{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:08:13Z","timestamp":1788761293786,"version":"build-2803163510"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Denitrification in alpine wetlands critically regulates regional nitrogen budgets and N\n                    <jats:sub>2<\/jats:sub>\n                    O emissions, and\n                    <jats:italic>nirK<\/jats:italic>\n                    -harboring denitrifiers represent an important functional group involved in nitrite reduction during denitrification. Spatial heterogeneity across distinct plant communities can modulate soil microenvironments, thereby shaping N\n                    <jats:sub>2<\/jats:sub>\n                    O production, consumption, and emission dynamics. Here, using a space-for-time substitution approach, we investigated four successional stages\u2014early (BS), transitional (CR), middle (EN), and mature (LS)\u2014in the alpine wetlands of Qinghai Lake. High-throughput sequencing combined with a null model framework was applied to characterize the coupling between microbial community patterns and their functional potential. Vegetation succession stimulated microhabitat divergence characterized by shifts in total nitrogen (TN), total carbon (TC), and the carbon-to-nitrogen ratio (CNR). This environmental filtering induced a directional microbial succession, culminating in the highest diversity at the LS stage. The CNR emerged as the primary driver accounting for community variance, mediating a microhabitat niche separation that selectively enriched key heterotrophic denitrifiers (such as\n                    <jats:italic>Sinorhizobium<\/jats:italic>\n                    and\n                    <jats:italic>Paracoccus<\/jats:italic>\n                    ). The \u03b2NTI null model revealed that deterministic processes governed community assembly; notably, the underlying assembly rules underwent a fundamental shift as succession advanced, becoming dominated by heterogeneous selection at the LS stage. Co-occurrence networks peaked in complexity at the EN stage but reorganized into a more consolidated, modular, and highly competitive topology at the LS stage. Furthermore, functional profiling indicated that while the BS stage was functionally oriented toward nitrate respiration and nitrogen fixation, the LS stage exhibited a distinct advantage in possessing predicted denitrification-related functional profiles. In summary, vegetation succession reshaped\n                    <jats:italic>nirK<\/jats:italic>\n                    -type denitrifier communities via environmental filtering, steering the assembly mechanisms from stochasticity toward determinism; meanwhile, the \u201cinsurance effect\u201d of the diverse community structure sustained stable functional output amid nutrient fluctuations. This study clarifies that vegetation succession is a core driving force of the nitrite-reducing potential of\n                    <jats:italic>nirK<\/jats:italic>\n                    -type denitrifiers, providing micro-scale mechanistic insights for the restoration of degraded alpine wetlands, vegetation reconstruction, and the maintenance of regional nitrogen homeostasis under changing climates.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1914409","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T05:33:17Z","timestamp":1788759197000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanisms underlying the effects of alpine wetland vegetation succession on the community assembly and functional potential of nirK-type denitrifier"],"prefix":"10.3389","volume":"17","author":[{"given":"Jiao","family":"Tang","sequence":"first","affiliation":[{"name":"Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University","place":["Xining, China"]},{"name":"Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijia","family":"Zhou","sequence":"additional","affiliation":[{"name":"Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University","place":["Xining, China"]},{"name":"Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University","place":["Xining, China"]},{"name":"Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University","place":["Xining, China"]},{"name":"Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelong","family":"Chen","sequence":"additional","affiliation":[{"name":"Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University","place":["Xining, China"]},{"name":"Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. 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