{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:29:40Z","timestamp":1787725780453,"version":"build-2784847793"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    L-glufosinate-ammonium (L-GLA), a widely used herbicide, exerts detrimental non-target effects on crops, soil microorganisms, and ecosystems. However, its impacts on soil microbial communities and metabolic functions remain poorly understood. In this study, we applied L-GLA at two concentrations\u2014600\u202fg a.i. hm\n                    <jats:sup>\u22122<\/jats:sup>\n                    (low, L) and 3,000\u202fg a.i. hm\n                    <jats:sup>\u22122<\/jats:sup>\n                    (high, H)\u2014to yellow-brown soil and investigated its effects on microbial community composition, carbon cycle-related metabolic functions, and soil metabolites using integrated metagenomics and soil environmental pseudotargeted metabolomics at 30 and 60\u202fdays post-application. The degradation rate of L-GLA was concentration-dependent, with half-lives of 18.8\u202fdays (L) and 29.7\u202fdays (H). Both doses significantly reduced soil organic matter (SOM) and available potassium (AK) content, and markedly altered microbial community richness, structure, and composition. L-GLA exposure also disrupted the complexity of soil microbial co-occurrence networks and the activities of carbon-cycle-related enzymes. Metabolomic analysis further revealed significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.05) and dose-dependent alterations in the soil metabolite profile. Correlation analysis indicated strong associations between characteristic microbial taxa and differential metabolites. Our findings provided critical insights into how L-GLA influences the soil microecological environment and contributed to a deeper understanding of soil microbial ecology in the context of modern agricultural practices.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1920057","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:02:58Z","timestamp":1787724178000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into the changes of soil microorganism communities and carbon cycle metabolic functions caused by the application of L-glufosinate-ammonium"],"prefix":"10.3389","volume":"17","author":[{"given":"Yuanfeng","family":"Dai","sequence":"first","affiliation":[{"name":"Bijie Tobacco Company","place":["Bijie, China"]},{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]},{"name":"College of Agriculture, Guizhou University","place":["Guiyang, Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hancheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"He","sequence":"additional","affiliation":[{"name":"Bijie Tobacco Company","place":["Bijie, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Cai","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Wu","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaihao","family":"Zou","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Anshun Tobacco Company","place":["Anshun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuti","family":"Cai","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Agriculture, Guizhou University","place":["Guiyang, Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran Haider","family":"Shamsi","sequence":"additional","affiliation":[{"name":"Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1079\/ejhs.2009\/1034235","article-title":"The in vitro effect of the herbicide Basta\u00ae (Glufosinate ammonium) on potential fungal grapevine pathogens","volume":"74","author":"Albrecht","year":"2009","journal-title":"Eur. 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