{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:28:21Z","timestamp":1786429701819,"version":"build-2736575974"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013129","name":"Ministry of SMEs and Startups","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003653","name":"Korea National Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003653","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation (NRF) of Korea","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Thermophilic species of\n                    <jats:italic>Fervidobacterium<\/jats:italic>\n                    are well known for their ability to degrade native chicken feathers, yet the specific proteases responsible for this activity at the single-enzyme level have remained unresolved because previously identified enzymes primarily acted on synthetic substrates or required synergistic activity in crude extracts. Here, we identified and characterized Fervitase, a feather-degrading thermitase-like S8 serine protease from\n                    <jats:italic>Fervidobacterium islandicum<\/jats:italic>\n                    AW-1. Recombinant Fervitase efficiently hydrolyzed native chicken feathers as a purified enzyme in the presence of dithiothreitol and exhibited maximal activity at 80\u202f\u00b0C and pH 8.0. LC\u2013MS\/MS analysis demonstrated broad cleavage specificity with preferential recognition of threonine, leucine, and serine residues at the P1 position. Notably, Fervitase selectively degraded insoluble feather keratin while exhibiting minimal activity toward soluble protein substrates, suggesting evolutionary specialization for keratin degradation. Structural and mutational analyses further demonstrated that Fervitase-specific structural elements are essential for maturation, catalytic activity and thermostability. Together, this study resolves a long-standing ambiguity in\n                    <jats:italic>Fervidobacterium<\/jats:italic>\n                    keratinolysis and expands current understanding of functional adaptation in thermophilic S8 proteases.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1886669","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T05:40:23Z","timestamp":1786426823000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional identification of a feather-degrading thermitase-like S8 serine protease from Fervidobacterium islandicum AW-1"],"prefix":"10.3389","volume":"17","author":[{"given":"Ji Seob","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Yoon","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon-Su","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakyoo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Graduate Program in Bioindustrial Engineering, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaewon","family":"Nam","sequence":"additional","affiliation":[{"name":"Bio-Living Engineering Major, Global Leaders College, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Yonsei University","place":["Seoul, Republic of Korea"]},{"name":"Graduate Program in Bioindustrial Engineering, Yonsei University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"4930","DOI":"10.1016\/j.biortech.2011.01.042","article-title":"Biodegradability of injection molded bioplastic pots containing polylactic acid and poultry feather fiber","volume":"102","author":"Ahn","year":"2011","journal-title":"Bioresour. 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