{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:28:08Z","timestamp":1787120888809,"version":"build-2736575974"},"reference-count":69,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Anaerobic fermentation (AF) emerged as a promising technology to produce short-chain fatty acids (SCFAs). However, downstream processing of SCFAs is often challenging and costly due to the complexity of AF effluents that present high solids content. Sequencing batch reactors (SBRs) promote effluent separation in two phases (solids and supernatant), which can be an advantage to facilitate SCFAs downstream processing. This work evaluated the performance of SBRs operational configuration at different hydraulic retention times (HRT) (20, 15 and 10 d). The maximum SCFAs concentration of 28.3 g\/L was reached at HRT of 20 d, whereas the maximum bioconversion (60.7%) and productivity (1.8 g SCFAs \/Ld) were reached at HRT of 10 d. Regardless of the HRT, the predominant SCFAs found at the steady state were butyric and acetic acids (&amp;gt; 70% w\/w), in accordance with the detection of acetate and butyrate kinases in all cell lysates. The SBR configuration enabled a 1.3-fold increase in the SCFAs yield (g SCFAs\/g TS\n                    <jats:sub>effluent<\/jats:sub>\n                    ) when comparing the supernatant and the solids phase. The effect of different HRT was analyzed to establish the correlation between SCFAs production yield and the different microbial populations developed in the reactors. Despite the different HRT,\n                    <jats:italic>Clostridium<\/jats:italic>\n                    ,\n                    <jats:italic>Atopobium<\/jats:italic>\n                    , and\n                    <jats:italic>Bulleidia<\/jats:italic>\n                    were the predominant genera in all cases, demonstrating their key role in SCFAs production. These results shed light on the HRT effect to promote high SCFAs accumulation and bioconversion efficiencies under SBR configuration, while easing SCFAs recovery and improving waste revalorization.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1873229","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:48:53Z","timestamp":1787118533000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Anaerobic sequencing batch reactor enhances short-chain fatty acids productivity while facilitating downstream processing"],"prefix":"10.3389","volume":"17","author":[{"given":"Marta","family":"de Vicente","sequence":"first","affiliation":[{"name":"Biotechnological Processes Unit, IMDEA Energy","place":["M\u00f3stoles, Spain"]},{"name":"Faculty of Biological Sciences, Universidad Complutense de Madrid","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Romero","sequence":"additional","affiliation":[{"name":"Biotechnological Processes Unit, IMDEA Energy","place":["M\u00f3stoles, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia","family":"Tom\u00e1s-Pej\u00f3","sequence":"additional","affiliation":[{"name":"Biotechnological Processes Unit, IMDEA Energy","place":["M\u00f3stoles, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Gonzalez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Biotechnological Processes Unit, IMDEA Energy","place":["M\u00f3stoles, Spain"]},{"name":"Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid","place":["Valladolid, Spain"]},{"name":"Institute of Sustainable Processes","place":["Valladolid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"6635","DOI":"10.3390\/molecules28186635","article-title":"Hydraulic retention time as an operational tool for the production of short-chain carboxylates via anaerobic fermentation of carbohydrate-rich waste.","volume":"28","author":"Aboudi","year":"2023","journal-title":"Molecules"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2458369","DOI":"10.1080\/21655979.2025.2458369","article-title":"Assessing the effect of temperature drop on a stable anaerobic fermentation for volatile fatty acids production.","volume":"16","author":"Afecto Gon\u00e7alves","year":"2025","journal-title":"Bioengineered"},{"key":"B3","doi-asserted-by":"publisher","first-page":"2796","DOI":"10.1021\/acs.est.5b04847","article-title":"Chain elongation with reactor microbiomes: Open-culture biotechnology to produce biochemicals.","volume":"50","author":"Angenent","year":"2016","journal-title":"Environ. 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