{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:04:40Z","timestamp":1784099080581,"version":"3.55.0"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Heavy-metal contamination threatens microbial processes in karst agricultural soils, but functional responses to the bioavailable metal fraction remain insufficiently resolved. We integrated soil properties, total and DTPA-extractable metals, microbial abundance and community profiles, and Biolog EcoPlate traits to quantify microbial functional vulnerability in Pb\u2013Zn mining-affected agricultural soils. DTPA-extractable metal pressure was positively associated with functional vulnerability (Spearman\n                    <jats:italic>\u03c1<\/jats:italic>\n                    \u202f=\u202f0.626,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.001) and negatively associated with carbon-use niche breadth (\n                    <jats:italic>\u03c1<\/jats:italic>\n                    \u202f=\u202f\u22120.481,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.001). Contraction occurred across several substrate classes and remained consistent in covariate-adjusted and sensitivity analyses. Community reorganization, independent contaminated-soil datasets, and a targeted qPCR microcosm experiment further connected the field pattern with shifts in microbial community structure and functional marker genes. These findings identify carbon-use niche contraction as an informative functional response to bioavailable metal mixtures and support its use alongside chemical indicators when assessing remediation and sustainable management of contaminated karst soils.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1887715","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:05:54Z","timestamp":1784095554000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioavailable heavy-metal pressure is linked to microbial functional vulnerability and carbon-use niche contraction in karst agricultural soils"],"prefix":"10.3389","volume":"17","author":[{"given":"Wenming","family":"Liu","sequence":"first","affiliation":[{"name":"Endoscopic Center, The First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]},{"name":"Endoscopic Center, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Shen","sequence":"additional","affiliation":[{"name":"Endoscopic Center, The First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]},{"name":"Endoscopic Center, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxu","family":"Lin","sequence":"additional","affiliation":[{"name":"Endoscopic Center, The First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]},{"name":"Endoscopic Center, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Chemistry & Materials Science, Longyan University","place":["Longyan, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Chemistry & Materials Science, Longyan University","place":["Longyan, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Digestive Endoscopy Center, Fuzhou University Affiliated Provincial Hospital","place":["Fuzhou, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xujin","family":"Wei","sequence":"additional","affiliation":[{"name":"Endoscopic Center, The First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]},{"name":"Endoscopic Center, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University","place":["Fuzhou, Fujian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x","article-title":"A new method for non-parametric multivariate analysis of variance","volume":"26","author":"Anderson","year":"2001","journal-title":"Austral. 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