{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:49:48Z","timestamp":1782452988878,"version":"3.54.5"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20241104"],"award-info":[{"award-number":["BK20241104"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019155","name":"Nanjing Drum Tower Hospital","doi-asserted-by":"crossref","award":["2024-LCYJ-PY-34"],"award-info":[{"award-number":["2024-LCYJ-PY-34"]}],"id":[{"id":"10.13039\/501100019155","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Nanjing Health Technology Development Special Fund","award":["ZKX22023"],"award-info":[{"award-number":["ZKX22023"]}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Organ\/space surgical site infection (SSI) is a common and severe complication following biliary-pancreatic surgery. Rapid and accurate diagnosis is crucial for managing organ\/space SSI. The clinical application of targeted next-generation sequencing (tNGS) in biliary-pancreatic surgery remains limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study enrolled 73 consecutive patients who underwent biliary-pancreatic surgery between January 2025 and June 2025, among whom 27 patients were clinically diagnosed with organ\/space SSI. Abdominal drainage specimens were collected and analyzed using tNGS and conventional microbiological tests (CMTs). The diagnostic performance, pathogen spectrum, and turnaround time of the two methods were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall pathogen detection rate was higher for tNGS than for CMTs (52.1% vs. 39.7%). tNGS showed higher sensitivity (92.6% vs. 66.7%) and negative predictive value (94.3% vs. 79.5%) than CMTs in organ\/space SSI, with a shorter turnaround time (28.1\u202fh vs. 109.1\u202fh). tNGS revealed a broader pathogenic spectrum than CMTs (23 vs. 12 species). Moreover, advanced age (OR\u202f=\u202f1.052, 95%CI\u202f=\u202f1.005\u20131.100\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.029) and preoperative biliary drainage (PBD) (OR\u202f=\u202f9.411, 95%CI\u202f=\u202f1.060\u201383.524,\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.044) were associated with tNGS positivity in exploratory multivariate analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>tNGS may provide rapid adjunctive microbiological information for early etiological assessment of organ\/space SSI after biliary-pancreatic surgery, but it should complement rather than replace CMTs and clinical assessment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1866221","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:44:51Z","timestamp":1782452691000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical application of targeted next-generation sequencing in detecting organ\/space surgical site infection in patients following biliary-pancreatic surgery"],"prefix":"10.3389","volume":"17","author":[{"given":"Yifei","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Jiangsu Health Vocational College","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neng","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yudong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1016\/j.surg.2016.11.014","article-title":"The 2016 update of the international study group (ISGPS) definition and grading of postoperative pancreatic fistula: 11 years after","volume":"161","author":"Bassi","year":"2017","journal-title":"Surgery"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1177\/1457496917731188","article-title":"The occurrence of Enterococcus faecium and faecalis is significantly associated with anastomotic leakage after pancreaticoduodenectomy","volume":"107","author":"Belmouhand","year":"2018","journal-title":"Scand. 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