{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:27:21Z","timestamp":1787120841845,"version":"build-2736575974"},"reference-count":87,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009232","name":"University of Debrecen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009232","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Strains of\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      carrying hypervirulence and carbapenemase genes represent a rapidly emerging global public health threat. Our study aimed to comprehensively characterise the genomics of hypervirulence-associated and carbapenemase genes carrying\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      (hv(a)CpKp) isolates in Hungary.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      Between January 2022 and April 2024, 89 aerobactin (\n                      <jats:italic>iucA-D<\/jats:italic>\n                      \/\n                      <jats:italic>iutA<\/jats:italic>\n                      )-positive non-duplicate carbapenemase-producing\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      isolates from 15 Hungarian healthcare institutes underwent short-read (Illumina, MiSeq, NextSeq) whole-genome sequencing, followed by detailed plasmid analysis using long-read sequencing (Nanopore, MinION) in a representative subset of 32 strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Most isolates (79\/89) belonged to the high-risk clone ST147. Hypervirulence-associated (hva) genes\u2014including\n                      <jats:italic>rmpA\/rmpA2, peg344, shiF, iucA\u2013D<\/jats:italic>\n                      , and\n                      <jats:italic>iutA<\/jats:italic>\n                      \u2014were universally present, and 59 isolates possessed chromosomally integrated yersiniabactin loci. Most isolates (87\/89) carried the\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      carbapenemase gene. Hypervirulence-associated genes were most frequently (29\/32) associated with IncHI1B\/IncFIB(Mar) plasmids. Notably, we identified plasmids carrying both hva and carbapenemase genes\u2014designated as hybrid plasmids\u2014in 13 of 32 strains. The\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      was linked to the\n                      <jats:italic>IS26<\/jats:italic>\n                      transposase and was present in conserved, identical cassettes on all\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      -carrying plasmids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion\/conclusion<\/jats:title>\n                    <jats:p>Our study identified hv(a)CpKp strains, particularly the ST147 clone, circulating in Hungary. Our findings highlight the need for routine virulence gene monitoring and continuous genomic and plasmid-based surveillance to mitigate the clinical and epidemiological impact of emerging hv(a)CpKp lineages.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1870558","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:45:28Z","timestamp":1787118328000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Coexistence of carbapenemase and hypervirulence-associated genes among Klebsiella pneumoniae high-risk clones in Hungary"],"prefix":"10.3389","volume":"17","author":[{"given":"Lilla","family":"Buzg\u00f3","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy","place":["Budapest, Hungary"]},{"name":"Department of Infection Control and Hospital Epidemiology, One Health 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of Debrecen","place":["Debrecen, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Mil\u00f3","sequence":"additional","affiliation":[{"name":"Centre for Metagenomics, University of Debrecen","place":["Debrecen, Hungary"]},{"name":"Department of Socio-Geography and Regional Development, Institute of Earth Sciences, Faculty of Science and Technology, University of Debrecen","place":["Debrecen, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Holub","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienn","family":"Hanczvikkel","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Ungv\u00e1ri","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Majoros","sequence":"additional","affiliation":[{"name":"University of Debrecen Clinical Centre, Faculty of Medicine, Institute of Medical Microbiology","place":["Debrecen, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Kamotsay","sequence":"additional","affiliation":[{"name":"South Pest Central Hospital National Institute of Hematology and Infectology, Microbiology Laboratory","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Papp","sequence":"additional","affiliation":[{"name":"Szabolcs-Szatm\u00e1r-Bereg County Hospital and University Teaching Hospital, Central Laboratory","place":["Ny\u00edregyh\u00e1za, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Krist\u00f3f","sequence":"additional","affiliation":[{"name":"Semmelweis University, Faculty of Medicine, Laboratory Medicine Institute","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor","family":"Kardos","sequence":"additional","affiliation":[{"name":"Centre for Metagenomics, University of Debrecen","place":["Debrecen, Hungary"]},{"name":"Department of Planetary Health, One Health Institute, Faculty of Health Sciences, University of Debrecen","place":["Debrecen, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1kos","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy","place":["Budapest, 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