{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:01:33Z","timestamp":1790146893280,"version":"4.0.1"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Methanogenic archaea harbor diverse enzymes that support their survival in extreme environments and play key roles in global carbon cycling. The C39 peptidase family, known for cleaving peptide substrates, now gains functional expansion through A0A126R2S0, a novel enzyme that shares structural homology with C39 peptidases (RMSD: 2.0\u20132.5\u202f\u00c5) but exhibits glycosidase activity. The enzyme harbors a conserved Q-C-H motif in which the third residue of the canonical catalytic triad is substituted from aspartate to asparagine. Enzymatic assays demonstrate that A0A126R2S0 specifically hydrolyzes \u03b2-D-galactose analogs, such as p-nitrophenyl-\u03b2-D-galactopyranoside (PNPG, Sub 5), with an activity of 16.15\u202f\u00b1\u202f0.71\u202f\u03bcM\/h, which is 7.8-fold higher than that toward \u03b1-configured analogs. Notably, it shows no detectable activity toward double-glycine peptides or archaeal cell wall polypeptides, distinguishing it from canonical C39 peptidases. Truncation experiments confirm that the C-terminal domain alone is sufficient for this glycosidase activity. Molecular docking and site-directed mutagenesis further implicate Loop 3 (residues D314\u2013D324) as a critical determinant of substrate recognition, with the C316-C322 disulfide bond likely stabilizing its conformation. Collectively, these findings expand the functional repertoire of the C39 peptidase superfamily, suggest an evolutionary connection between peptidases and glycosidases, and establish A0A126R2S0 as a candidate biocatalyst for \u03b2-galactoside processing.<\/jats:p>","DOI":"10.3389\/fmicb.2026.1895254","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:18:15Z","timestamp":1790144295000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A C39-like protein from methanogenic archaea: structural insights and functional divergence in \u03b2-galactose analogue degradation"],"prefix":"10.3389","volume":"17","author":[{"given":"Leizhou","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University","place":["Nanchong, Sichuan, China"]},{"name":"Chengdu Institute of Food Inspection","place":["Chengdu, China"]},{"name":"Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural Affairs","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Health Industry Promotion Center in Dongxing District","place":["Neijiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Bai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University","place":["Nanchong, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1038\/ismej.2017.122","article-title":"The growing tree of Archaea: new perspectives on their diversity, evolution and ecology","volume":"11","author":"Adam","year":"2017","journal-title":"ISME J."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2018.02378","article-title":"Inhibition of rumen methanogens by a novel archaeal lytic enzyme displayed on tailored bionanoparticles","volume":"9","author":"Altermann","year":"2018","journal-title":"Front. 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