{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:22:14Z","timestamp":1788272534962,"version":"build-2803163510"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fish are among the most frequent causes of immunoglobulin E (IgE)-mediated food allergies (Type I). Currently, there is no known cure for fish allergy and individuals who are sensitized have to practice strict, lifelong avoidance of fish products in their diets. The relationship between gut microbiome and food allergies is currently a major topic of discussion; these pathologies involve the development of dysbiosis, which is a microbial imbalance resulting from immune-related mechanisms. Recent studies have provided evidence that individuals suffering from food allergies, display an intestinal microbiota with a different microbial composition compared to healthy subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives and methods<\/jats:title>\n                    <jats:p>\n                      In this work, we have described for the first time the differences in microbiome composition in a mouse model of fish allergy with previous sensitization to the main allergen, beta-Parvalbumin (\n                      <jats:italic>\u03b2<\/jats:italic>\n                      -PRVB), compared to mouse individuals without allergic response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The metagenomic analysis has shown differences in taxonomic composition between the treatments. Regarding phyla, an increase in the relative abundance of\n                      <jats:italic>Patescibacteria,<\/jats:italic>\n                      specifically\n                      <jats:italic>Saccharimonas<\/jats:italic>\n                      genus and\n                      <jats:italic>Candidatus_Saccharimonas<\/jats:italic>\n                      group, were observed in the allergic animals (Prvb_Alum group) when compared to the other groups. In contrast, the relative abundance of the RF39 group (\n                      <jats:italic>Bacilli<\/jats:italic>\n                      ),\n                      <jats:italic>Atopobiaceae<\/jats:italic>\n                      family,\n                      <jats:italic>Erysipelotrichaceae<\/jats:italic>\n                      , and the\n                      <jats:italic>Coriobacteriaceae<\/jats:italic>\n                      _UCG-002 group, was higher in the animals that did not develop an allergic response, despite being exposed to the allergen (Prvb group). Furthermore, an increase in the relative abundance of\n                      <jats:italic>Lachnospiraceae<\/jats:italic>\n                      ASF356 group was observed in the control group compared to the other treatments. This family, has been reported to be inversely associated with the progression of intestinal inflammation and anaphylactic diseases. For the first time, the gut microbiota composition of individual mice with and without fish allergies is described in detail in this work. This study may shed light on the potential contribution of gut microbiota to the onset and avoidance of food allergies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1886359","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:03:02Z","timestamp":1788271382000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A metagenomic analysis of the gut microbiota in a mouse model of fish allergy"],"prefix":"10.3389","volume":"17","author":[{"given":"Ana G.","family":"Abril","sequence":"first","affiliation":[{"name":"Department of Functional Biology and Health Science, Universidade de Vigo","place":["Vigo, Spain"]},{"name":"Department of Food Technology, Spanish National Research Council (CSIC), Marine Research Institute (IIM-CSIC)","place":["Vigo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Freire","sequence":"additional","affiliation":[{"name":"Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo","place":["Pontevedra, Spain"]},{"name":"Immunology Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO","place":["Pontevedra, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Magad\u00e1n","sequence":"additional","affiliation":[{"name":"Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo","place":["Pontevedra, Spain"]},{"name":"Immunology Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO","place":["Pontevedra, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s G.","family":"Villa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela","place":["Santiago de Compostela, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Pazos","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Spanish National Research Council (CSIC), Marine Research Institute (IIM-CSIC)","place":["Vigo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Carrera","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Spanish National Research Council (CSIC), Marine Research Institute (IIM-CSIC)","place":["Vigo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1038\/s41591-019-0461-z","article-title":"Microbiota therapy acts via a regulatory T cell MyD88\/ROR\u03b3t pathway to suppress food allergy","volume":"25","author":"Abdel-Gadir","year":"2019","journal-title":"Nat. Med."