{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:30:06Z","timestamp":1788993006950,"version":"build-2803163510"},"reference-count":87,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The economic feasibility of microalgal biodiesel remains constrained by the high cost of synthetic nutrients, driving interest in waste-derived cultivation systems. This study investigates the mixotrophic growth of\n                    <jats:italic>Desmodesmus<\/jats:italic>\n                    sp. using 100% undiluted matured compost leachate as a dual-purpose medium for biomass production and wastewater remediation. To decouple microalgal assimilation from bacterial degradation, a strictly sterile abiotic control was employed, enabling accurate attribution of nutrient removal pathways. The system achieved bioremediation efficiencies of 82.6% chemical oxygen demand (COD) removal, alongside 60.3% nitrate reduction (via biological assimilation) and 65.5% phosphate reduction (via synergistic biological uptake and photosynthesis-induced physicochemical precipitation).\n                    <jats:italic>Desmodesmus<\/jats:italic>\n                    sp. demonstrated acclimatization to the untreated organic effluent, reaching a maximum biomass concentration of 2.69 g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2014approximately fourfold higher than that obtained in the synthetic control medium. Progressive phosphorus exhaustion, driven by an extreme initial N:P ratio, induced lipid accumulation during the stationary phase, resulting in a lipid yield of 0.715 g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    (26.56% dry cell weight). Fatty acid methyl ester (FAME) analysis revealed a predominantly saturated profile (75.6%), dominated by palmitic and lauric acids, indicating a definitive metabolic shift from the highly unsaturated profile of baseline synthetic media. While such composition enhances oxidative stability, it may impose limitations on cold-flow properties. The detection of a non-saponifiable lipid fraction highlights a process bottleneck that must be addressed during downstream transesterification. This study demonstrates a zero-dilution, waste-to-resource platform that integrates high-density biomass production with nutrient recovery, offering a scalable pathway toward circular bioeconomy-driven biodiesel production.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1872485","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:03:17Z","timestamp":1787724197000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Sustainable bioremediation of undiluted matured compost leachate and enhanced lipid partitioning in Desmodesmus sp.: a zero-dilution approach for circular bioeconomy"],"prefix":"10.3389","volume":"17","author":[{"given":"Charith Akalanka","family":"Dodangodage","sequence":"first","affiliation":[{"name":"ProGreen Lab, Department of Civil Engineering, University of Moratuwa","place":["Moratuwa, Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagath C.","family":"Kasturiarachchi","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences, Sri Lanka Institute of Information Technology","place":["Malabe, Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilini A.","family":"Perera","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of Colombo","place":["Colombo, Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjitha Dilan","family":"Rajapakshe","sequence":"additional","affiliation":[{"name":"Department of Biosystems Technology, Uva Wellassa University","place":["Badulla, Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayuri S.","family":"Niyangoda","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas","place":["Lawrence, KS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rangika Umesh","family":"Halwatura","sequence":"additional","affiliation":[{"name":"ProGreen Lab, Department of Civil Engineering, University of Moratuwa","place":["Moratuwa, Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"100205","DOI":"10.1016\/J.ESE.2022.100205","article-title":"Microalgae-based wastewater treatment: Mechanisms, challenges, recent advances, and future prospects.","volume":"13","author":"Abdelfattah","year":"2023","journal-title":"Environ. 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