{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T06:00:03Z","timestamp":1785218403155,"version":"3.55.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Ulcerative colitis is a chronic inflammatory disease affecting the colon, and its incidence is rising worldwide. Significant alterations to the composition and function of the gut microbiota that favor the expansion of pathobionts have been shown to be involved in ulcerative colitis. One such pathobiont is\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      strains carrying the\n                      <jats:italic>pks<\/jats:italic>\n                      island (\n                      <jats:italic>pks<\/jats:italic>\n                      +\n                      <jats:italic>E. coli<\/jats:italic>\n                      ) that contribute to mucosal injury and exacerbate inflammation. In this study, we investigated whether microbiota-targeting interventions may restrict\n                      <jats:italic>pks<\/jats:italic>\n                      +\n                      <jats:italic>E. coli<\/jats:italic>\n                      expansion and mitigate chemically induced colitis in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In mice colonized with the murine\n                      <jats:italic>pks<\/jats:italic>\n                      +\n                      <jats:italic>E. coli<\/jats:italic>\n                      NC101 strain (EcNC101) in which acute colitis was induced by dextran sulfate sodium, oral administration of putrescine significantly reduced disease severity, histological damage, and inflammatory cytokine expression. 16S rRNA gene sequencing revealed marked shifts in gut microbiota composition following putrescine treatment, characterized by an increase in the relative abundance of Actinobacteriota and a decrease of Proteobacteria phyla. Among these,\n                      <jats:italic>Bifidobacterium pseudolongum<\/jats:italic>\n                      was associated with higher colonic acetate levels and reduced colitis severity. In EcNC101-colonized mice with colitis,\n                      <jats:italic>B. pseudolongum<\/jats:italic>\n                      administration significantly alleviated colitis by increasing acetate levels and activating peroxisome proliferator-activated receptor \u03b3 (PPAR-\u03b3). Functional assays suggested that acetate production by\n                      <jats:italic>B. pseudolongum<\/jats:italic>\n                      contributes to competitive inhibition of EcNC101 growth.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study highlights the potential of microbiota-modifying interventions, particularly probiotics such as\n                      <jats:italic>B. pseudolongum<\/jats:italic>\n                      , to control pathobionts, reduce inflammation, and improve intestinal health in ulcerative colitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1877186","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:34:03Z","timestamp":1785216843000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Expansion resistance against genotoxic E. coli: protective effects of Bifidobacterium pseudolongum in DSS-induced colitis in mice"],"prefix":"10.3389","volume":"17","author":[{"given":"Ayodeji Samuel","family":"Ajayi","sequence":"first","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Fragoso","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Calv\u00e9","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Gerkins","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibault","family":"Maumy","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"McCartney","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela M.","family":"Santos","sequence":"additional","affiliation":[{"name":"Nutrition and Microbiome Laboratory, Institut du cancer de Montr\u00e9al, Centre de recherche du Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)","place":["Montr\u00e9al, QC, Canada"]},{"name":"Department of Medicine, Faculty of Medicine, Universit\u00e9 de Montr\u00e9al","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1136\/gut.2005.081745","article-title":"Peroxisome proliferator activated receptor gamma in colonic epithelial cells protects against experimental inflammatory bowel disease","volume":"55","author":"Adachi","year":"2006","journal-title":"Gut"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1614466","DOI":"10.3389\/fimmu.2025.1614466","article-title":"Hemopexin and HO-1 induction during acute colitis in mice is dependent on interleukin-22","volume":"16","author":"Ajayi","year":"2025","journal-title":"Front. 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