{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:21Z","timestamp":1788419781793,"version":"build-2803163510"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Tibet orbivirus (TIBOV) is a poorly characterized orbivirus whose antigenic diversity and circulation in livestock remain incompletely defined. Here, we report the isolation of a TIBOV strain, MS18C217-16, from\n                    <jats:italic>Culicoides<\/jats:italic>\n                    spp. collected in Mangshi, Yunnan Province, China, in 2018. MS18C217-16 replicated efficiently in multiple mammalian cell lines and induced clear cytopathic effects. Phylogenetic analysis assigned the isolate to the TIBOV clade, with high amino acid conservation in VP1, VP3, and VP7, but marked divergence in the outer-capsid proteins VP2 and VP5. Structural modeling and predicted MHC class II-binding peptide profiles suggested distinctive antigenic features in these proteins, and neutralization assays provided evidence that MS18C217-16 is antigenically distinct from the reference TIBOV strain DH13C120. Serological surveys detected neutralizing antibodies in goats, swine, and cattle across four counties, with particularly high seropositivity in goats from Mengla. Together, these data identify MS18C217-16 as an antigenically divergent TIBOV strain with features consistent with a putative novel serotype, while highlighting the need for reciprocal cross-neutralization assays using strain-specific antisera for formal serotype confirmation.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1867318","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:43:16Z","timestamp":1788417796000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic, antigenic, and seroepidemiological characterization of an antigenically divergent Tibet orbivirus strain isolated from Culicoides in China"],"prefix":"10.3389","volume":"17","author":[{"given":"Yiling","family":"Yang","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Control and Novel Drug Development (Under Construction), School of Public Health, Kunming Medical University","place":["Kunming, China"]},{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Coconstruction by Ministry and Province), Yunnan Animal Science and Veterinary Institute","place":["Kunming, China"]},{"name":"Department of Medical Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yantao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Control and Novel Drug Development (Under Construction), School of Public Health, Kunming Medical University","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"He","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Coconstruction by Ministry and Province), Yunnan Animal Science and Veterinary Institute","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Control and Novel Drug Development (Under Construction), School of Public Health, Kunming Medical University","place":["Kunming, China"]},{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Coconstruction by Ministry and Province), Yunnan Animal Science and Veterinary Institute","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Mangshi Animal Disease Prevention and Control Center","place":["Mangshi, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Coconstruction by Ministry and Province), Yunnan Animal Science and Veterinary Institute","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiju","family":"Chen","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Control and Novel Drug Development (Under Construction), School of Public Health, Kunming Medical University","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Coconstruction by Ministry and Province), Yunnan Animal Science and Veterinary Institute","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Provincial Research Center for Bioinformatic Engineering and Technique, School of Life Sciences and Medicine, Shandong University of Technology","place":["Zibo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangcheng County Animal Disease Prevention and Control Center","place":["Jiangcheng, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University","place":["Beijing, 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