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2234","DOI":"10.3390\/ijms24032234","article-title":"Gut microbiome proteomics in food allergies","volume":"24","author":"Abril","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"ref3","author":"Abril","year":"2022"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1038\/nature12331","article-title":"Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota","volume":"500","author":"Atarashi","year":"2013","journal-title":"Nature"},{"key":"ref9040","doi-asserted-by":"publisher","first-page":"104235","DOI":"10.1016\/j.mvr.2021.104235","article-title":"Electroacupuncture interventions alleviates myocardial ischemia reperfusion injury through regulating gut microbiota in rats","volume":"138","author":"Bai","year":"2021","journal-title":"Microvasc. Res."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref9037","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1099\/00222615-48-9-789","article-title":"Erysipelothrix rhusiopathiae: bacteriology, epidemiology and clinical manifestations of an occupational pathogen","volume":"48","author":"Brooke","year":"1999","journal-title":"J. Med. Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"5688","DOI":"10.1021\/ac200890w","article-title":"Fast monitoring of species-specific peptide biomarkers using high-intensity-focused-ultrasound-assisted tryptic digestion and selected MS\/MS ion monitoring","volume":"83","author":"Carrera","year":"2011","journal-title":"Anal. Chem."},{"key":"ref9004","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S13046-021-02201-4","article-title":"Prevotellaceae produces butyrate to alleviate PD-1\/PD-L1 inhibitor-related cardiotoxicity via PPAR\u03b1-CYP4X1 axis in colonic macrophages","volume":"41","author":"Chen","year":"2022","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.jaci.2017.11.007","article-title":"Food allergy and omics","volume":"141","author":"Dhondalay","year":"2018","journal-title":"J. Allergy Clin. 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Food Chem."},{"key":"ref9030","doi-asserted-by":"publisher","first-page":"3438","DOI":"10.3390\/nu13103438","article-title":"Maturation of the visceral (gut-adipose-liver) network in response to the weaning reaction versus adult age and impact of maternal high-fat diet","volume":"13","author":"Guzzardi","year":"2021","journal-title":"Nutrients"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1073\/pnas.1419038112","article-title":"Cultivation of a human-associated TM7 phylotype reveals a reduced genome and epibiotic parasitic lifestyle","volume":"112","author":"He","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/nmicrobiol.2016.48","article-title":"A new view of the tree of life","volume":"1","author":"Hug","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref9027","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.1111\/1462-2920.12599","article-title":"Modelling the emergent dynamics and major metabolites of the human colonic microbiota","volume":"17","author":"Kettle","year":"2015","journal-title":"Environ. Microbiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"12413","DOI":"10.1073\/pnas.1105695108","article-title":"Identification of markers that distinguish IgE- from IgG-mediated anaphylaxis","volume":"108","author":"Khodoun","year":"2011","journal-title":"Proc. Natl. Acad. Sci. 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Immunol."},{"key":"ref9020","doi-asserted-by":"publisher","first-page":"102339","DOI":"10.1016\/j.anaerobe.2021.102339","article-title":"Bulleidia extructa: an underappreciated anaerobic pathogen","volume":"69","author":"Morgan","year":"2021","journal-title":"Anaerobe"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"201\u2013","DOI":"10.1016\/j.jaci.2012.10.026","article-title":"A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis","volume":"131","author":"Noval Rivas","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref30","first-page":"2825","article-title":"Scikit-learn: machine learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"J. Mach. Learn. Res."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.jaci.2012.11.019","article-title":"Early-life environmental determinants of allergic diseases and the wider pandemic of inflammatory noncommunicable diseases","volume":"131","author":"Prescott","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/1939-4551-6-21","article-title":"A global survey of changing patterns of food allergy burden in children","volume":"6","author":"Prescott","year":"2013","journal-title":"World Allergy Organ. J."