{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:28:00Z","timestamp":1786343280208,"version":"build-2736575974"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The pseudorabies virus, with enhanced virulence, increased transmissibility, and severe co-infections, have re-emerged worldwide, leading to significant economic losses in swine industry. However, the traditional Bartha-K61 vaccine could not provide full protection against the PRV variant strains, thus, an urgent need for the development of novel prophylactic and therapeutic strategies. Here, we proposed a novel antibody construct, PRV single chain fragment variable (PRV-scFv), for PRV antibody therapy. The PRV-scFv genes were constructed by joining the\n                    <jats:italic>PRV-VH<\/jats:italic>\n                    and\n                    <jats:italic>PRV-VL<\/jats:italic>\n                    sequences, isolated from the immunized mice\u2019s spleen, via a flexible (Gly4Ser)3 linker. This construct was stably expressed in CHO-K1 cells cultured in serum-free suspension. Based on these findings, we screened a monoclonal cell line, CHO-K1-PRV-scFv-2\n                    <jats:sup>E8<\/jats:sup>\n                    cell, to robustly express PRV-scFv antibody that effectively inhibited PRV infection\n                    <jats:italic>in vitro<\/jats:italic>\n                    , reduced the viral load\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and improved the survival rate of infected mice, which provided new insights into antibody therapy and veterinary therapeutic strategies development.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1893255","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T05:31:07Z","timestamp":1786339867000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["CHO-K1 cell-derived scFv targeting pseudorabies virus ameliorates multi-organ viremia and improves animal survival in a lethal infection model"],"prefix":"10.3389","volume":"17","author":[{"given":"Yanmei","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University","place":["Lanzhou, China"]},{"name":"Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Province Livestock Embryo Engineering Research Center, Gansu Agricultural University","place":["Lanzhou, China"]},{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu University","place":["Lanzhou, China"]},{"name":"School of Life Sciences and Engineering, Northwest Minzu University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingying","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu University","place":["Lanzhou, China"]},{"name":"School of Life Sciences and Engineering, Northwest Minzu University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University","place":["Lanzhou, China"]},{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University","place":["Lanzhou, China"]},{"name":"Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Province Livestock Embryo Engineering Research Center, Gansu Agricultural University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruofei","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biotechnology and Bioengineering of State Ethnic Biomedical Research Center, Northwest Minzu University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"106760","DOI":"10.1016\/j.intimp.2020.106760","article-title":"An update on antiviral antibody-based biopharmaceuticals","volume":"86","author":"Ahangarzadeh","year":"2020","journal-title":"Int. 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Commun."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"3379","DOI":"10.1016\/j.vaccine.2014.04.035","article-title":"A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China","volume":"32","author":"Wang","year":"2014","journal-title":"Vaccine"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"191","DOI":"10.5604\/12321966.1152063","article-title":"Animal herpesviruses and their zoonotic potential for cross-species infection","volume":"22","author":"Wozniakowski","year":"2015","journal-title":"Ann. Agric. Environ. 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Purif."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1186\/s12885-016-2168-6","article-title":"A novel anti-p21Ras scFv antibody reacting specifically with human tumour cell lines and primary tumour tissues","volume":"16","author":"Yang","year":"2016","journal-title":"BMC Cancer"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.3390\/v14030547","article-title":"A tug of war: pseudorabies virus and host antiviral innate immunity","volume":"14","author":"Ye","year":"2022","journal-title":"Viruses"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"105919","DOI":"10.1016\/j.rvsc.2025.105919","article-title":"High-yield production and preclinical protection assessment of a Chinese Hamster ovary-K1 (CHO-K1) derived PRV gB and gD subunit vaccine against variant pseudorabies virus in pigs","volume":"196","author":"Yin","year":"2025","journal-title":"Res. Vet. 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Virol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"2562","DOI":"10.1111\/tbed.13297","article-title":"Dynamic cerebrospinal fluid analyses of severe pseudorabies encephalitis","volume":"66","author":"Zheng","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.meegid.2018.12.016","article-title":"Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014","volume":"68","author":"Zhou","year":"2019","journal-title":"Infect. Genet. Evol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1893255\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T05:31:08Z","timestamp":1786339868000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1893255\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":38,"alternative-id":["10.3389\/fmicb.2026.1893255"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2026.1893255","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]},"article-number":"1893255"}}