{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:17:11Z","timestamp":1783577831735,"version":"3.55.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Garden asparagus (\n                    <jats:italic>Asparagus officinalis<\/jats:italic>\n                    L.) is an economically important vegetable crop widely cultivated for its nutritional value and health-promoting properties. Asparagus stem blight, caused by\n                    <jats:italic>Phomopsis asparagi<\/jats:italic>\n                    (Sacc.) Bub\u00e1k, is one of the most destructive diseases affecting asparagus production worldwide, leading to significant yield losses. In this study, a pathogenic strain of\n                    <jats:italic>P. asparagi<\/jats:italic>\n                    was isolated and characterized, serving as the target for screening antagonistic microorganisms from the rhizosphere soil of healthy asparagus plants. Among 79 isolates, one strain, identified as\n                    <jats:italic>Bacillus amyloliquefaciens<\/jats:italic>\n                    HN2-9 based on morphological, physiological, and molecular analyses, exhibited the strongest inhibitory activity against the pathogen. HN2-9 caused hyphal deformation, fragmentation, and lysis of\n                    <jats:italic>P. asparagi<\/jats:italic>\n                    . Optimal growth and fermentation conditions were established, achieving a maximum hyphal inhibition rate of 92.1% and spore germination inhibition of 54.8%. In greenhouse assays, HN2-9 significantly reduced disease severity on asparagus stems, with a disease index decrease of 72.4% at OD\u2086\u2080\u2080\u202f=\u202f2, without affecting seed germination or plant growth. These results demonstrate that\n                    <jats:italic>B. amyloliquefaciens<\/jats:italic>\n                    HN2-9 is an effective biocontrol agent against asparagus stem blight and provide a foundation for its development as a sustainable, environmentally friendly disease management strategy.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1872041","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:36:39Z","timestamp":1783575399000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and biocontrol potential of Bacillus amyloliquefaciens against asparagus stem blight"],"prefix":"10.3389","volume":"17","author":[{"given":"Ke-Li","family":"Jia","sequence":"first","affiliation":[{"name":"School of Medical Laboratory, North Henan Medical University","place":["Xinxiang, Henan, China"]},{"name":"College of Life Sciences, Henan Normal University","place":["Xinxiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Fa","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory, North Henan Medical University","place":["Xinxiang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Dong","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory, North Henan Medical University","place":["Xinxiang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu-Jun","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory, North Henan Medical University","place":["Xinxiang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Fen","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory, North Henan Medical University","place":["Xinxiang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1007\/s41348-024-00866-5","article-title":"Evaluation of Bacillus spp. as biocontrol agents against chili leaf spot caused by Xanthomonas vesicatoria","volume":"131","author":"Aziz","year":"2024","journal-title":"J. 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Plant Pathol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"581","DOI":"10.3390\/antibiotics12030581","article-title":"Bacillus VOCs in the context of biological control","volume":"12","author":"Grahovac","year":"2023","journal-title":"Antibiotics"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"208","DOI":"10.36253\/ahsc-18459","article-title":"Stem blight resistance of Asparagus kiusianus and its hybrid with A. officinalis","volume":"28","author":"Iwato","year":"2014","journal-title":"Adv. Hort. Sci."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1007\/s00253-008-1760-3","article-title":"Bacterial volatiles and their action potential","volume":"81","author":"Kai","year":"2009","journal-title":"Appl. Microbiol. 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Biosci."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"105111","DOI":"10.1016\/j.biocontrol.2022.105111","article-title":"Multifaceted Bacillus spp. for the management of cotton bacterial blight caused by Xanthomonas citri pv. Malvacearum","volume":"177","author":"Sampathkumar","year":"2023","journal-title":"Biol. Control"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1094\/pdis-05-19-1040-re","article-title":"Evaluating the sensitivities and efficacies of fungicides with different modes of action against Phomopsis asparagi","volume":"104","author":"Shi","year":"2020","journal-title":"Plant Dis."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/s42483-024-00252-x","article-title":"Cytological and ultrastructural investigation of pathogen infection pathway and host responses in asparagus stem infected by Phomopsis asparagi","volume":"6","author":"Sun","year":"2024","journal-title":"Phytopathol. 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Sci."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s12600-015-0499-5","article-title":"Infection process and pathogenic mechanism of Phomopsis asparagi, the asparagus stem blight pathogen","volume":"44","author":"Yang","year":"2016","journal-title":"Phytoparasitica"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2898","DOI":"10.1094\/PDIS-07-19-1484-RE","article-title":"Biological characteristics and genetic diversity of Phomopsis asparagi, causal agent of asparagus stem blight","volume":"104","author":"Yang","year":"2020","journal-title":"Plant Dis."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"17","DOI":"10.5197\/j.2044-0588.2017.035.017","article-title":"First report of stem blight of asparagus caused by Phomopsis asparagi in Myanmar","volume":"35","author":"Zaw","year":"2017","journal-title":"New Dis. 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