{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:04:34Z","timestamp":1789538674456,"version":"build-2803163510"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                      (\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      ) is a widespread foodborne pathogen that continues to pose a major challenge to public health and the food industry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To explore the genomic diversity of historical isolates, we used whole genome sequencing (WGS) both short- and long-read technologies to obtain high quality genomes of 64 historical\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      isolates collected between 1918 and 1986. These isolates originated from environmental, animal, and human sources obtained from the collection from Professor Seeliger.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The isolates displayed a substantial genetic diversity, representing 15 clonal complexes (CCs), 40 sequence types (STs) and 59 core genome multilocus sequence types (cgMLST). The most frequently identified STs were ST2 (\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f8), ST7 (\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f6) and ST9 (\n                      <jats:italic>n<\/jats:italic>\n                      \u202f=\u202f4). All isolates harbored known virulence genes, while 35 isolates contained mobile genetic elements (MGEs), ranging from 1 to 11 per isolate. Ten isolates also carried a plasmid. Every isolate possessed the intrinsic resistance genes\n                      <jats:italic>fosX<\/jats:italic>\n                      and\n                      <jats:italic>lin<\/jats:italic>\n                      , and none carried acquired antimicrobial resistance genes (ARGs). Accessory genome diversity mainly followed phylogenetic positioning, and rendered limited within, but not between lineage variability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion\/conclusion<\/jats:title>\n                    <jats:p>\n                      WGS revealed high genetic diversity among the Seeliger\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      isolates. Their close relatedness to contemporary isolates underscores the strong adaptability across sources and time, emphasizing the need for better prevention strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1909666","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:30:44Z","timestamp":1789536644000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic characterization of Listeria monocytogenes strains from the Seeliger\u2019s historical \u201cspecial Listeria culture collection\u201d (1918\u20131986)"],"prefix":"10.3389","volume":"17","author":[{"given":"Beatriz","family":"Daza Prieto","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Pietzka","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Listeria, Division for Public Health, Austrian Agency for Health and Food Safety","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes Benjamin","family":"Stielow","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck","place":["Innsbruck, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Lennkh","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Listeria, Division for Public Health, Austrian Agency for Health and Food Safety","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Murer","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Listeria, Division for Public Health, Austrian Agency for Health and Food Safety","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Eisele","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck","place":["Innsbruck, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"M\u00f6senbacher","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"St\u00f6ger","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Hyden","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Halbedel","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hospital Hygiene, Robert Koch Institute","place":["Wernigerode, Germany"]},{"name":"Institute of Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg","place":["Magdeburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Allerberger","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Hof","sequence":"additional","affiliation":[{"name":"MVZ Labor Limbach and Colleagues","place":["Heidelberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Ruppitsch","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck","place":["Innsbruck, Austria"]},{"name":"Faculty of Food Technology, Food Safety, and Ecology, University of Donja Gorica","place":["Podgorica, Montenegro"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e00034-19","DOI":"10.1128\/mmbr.00034-19","article-title":"Cross talk between SigB and PrfA in Listeria monocytogenes facilitates transitions between extra- and intracellular environments","volume":"83","author":"Ahmed","year":"2019","journal-title":"Microbiol. 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