{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:17:05Z","timestamp":1788239825654,"version":"build-2803163510"},"reference-count":80,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Rapid screening of\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                      in human and animal foods is imperative for improving laboratory efficiency and safeguarding food supply.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We developed a loop-mediated isothermal amplification (LAMP) assay targeting the\n                      <jats:italic>L. monocytogenes hlyA<\/jats:italic>\n                      gene (listeriolysin O) and performed single-laboratory validation in representative human (soft cheese) and animal (raw pet food) food matrices. This study followed the U.S. Food and Drug Administration\u2019s (FDA) Microbiological Method Validation Guidelines and used the FDA\u2019s\n                      <jats:italic>Bacteriological Analytical Manual<\/jats:italic>\n                      (BAM) method as a reference.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The assay produced accurate results (positive and negative) by analyzing the inclusivity and exclusivity panels comprising 50\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      , 20 non-\n                      <jats:italic>L. monocytogenes Listeria<\/jats:italic>\n                      , and 31 non-\n                      <jats:italic>Listeria<\/jats:italic>\n                      , with a detection limit of 100\u202fcells per reaction, when using\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      American Type Culture Collection (ATCC) 19115. Both soft cheese and raw pet food validations yielded satisfactory results, with the relative levels of detection (RLODs) falling within the acceptability limit (1.5) for a paired study. This suggests that LAMP analysis of 48\u202fh enrichments was as sensitive as the BAM culture method in detecting low-level\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      contamination in both matrices, with reliable screening results available at least 3\u202fdays sooner.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      This study demonstrates the potential of this rapid and reliable LAMP assay for routine screening of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      in soft cheese and raw pet food and, upon further validation, additional human and animal foods to ensure product safety and protect public health.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1920965","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:27:36Z","timestamp":1788236856000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a rapid Listeria monocytogenes loop-mediated isothermal amplification assay in soft cheese and raw pet food"],"prefix":"10.3389","volume":"17","author":[{"given":"Beilei","family":"Ge","sequence":"first","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration","place":["Laurel, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly J.","family":"Domesle","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration","place":["Laurel, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Mallmann","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madelyn","family":"Springer","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Deng","sequence":"additional","affiliation":[{"name":"Joint Institute for Food Safety and Applied Nutrition, University of Maryland","place":["College Park, MD, United States"]},{"name":"U.S. Food and Drug Administration Human Foods Program","place":["College Park, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Jin","family":"Kwon","sequence":"additional","affiliation":[{"name":"U.S. Food and Drug Administration Human Foods Program","place":["College Park, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Ximenes","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health, Indiana University","place":["Bloomington, IN, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"U.S. Food and Drug Administration Human Foods Program","place":["College Park, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref1","volume-title":"Ch 17 Microbiological Methods: Subchapter 10 Listeria","author":"Andrews","year":"2023"},{"key":"ref2","year":"2023"},{"key":"ref3","year":"2026"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"454","DOI":"10.5740\/jaoacint.16-0234","article-title":"Evaluation of the 3M molecular detection assay (MDA) 2\u2014Listeria monocytogenes for the detection of Listeria monocytogenes in a variety of foods and select environmental surfaces: collaborative study, first action 2016.08","volume":"100","author":"Bird","year":"2017","journal-title":"J. AOAC Int."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"980","DOI":"10.5740\/jaoacint.15-031","article-title":"Evaluation of 3M molecular detection assay (MDA) Listeria monocytogenes for the detection of Listeria monocytogenes in selected foods and environmental surfaces: collaborative study, first action 2014.07","volume":"98","author":"Bird","year":"2015","journal-title":"J. AOAC Int."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"5383","DOI":"10.4081\/ijfs.2015.5383","article-title":"Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella Enteritidis and Listeria monocytogenes in artificially contaminated ready-to-eat fresh produce","volume":"4","author":"Birmpa","year":"2015","journal-title":"Ital J Food Saf"},{"key":"ref7","author":"Blodgett","year":"2023"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"2625","DOI":"10.1128\/aem.58.8.2625-2632.1992","article-title":"The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction","volume":"58","author":"Bubert","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1089\/fpd.2021.0080","article-title":"Mpl-gene-based loop-mediated isothermal amplification assay for specific and rapid detection of Listeria monocytogenes in various food samples","volume":"19","author":"Busch","year":"2022","journal-title":"Foodborne Pathog. Dis."},{"key":"ref10","year":""},{"key":"ref11","year":""},{"key":"ref12","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.fshw.2017.03.001","article-title":"PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources","volume":"6","author":"Chen","year":"2017","journal-title":"Food Sci. Human Wellness"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1089\/fpd.2010.0820","article-title":"Development and evaluation of a real-time polymerase chain reaction assay targeting iap for the detection of Listeria monocytogenes in select food matrices","volume":"8","author":"Chen","year":"2011","journal-title":"Foodborne Pathog. Dis."