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"e9490","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2 - approximately maximum-likelihood trees for large alignments","volume":"5","author":"Price","year":"2010","journal-title":"PLoS One"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"1439452","DOI":"10.3389\/fmicb.2024.1439452","article-title":"Impact of ovalbumin allergy on oral and gut microbiome dynamics in 6-week-old BALB\/c mice","volume":"15","author":"Qiao","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"371","DOI":"10.3389\/fvets.2020.00371","article-title":"Vaginal microbiota changes during estrous cycle in dairy heifers","volume":"7","author":"Quereda","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"13145","DOI":"10.1073\/pnas.1412008111","article-title":"Commensal bacteria protect against food allergen sensitization","volume":"111","author":"Stefka","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"3796","DOI":"10.4049\/jimmunol.145.11.3796","article-title":"IL-4 directs the development of Th2-like helper effectors","volume":"145","author":"Swain","year":"1990","journal-title":"J. Immunol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"e70119","DOI":"10.1111\/IMR.70119","article-title":"Mixed signals: T cells as architects of IgE immunity","volume":"339","author":"Tierney","year":"2026","journal-title":"Immunol. Rev."},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nm.3444","article-title":"Gut microbiota metabolism of dietary fiber influences allergic airway disease and hematopoiesis","volume":"20","author":"Trompette","year":"2014","journal-title":"Nat. Med."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"573","DOI":"10.3390\/microorganisms8040573","article-title":"The controversial role of human gut lachnospiraceae","volume":"8","author":"Vacca","year":"2020","journal-title":"Microorganisms"},{"key":"ref9026","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1186\/s12864-020-06807-4","article-title":"Phylogenomics of expanding uncultured environmental Tenericutes provides insights into their pathogenicity and evolutionary relationship with Bacilli","volume":"21","author":"Wang","year":"2020","journal-title":"BMC Genomics"},{"key":"ref9029","doi-asserted-by":"publisher","first-page":"R473","DOI":"10.1152\/ajpregu.00105.2017","article-title":"High-protein diet improves sensitivity to cholecystokinin and shifts the cecal microbiome without altering brain inflammation in diet-induced obesity in rats","volume":"313","author":"Wang","year":"2017","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref9023","doi-asserted-by":"publisher","first-page":"925","DOI":"10.1002\/ijc.33596","article-title":"Alterations in the oral and gut microbiome of colorectal cancer patients and association with host clinical factors","volume":"149","author":"Wang","year":"2021","journal-title":"Int. J. Cancer"},{"key":"ref9035","doi-asserted-by":"publisher","first-page":"2636336","DOI":"10.1080\/29933935.2026.2636336","article-title":"Strengths and limitations of in vitro and animal models to advance understanding of human diet\u2013microbiome interactions","volume":"3","author":"Weingarden","year":"2026","journal-title":"Gut Microbes Rep."},{"key":"ref90026","doi-asserted-by":"publisher","first-page":"794746","DOI":"10.3389\/fmicb.2021.794746","article-title":"Oral microbiota is associated with immune recovery in human immunodeficiency virus-infected individuals","volume":"12","author":"Xie","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref9002","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1038\/s41392-025-02263-2","article-title":"Microbiota-derived butyrate alleviates asthma via inhibiting Tfh13-mediated IgE production","volume":"10","author":"Yu","year":"2025","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref9025","doi-asserted-by":"publisher","first-page":"668","DOI":"10.26599\/FSHW.2022.9250056","article-title":"Comparison of immune responses and intestinal flora in epicutaneously sensitized BALB\/c or C57BL\/6 mouse models of food allergy","volume":"13","author":"Yu","year":"2024","journal-title":"Food Sci. Human Wellness"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"3315","DOI":"10.3390\/nu13103315","article-title":"Antibiotic-induced gut microbiota dysbiosis damages the intestinal barrier, increasing food allergy in adult mice","volume":"13","author":"Zhang","year":"2021","journal-title":"Nutrients"},{"key":"ref9028","doi-asserted-by":"publisher","first-page":"1684","DOI":"10.3389\/fphar.2019.01684","article-title":"Alpha-glucosidase inhibitors alter gut microbiota and ameliorate collagen-induced arthritis","volume":"10","author":"Zhang","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref9022","doi-asserted-by":"publisher","first-page":"631494","DOI":"10.3389\/fimmu.2021.631494","article-title":"Alteration of intestinal microbiota composition in oral sensitized C3H\/HeJ mice is associated with changes in dendritic cells and T cells in mesenteric lymph nodes","volume":"12","author":"Zhou","year":"2021","journal-title":"Front. 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