{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T06:52:59Z","timestamp":1781679179402,"version":"3.54.5"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Potato virus S (PVS) causes severe damage to Solanaceae plants, especially potato, due to its widespread infection and the reliance on laboratory-based reverse transcription-polymerase chain reaction (RT-PCR) testing. This work intends to develop a rapid, highly sensitive, and dual-signal platform for on-site PVS detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed a highly sensitive lateral-flow immunoassay (LFIA) capable of colorimetric and fluorescent dual-mode detection based on a novel core-shell structured probe, which consists of a porous metal-organic framework UiO-66 encapsulating rhodamine B inside and loading gold nanoparticles (AuNPs) outside with 3-aminopropyltriethoxysilane (APTES) as a spacer and glue, denoted as UR@APTES@AuNPs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The developed LFIA enabled dual-mode PVS detection within 15 min with negligible cross-interference from Potato virus X and Potato virus Y. The platform achieved a limit of detection (LOD) of 31.2 pg\/mL for the colorimetric mode and 7.6 pg\/mL for the fluorescent mode, over one order of magnitude lower than the conventional AuNPs-based LFIA (LOD = 462.4 pg\/mL). Furthermore, PVS in field-collected potato leaves was successfully detected by the new LFIA platform.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The dual-mode LFIA demonstrated a rapid, cost-effective, and highly sensitive on-site screening of PVS in field samples, highlighting its great potential in practical application. This platform can be easily adapted to the detection of other pathogenic viruses by using the corresponding specific antibodies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1876426","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T05:56:54Z","timestamp":1781675814000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Rhodamine B\/gold nanoparticles-coloaded UiO-66 as a novel probe for highly sensitive and dual-mode detection of Potato virus S in field samples"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiangting","family":"Li","sequence":"first","affiliation":[{"name":"School of Materials and Energy, Southwest University","place":["Chongqing, China"]},{"name":"School of Tourism & Urban-Rural Planning, Xichang University","place":["Xichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Ren","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Environmental Risks and Related Diseases Precision Control, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhang","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingli","family":"Lei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Ma","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Environmental Risks and Related Diseases Precision Control, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingshuai","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Materials and Energy, Southwest University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"147098","DOI":"10.1016\/j.foodchem.2025.147098","article-title":"Integrating machine learning with lateral flow immunoassay for ultrafast and sensitive tadalafil detection","volume":"498","author":"Chen","year":"2026","journal-title":"Food Chem."},{"key":"B2","doi-asserted-by":"publisher","first-page":"140544","DOI":"10.1016\/j.cej.2022.140544","article-title":"Cefazolin encapsulated UIO-66-NH2 nanoparticles enhance the antibacterial activity and biofilm inhibition against drug-resistant S. aureus: in vitro and in vivo studies","volume":"455","author":"Dastneshan","year":"2023","journal-title":"Chem. 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