{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T06:23:46Z","timestamp":1787207026666,"version":"build-2736575974"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    We have isolated and characterized a novel bacteriophage termed Jab, with lytic activity against multidrug-resistant clinical isolates of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . Phage Jab was identified from liquid manure by means of metagenome sequencing of a phage community enrichment using an\n                    <jats:italic>E. coli<\/jats:italic>\n                    clinical isolate ECH07 as host. The initial enrichment was composed of four phages, of which phage Jab represented only a minute fraction (less than 1%). Jab isolation strategy comprised a targeted approach using iterative replication rounds while equipping ECH07 with resistance against the numerically dominant phages coupled with a subsequent host switch to\n                    <jats:italic>E. coli<\/jats:italic>\n                    BL21. Whole-genome sequence analysis revealed only a remote evolutionary distance to known phages within the subfamily\n                    <jats:italic>Vequintavirinae<\/jats:italic>\n                    . The dsDNA genome of phage Jab comprises 142,100 bp (GC content 40.09%) and encodes 264 proteins and five transfer RNAs (tRNAs). No lysogeny-associated proteins were detected, suggesting an obligate lytic lifestyle.\n                    <jats:italic>In silico<\/jats:italic>\n                    genome analysis revealed the presence of at least four putative depolymerases. The closest homology of phage Jab is with members of the new genus\n                    <jats:italic>Septuagintavirus<\/jats:italic>\n                    with around 34% nucleotide identity. VIRIDIC and network analyses strongly suggest that phage Jab belongs to a putative novel genus. The host range of phage Jab is likely restricted to\n                    <jats:italic>E. coli<\/jats:italic>\n                    , displaying a moderately narrow host range (i.e., productive lysis in 8 out of 27 isolates tested). Notably, transmission electron microscopy (TEM) revealed the occurrence of conspicuous unique spherical structures attached at the end of the tail fibers when propagated on BL21 but not when propagated on ECH07. Although their function remains enigmatic, the possible role of those structures as a bacterial (vesicle-based) defense mechanism warrants further investigation.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1909709","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:28:02Z","timestamp":1787203682000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-guided isolation and characterization of a novel bacteriophage infecting Escherichia coli reveal a putative new genus"],"prefix":"10.3389","volume":"17","author":[{"given":"Julia","family":"Oberdorfer","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Tesani","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaysa Leite","family":"Tagliaferri","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Maurice","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva Miriam","family":"Buhl","sequence":"additional","affiliation":[{"name":"Electron Microscopy Facility, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Kraft","sequence":"additional","affiliation":[{"name":"Center for Human Genetics and Genomic Medicine, Faculty of Medicine, RWTH Aachen University","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Kr\u00fcttgen","sequence":"additional","affiliation":[{"name":"Laboratory Diagnostic Center, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Horz","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, RWTH Aachen University Hospital","place":["Aachen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e13338","DOI":"10.1111\/cmi.13338","article-title":"The selective advantage of facultative anaerobes relies on their unique ability to cope with changing oxygen levels during infection","volume":"23","author":"Andr\u00e9","year":"2021","journal-title":"Cell. 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