{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:52:42Z","timestamp":1791215562061,"version":"4.1.0"},"reference-count":71,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Since its first description in 2003, clinical case reports have repeatedly documented that the bacterial species\n                      <jats:italic>Kerstersia gyiorum<\/jats:italic>\n                      is a human pathogen.\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      has been described in the context of wound and middle ear infections, has been found in urine samples, and has even been recognized as a causative agent of septicemia. However, the pathogenic potential of\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      cannot be clearly determined due to the current lack of information about this species. To reveal the clinical significance of\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      , it is therefore essential to first create a clearly defined reference collection of isolates obtained from routine clinical diagnostics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The aim of this study is to determine the most appropriate method for identifying\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      in routine diagnostics. To this end, three widely used routine methods (VITEK 2, MALDI-TOF MS, and 16S rRNA gene sequencing) were employed, and the accuracy of their results for\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      identification was compared. To ensure accurate species identification, dDDH (an NGS-based identification strategy) was used as the reference method. Furthermore, this manuscript provides an overview of all cases reported in the literature to date and discusses the accuracy of the identification results reported in these studies. In addition, genomic studies (pangenome and ANI matrix) were conducted using in silico analyses to investigate the clonal relationships among the isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results show that MALDI-TOF MS is best suited for identifying\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      in routine diagnostics. However, in contrast to most publications, 16S rRNA gene sequencing was not suitable, as it was impossible to distinguish\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      from other members of the Alcaligenaceae family. The VITEK 2 system must also be considered unreliable for the correct identification of\n                      <jats:italic>K. gyiorum<\/jats:italic>\n                      . Additionally, this study identified a candidate for an undescribed species of the genus Kerstersia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>MALDI-TOF MS is well suited for expanding the reference collection directly from routine diagnostics. The results of 16S gene sequencing should be interpreted with caution. Conversely, biochemical methods must be regarded as unreliable. The species\u2019 large core genome suggests a specialized previously unknown specialized ecological niche. Furthermore, two distinct clusters within the reference collection suggest a common origin. Additionally, follow-up studies must determine whether one isolate that cannot be unambiguously assigned to a species represents a previously undescribed member of the genus Kerstersia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1901541","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:03:52Z","timestamp":1791212632000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of three routine methods for the identification of Kerstersia gyiorum from routine diagnostics and review of the literature"],"prefix":"10.3389","volume":"17","author":[{"given":"Lara","family":"Purschwitz","sequence":"first","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyke","family":"Bunk","sequence":"additional","affiliation":[{"name":"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Percy","family":"Schr\u00f6ttner","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology","place":["Dresden, Germany"]},{"name":"Institute for Medical Microbiology and Virology, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mSphere.01202-20","article-title":"Primer, pipelines, parameters: issues in 16S rRNA gene sequencing","volume":"6","author":"Abellan-Schneyder","year":"2021","journal-title":"mSphere"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"3809","DOI":"10.1128\/JCM.02051-12","article-title":"Isolation of Kerstersia gyiorum from a patient with cholesteatomatous chronic otitis media","volume":"50","author":"Almuzara","year":"2012","journal-title":"J. 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