{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:16:52Z","timestamp":1783577812469,"version":"3.55.0"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Hepatitis E virus (HEV) is an emerging zoonotic pathogen of global public health concern. The consumption of raw or undercooked pork products has been identified as a key route of transmission. In this study, the prevalence of HEV RNA was evaluated in commercially available processed pork products in Spain. A total of 280 samples were collected from retail stores and analyzed using a validated RT-qPCR. HEV RNA was detected in 5 samples, corresponding to an overall prevalence of 1.8% (95% CI: 0.6%\u20134.1%). All positive samples were identified in the first sampling quarter, with a significantly higher prevalence (8.9%,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f=\u202f0.001), while no detections were observed in subsequent quarters. No statistically significant associations were found between HEV detection and the presence of preservatives or product type. Among positive samples, viral loads were low, with a median of 12.8 copies\/g and Ct values indicative of low-level contamination. HEV RNA was detected in several product categories, including chorizo, fuet, salchich\u00f3n, and other processed meats. Overall, the results indicate that HEV RNA was detected at low prevalence and low viral loads in processed pork products, suggesting low-level molecular contamination. Although infectivity was not assessed, these findings remain relevant for molecular surveillance and may indicate a potential route of exposure through pork products.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1878664","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:36:29Z","timestamp":1783575389000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of hepatitis E virus RNA in processed pork products: a one-year prevalence study in Spain"],"prefix":"10.3389","volume":"17","author":[{"given":"Mariana Alves","family":"Elois","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]},{"name":"Laboratory of Applied Virology, Department of Microbiology, Immunology, and Parasitology, Federal University of Santa Catarina","place":["Florian\u00f3polis, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Dorighello","family":"Cadamuro","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]},{"name":"Laboratory of Applied Virology, Department of Microbiology, Immunology, and Parasitology, Federal University of Santa Catarina","place":["Florian\u00f3polis, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Yeramian","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Perez-Alonso","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Valero","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, UIC Zoonosis y Enfermedades Emergentes (ENZOEM), University of Cordoba","place":["Cordoba, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca Salguero","family":"Campomar","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique Borges","family":"da Silva Grisard","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Virology, Department of Microbiology, Immunology, and Parasitology, Federal University of Santa Catarina","place":["Florian\u00f3polis, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro Ca\u00f1ete","family":"Reyes","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gislaine","family":"Fongaro","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Virology, Department of Microbiology, Immunology, and Parasitology, Federal University of Santa Catarina","place":["Florian\u00f3polis, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rodr\u00edguez-L\u00e1zaro","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biotechnology and Food Science, University of Burgos","place":["Burgos, Spain"]},{"name":"Centre for Emerging Pathogens and Global Health, University of Burgos","place":["Burgos, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e78","DOI":"10.52225\/NARRA.V2I2.78","article-title":"Hepatitis E virus in pigs and the environment: an updated review of public health concerns","volume":"2","author":"Ahmad","year":"2022","journal-title":"Narra 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