{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T06:27:14Z","timestamp":1786602434987,"version":"build-2736575974"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Phloridzin is a major autotoxin contributing to apple replant disease (ARD) by inducing oxidative stress and disrupting the rhizosphere ecology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study evaluated the potential of\n                      <jats:italic>Ensifer meliloti<\/jats:italic>\n                      y5077 to mitigate these detrimental effects in\n                      <jats:italic>Malus hupehensis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Strain y5077 demonstrated high degradation efficiency, removing 100% of phloridzin\n                      <jats:italic>in vitro<\/jats:italic>\n                      within 48\u202fh and reducing soil phloridzin levels by 68.4% in 60-day pot experiments. Inoculation significantly improved seedling growth under autotoxic stress, with root dry weight increased compared to non-inoculated stressed plants. These growth benefits were linked to a significant increase in endogenous melatonin levels and the restoration of antioxidant enzyme activities to normal basal levels, which effectively suppressed malondialdehyde (MDA) and reactive oxygen species (ROS) accumulation. Furthermore, y5077 inoculation restructured the rhizosphere microbiome, characterized by significant shifts in the relative abundance of specific microbial taxa (e.g., increases in\n                      <jats:italic>Firmicutes<\/jats:italic>\n                      and\n                      <jats:italic>Actinobacteriota<\/jats:italic>\n                      , and decreases in\n                      <jats:italic>Basidiomycota<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These results demonstrate that y5077 acts as a multi-functional bioremediator that integrates toxin degradation with physiological priming and rhizosphere microbial community restructuring, providing a robust and sustainable biological solution for mitigating phloridzin-induced autotoxic stress in apple seedlings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1912880","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T05:33:25Z","timestamp":1786599205000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Rhizobia mitigates autotoxicity in Malus hupehensis via phenolic degradation and enhanced melatonin-antioxidant responses"],"prefix":"10.3389","volume":"17","author":[{"given":"Cun-cui","family":"Kong","sequence":"first","affiliation":[{"name":"College of Horticulture, Ludong University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Comprehensive Technical Service Center of Zibo Customs","place":["Zibo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-min","family":"Xu","sequence":"additional","affiliation":[{"name":"Shandong Provincial Academy of Environmental Sciences Co., Ltd.","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Xia","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Ludong University","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Coastal Zone Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone, Chinese Academy of Sciences","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-gang","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Coastal Zone Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone, Chinese Academy of Sciences","place":["Yantai, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1111\/jpi.12364","article-title":"Melatonin biosynthesis in plants: multiple pathways catalyze tryptophan to melatonin in the cytoplasm or chloroplasts","volume":"61","author":"Back","year":"2016","journal-title":"J. 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