{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:54:01Z","timestamp":1788274441592,"version":"build-2803163510"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Rapid detection of\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                      in food is critical for implementing timely measures to reduce foodborne listeriosis and subsequent fatalities. While multiple\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      loop-mediated isothermal amplification (LAMP) assays targeting the\n                      <jats:italic>hlyA<\/jats:italic>\n                      gene (listeriolysin O) have been reported in the literature, no independent comparative evaluation has been conducted.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this work, we evaluated the performance of 14\n                      <jats:italic>hlyA<\/jats:italic>\n                      -based\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      LAMP assays, using an FDA-developed\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      LAMP assay targeting the same gene for benchmarking. All assays were performed in an enclosed OptiGene Genie II platform using a harmonized protocol (not individually reported\/optimized conditions). The assay sensitivity (limit of detection, LOD) and specificity (inclusivity\/exclusivity) were determined using a ten-fold serially diluted reference\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      culture (ATCC 19115), and a panel of 50\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      strains, 20 other\n                      <jats:italic>Listeria<\/jats:italic>\n                      spp. strains, and 31 non-\n                      <jats:italic>Listeria<\/jats:italic>\n                      strains, respectively. We also evaluated the quantification capabilities of these LAMP assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Discussion<\/jats:title>\n                    <jats:p>\n                      The LODs for these 14 LAMP assays using\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      ATCC 19115 ranged from exceeding 10\n                      <jats:sup>6<\/jats:sup>\n                      cells per reaction (i.e., nondetectable for three of them at the highest concentration tested) to 10\n                      <jats:sup>2<\/jats:sup>\n                      cells per reaction, with the latter including the FDA LAMP assay. The inclusivity ranged from 20 to 90% detection using the initial templates prepared at approximately 10\n                      <jats:sup>0<\/jats:sup>\n                      -10\n                      <jats:sup>2<\/jats:sup>\n                      cells per reaction whereas 100% inclusivity was observed for two selected and the FDA LAMP assays at higher template concentrations. All 14 assays had 100% exclusivity. The quantification capabilities of these assays varied with correlation coefficients ranging from 0.681 to 0.992. This comprehensive evaluation of 14\n                      <jats:italic>hlyA<\/jats:italic>\n                      -based\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      LAMP assays highlights the critical role of assay design in enabling rapid, sensitive, and specific detection of this prominent foodborne pathogen to ensure food safety and protect public health.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1913307","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:32:47Z","timestamp":1787891567000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative evaluation of 14 hlyA-based loop-mediated isothermal amplification assays for the rapid detection of Listeria monocytogenes"],"prefix":"10.3389","volume":"17","author":[{"given":"Leticia","family":"Mallmann","sequence":"first","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beilei","family":"Ge","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration","place":["Laurel, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madelyn","family":"Springer","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly J.","family":"Domesle","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration","place":["Laurel, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"de Vasconcellos","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Office of Applied Microbiology and Technology, Human Foods Program, U.S. Food and Drug Administration","place":["College Park, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Ximenes","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2719","DOI":"10.1017\/S095026881500254X","article-title":"Tracing sources of Listeria contamination in traditional Italian cheese associated with a US outbreak: investigations in Italy","volume":"144","author":"Acciari","year":"2015","journal-title":"Epidemiol. 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