{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:31:04Z","timestamp":1791297064992,"version":"4.1.0"},"reference-count":76,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Sustainable bioproduction requires developing robust microbial chassis with broad metabolic versatility and suitability for industrial applications.\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    sp. Tol 5 is a highly adhesive bacterium capable of utilizing various hydrocarbons, making it a promising chassis candidate for immobilized whole-cell catalysis. In this study, we characterized the carbon metabolism of Tol 5 by reconstructing metabolic pathway maps from its genomic data and analyzing the transcriptomes of cells grown on ethanol, hexadecane, toluene, and phenol. Genomic analysis revealed that Tol 5 has limited capacity for sugar utilization but possesses a wide range of metabolic pathways for alkane and aromatic compounds, including five distinct aromatic degradation routes that expand the known metabolic diversity of the genus\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    . Transcriptome analysis identified the specific pathway genes induced in response to each carbon source. During growth on phenol, alkylbenzene degradation genes were upregulated alongside phenol monooxygenase genes, suggesting possible substrate-dependent cross-regulation between aromatic degradation pathways. Gene disruption experiments indicated that phenol monooxygenase is required for phenol assimilation, whereas toluene dioxygenase may contribute to earlier entry into exponential growth while potentially limiting final biomass accumulation. These findings provide a comprehensive view of the carbon metabolism of Tol 5 and a basis for assessing its potential in bioprocesses using non-sugar carbon sources.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1919423","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:28:34Z","timestamp":1788150514000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of carbon metabolism in a highly adhesive bacterium Acinetobacter sp. Tol 5 capable of assimilating diverse hydrocarbons and aromatic compounds"],"prefix":"10.3389","volume":"17","author":[{"given":"Shori","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University","place":["Nagoya, Aichi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University","place":["Nagoya, Aichi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiko","family":"Hattori","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University","place":["Nagoya, Aichi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shotaro","family":"Yamagishi","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University","place":["Nagoya, Aichi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsutoshi","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University","place":["Nagoya, Aichi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"122062","DOI":"10.1016\/j.biortech.2019.122062","article-title":"Polyhydroxy butyrate production by Acinetobacter junii BP25, Aeromonas hydrophila ATCC 7966, and their co-culture using a feast and famine strategy","volume":"293","author":"Anburajan","year":"2019","journal-title":"Bioresour. 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