13061357","article-title":"Blood stream infections in burns: a 14-year cohort analysis","volume":"13","author":"Nitsani","year":"2023","journal-title":"Life"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.jhin.2024.01.002","article-title":"An outbreak of multi-drug-resistant acinetobacter baumannii on a burns ICU and its control with multi-faceted containment measures","volume":"146","author":"Obenhuber","year":"2024","journal-title":"J. Hosp. Infect."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"761","DOI":"10.3390\/ijerph18020761","article-title":"Surviving burn injury: drivers of length of hospital stay","volume":"18","author":"Onah","year":"2021","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.bjps.2024.05.034","article-title":"The epidemiology and outcomes of perineal and genital burn injury in low- and middle- income countries: a global burn registry study","volume":"95","author":"Panayi","year":"2024","journal-title":"J. Plast. Reconstr. Aesthet. Surg."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1016\/j.burns.2011.06.005","article-title":"Epidemiology of burns throughout the world. Part I: distribution and risk factors","volume":"37","author":"Peck","year":"2011","journal-title":"Burns"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"S27","DOI":"10.1016\/j.burns.2004.10.004","article-title":"Burn epidemiology in the Brisbane and Queensland area","volume":"31","author":"Pegg","year":"2005","journal-title":"Burns"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1093\/jbcr\/irac190","article-title":"Epidemiological and clinical characteristics of 471 elderly burn patients in China: a burn center-based study","volume":"44","author":"Qu","year":"2023","journal-title":"J. Burn Care Res."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1588","DOI":"10.3390\/antibiotics12111588","article-title":"Traditional cultures versus next generation sequencing for suspected orthopedic infection: experience gained from a reference centre","volume":"12","author":"Rimoldi","year":"2023","journal-title":"Antibiotics"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"299","DOI":"10.3390\/antibiotics15030299","article-title":"Imipenem-induced transcriptional responses of porin, efflux pumps, and carbapenemase genes in clinical carbapenem-resistant acinetobacter baumannii","volume":"15","author":"Rizvanoglu","year":"2026","journal-title":"Antibiotics"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1089\/sur.2020.129","article-title":"Multi-drug\u2013resistant organisms in burn infections","volume":"22","author":"Robben","year":"2021","journal-title":"Surg. Infect."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"24","DOI":"10.4103\/jimr.jimr_56_24","article-title":"Temporal dynamics of the burn wound microbiome and their resistance towards various antibiotics: a hospital-based observational study","volume":"3","author":"Rout","year":"2025","journal-title":"J. Integr. Med. Res."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"214","DOI":"10.4266\/acc.2023.01571","article-title":"Microbial infections in burn patients","volume":"39","author":"Roy","year":"2024","journal-title":"Acute and Critical Care"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"e00688","DOI":"10.1128\/aac.00688-22","article-title":"Multidrug-resistant gram-negative bacteria in burn patients","volume":"66","author":"Ruegsegger","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1007\/s40495-023-00323-1","article-title":"Rapid diagnostics to enhance therapy selection for the treatment of bacterial infections","volume":"9","author":"Ryu","year":"2023","journal-title":"Curr. Pharmacol. Rep."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"100305","DOI":"10.1016\/j.microb.2025.100305","article-title":"An optimised bead beating RNA extraction method for tough-to-lyse gram-positive bacteria","volume":"7","author":"Scanlon","year":"2025","journal-title":"The Microbe"},{"key":"ref52","first-page":"178","article-title":"Acute respiratory distress syndrome in burn patients: incidence and risk factor analysis","volume":"29","author":"Silva","year":"2016","journal-title":"Ann. Burns Fire Disasters"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1097\/00004630-200207000-00004","article-title":"Serious occupational burn injuries treated at a regional burn center","volume":"23","author":"Taylor","year":"2002","journal-title":"J. Burn Care Rehabil."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1097\/bcr.0b013e3181599bc9","article-title":"American burn association consensus conference to define sepsis and infection in burns","volume":"28","author":"Greenhalgh","year":"2007","journal-title":"J. Burn Care Res."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"e00581","DOI":"10.1128\/msystems.00581-23","article-title":"Economic impact of metagenomic next-generation sequencing versus traditional bacterial culture for postoperative central nervous system infections using a decision analysis mode: study protocol for a randomized controlled trial","volume":"8","author":"Tian","year":"2023","journal-title":"mSystems"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1186\/s12879-021-06790-5","article-title":"Application of mNGS to describe the clinical and microbial characteristics of severe burn a tanker explosion at a tertiary medical center: a retrospective study patients following","volume":"21","author":"Wu","year":"2021","journal-title":"BMC Infect. Dis."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"1594226","DOI":"10.3389\/fmicb.2025.1594226","article-title":"Research progress on the drug resistance mechanisms of candida tropicalis and future solutions","volume":"16","author":"Xiong","year":"2025","journal-title":"Front. Microbiol."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1007\/s40121-024-00983-6","article-title":"Distribution of nosocomial pathogens and antimicrobial resistance among patients with burn injuries in China: a comprehensive research synopsis and meta-analysis","volume":"13","author":"Yang","year":"2024","journal-title":"Infect. Dis. Ther."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"e32423","DOI":"10.1097\/MD.0000000000032423","article-title":"Comparison of next-generation sequencing with traditional methods for pathogen detection in cases of lower respiratory tract infection at a community hospital in eastern China","volume":"101","author":"Yang","year":"2022","journal-title":"Medicine"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"e74848","DOI":"10.7759\/cureus.74848","article-title":"Epidemiological profile of bacterial infections in burn patients over a five-year period","volume":"16","author":"Youssouf","year":"2024","journal-title":"Cureus"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1052293","DOI":"10.3389\/fpubh.2022.1052293","article-title":"Cost analysis of severe burn victims in Southwest China: a 7-year retrospective study","volume":"10","author":"Zeng","year":"2023","journal-title":"Front. Public Health"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"538","DOI":"10.3760\/cma.j.cn501120-20210201-00044","article-title":"Epidemiological investigation and analysis of etiological characteristics of infection on 3067 hospitalized pediatric patients with burns","volume":"37","author":"Zhang","year":"2021","journal-title":"Chin. J. Burns"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"9460","DOI":"10.1038\/s41598-023-36681-5","article-title":"Comparison of pathogen detection consistency between metagenomic next-generation sequencing and blood culture in patients with suspected bloodstream infection","volume":"13","author":"Zhou","year":"2023","journal-title":"Sci. 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