{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:15:47Z","timestamp":1788419747540,"version":"build-2803163510"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Lyophyllum decastes<\/jats:italic>\n                      is an edible mushroom with nutritional and medicinal value, but approaches for accelerating mycelial biomass accumulation during submerged cultivation remain limited.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study evaluated the effects of Acanthopanax senticosus aqueous extract (ASAE) on submerged mycelial growth and examined associated transcriptional responses using time-course RNA sequencing, weighted gene co-expression network analysis, gene set enrichment analysis, and independent RT-qPCR experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the concentrations tested, 50 mg\/L ASAE produced the greatest biomass accumulation at day 7, increasing mycelial dry weight by 22.7% relative to the untreated control, whereas 1,000 mg\/L ASAE inhibited biomass accumulation. However, the treatment effect was no longer statistically significant at day 9, indicating a transient acceleration of biomass accumulation rather than an increase in final biomass yield under the conditions tested. Two-way ANOVA identified significant effects of cultivation time, treatment, and their interaction on mycelial dry weight. WGCNA module eigengenes were further analyzed using models incorporating cultivation time, treatment, and their interaction. MEblue remained significantly associated with all three factors, including their interaction, but was interpreted as a time- and treatment-associated co-expression module rather than a treatment-specific regulatory module. Direction-separated GSEA showed time-resolved enrichment of gene sets related to energy metabolism, RNA processing, protein homeostasis, carbon metabolism, translation, intracellular trafficking, and carbohydrate-associated metabolism. Upregulated GSEA leading-edge genes were intersected with MEblue genes for candidate-gene visualization without performing a second enrichment analysis. Independent RT-qPCR experiments confirmed the expression trends of selected candidates, including\n                      <jats:italic>CHSG<\/jats:italic>\n                      ,\n                      <jats:italic>UAP1<\/jats:italic>\n                      ,\n                      <jats:italic>ALG5<\/jats:italic>\n                      , and\n                      <jats:italic>RPN12<\/jats:italic>\n                      . A homology-based transcript-collapse sensitivity analysis produced qualitatively similar module- and pathway-level patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These findings indicate that ASAE-associated acceleration of\n                      <jats:italic>L. decastes<\/jats:italic>\n                      biomass accumulation is accompanied by time-resolved transcriptional remodeling. The proposed biological interpretation remains hypothesis-generating, and direct physiological, biochemical, protein-level, and genetic validation will be required to establish causality.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1894250","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:44:10Z","timestamp":1788417850000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Transient acceleration of Lyophyllum decastes mycelial biomass accumulation by Acanthopanax senticosus extract"],"prefix":"10.3389","volume":"17","author":[{"given":"Shiyong","family":"Wen","sequence":"first","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjiao","family":"Li","sequence":"additional","affiliation":[{"name":"Dezhou Academy of Agricultural Sciences, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhen","family":"Jian","sequence":"additional","affiliation":[{"name":"Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot","place":["Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunfan","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiping","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Yang","sequence":"additional","affiliation":[{"name":"Dezhou Municipal Hospital, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huisong","family":"Wang","sequence":"additional","affiliation":[{"name":"Belgorod College of Food Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhennan","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou","place":["Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Res"},{"key":"B2","doi-asserted-by":"publisher","first-page":"aac4354","DOI":"10.1126\/science.aac4354","article-title":"In vivo aspects of protein folding and quality control.","volume":"353","author":"Balchin","year":"2016","journal-title":"Science"},{"key":"B3","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1093\/clinchem\/hvaf043","article-title":"MIQE 2.0: Revision of the minimum information for publication of quantitative real-time PCR experiments guidelines.","volume":"71","author":"Bustin","year":"2025","journal-title":"Clin. 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