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"4008","DOI":"10.1128\/AEM.00354-11","article-title":"Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification","volume":"77","author":"Chen","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1111\/jfs.12236","article-title":"An advanced visual qualitative and Eva green-based quantitative isothermal amplification method to detect Listeria monocytogenes","volume":"36","author":"Cheng","year":"2016","journal-title":"J. Food Saf."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1007\/s10068-014-0064-x","article-title":"Development of a loop-mediated isothermal amplification assay for detecting Listeria monocytogenesprfA in milk","volume":"23","author":"Cho","year":"2014","journal-title":"Food Sci. Biotechnol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"3629","DOI":"10.1128\/iai.57.11.3629-3636.1989","article-title":"Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation","volume":"57","author":"Cossart","year":"1989","journal-title":"Infect. Immun."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.3390\/foods13111635","article-title":"Evaluating commercial loop-mediated isothermal amplification master mixes for enhanced detection of foodborne pathogens","volume":"13","author":"Costa-Ribeiro","year":"2024","journal-title":"Foods"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.ijfoodmicro.2017.10.020","article-title":"Validation of a Salmonella loop-mediated isothermal amplification assay in animal food","volume":"264","author":"Domesle","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref20","year":"2013"},{"key":"ref21","year":"2019"},{"key":"ref22","year":"2008"},{"key":"ref23","volume-title":"21 CFR Part 117: Current Good Manufacturing Practice, Hazard Analysis, and Risk-Based Preventive Controls for Human Food","year":""},{"key":"ref24","volume-title":"21 CFR Part 507: Current Good Manufacturing Practice, Hazard Analysis, and Risk-Based Preventive Controls for Food for Animals","year":""},{"key":"ref25","year":"2019"},{"key":"ref26","year":""},{"key":"ref27","year":""},{"key":"ref28","author":"Ferguson","year":"2026"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"193","DOI":"10.3390\/foods12010193","article-title":"Application of a loop-mediated isothermal amplification (LAMP) assay for the detection of Listeria monocytogenes in cooked ham","volume":"12","author":"Fiore","year":"2023","journal-title":"Foods"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.foodcont.2017.11.006","article-title":"Development and evaluation of loop-mediated isothermal amplification, and recombinase polymerase amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples","volume":"86","author":"Garrido-Maestu","year":"2018","journal-title":"Food Control"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1681069","DOI":"10.3389\/fvets.2025.1681069","article-title":"Development of a novel p72 gene-based loop-mediated isothermal amplification assay for the rapid detection of African swine fever virus in animal feed","volume":"12","author":"Ge","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"562","DOI":"10.3389\/fmicb.2019.00562","article-title":"Multi-laboratory validation of a loop-mediated isothermal amplification method for screening Salmonella in animal food","volume":"10","author":"Ge","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1126\/science.1063447","article-title":"Comparative genomics of Listeria species","volume":"294","author":"Glaser","year":"2001","journal-title":"Science"},{"key":"ref34","author":"Hitchins","year":"2022"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1166\/jctn.2015.3798","article-title":"Novel micro-nanofluidic chip and device for fast identifying pathogenic bacteria","volume":"12","author":"Huang","year":"2015","journal-title":"J. Comput. Theor. Nanosci."},{"key":"ref36","year":"2016"},{"key":"ref37","year":""},{"key":"ref38","year":""},{"key":"ref39","year":"2026"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"237","DOI":"10.4315\/0362-028x-66.2.237","article-title":"Polymerase chain reaction detection of Listeria monocytogenes on frankfurters using oligonucleotide primers targeting the genes encoding internalin AB","volume":"66","author":"Jung","year":"2003","journal-title":"J. Food Prot."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1271\/bbb.70252","article-title":"Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster","volume":"73","author":"Jung","year":"2009","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"4441","DOI":"10.1128\/aem.63.11.4441-4448.1997","article-title":"Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR","volume":"63","author":"Klein","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/s13205-014-0225-x","article-title":"Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes","volume":"5","author":"Kumar","year":"2015","journal-title":"3 Biotech"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"121834","DOI":"10.1016\/j.jchromb.2019.121834","article-title":"Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification","volume":"1139","author":"Ledlod","year":"2020","journal-title":"J. Chromatogr. B Analyt. Technol. Biomed. Life Sci."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.ijfoodmicro.2003.07.004","article-title":"Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes","volume":"91","author":"Liu","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"e12658","DOI":"10.1111\/jfs.12658","article-title":"Reverse transcription loop-mediated isothermal amplification assays allow the rapid detection of Listeria monocytogenes in fresh-cut fruits and vegetables","volume":"39","author":"Ma","year":"2019","journal-title":"J. Food Saf."},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"1913307","DOI":"10.3389\/fmicb.2026.1913307","article-title":"Comparative evaluation of 14 hlyA-based loop-mediated isothermal amplification assays for the rapid detection of Listeria monocytogenes","volume":"17","author":"Mallmann","year":"2026","journal-title":"Front. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol. Cell. Probes"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.fm.2007.07.007","article-title":"Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target","volume":"25","author":"O\u2019Grady","year":"2008","journal-title":"Food Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3390\/bios7040056","article-title":"Simple screening of Listeria monocytogenes based on a fluorescence assay via a laminated lab-on-paper chip","volume":"7","author":"Pisamayarom","year":"2017","journal-title":"Biosensors"},{"key":"ref51","year":"2026"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1099\/13500872-141-9-2053","article-title":"Listeria monocytogenes exists in at least three evolutionary lines: evidence from flagellin, invasive associated protein and listeriolysin O genes","volume":"141","author":"Rasmussen","year":"1995","journal-title":"Microbiology"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"e12637","DOI":"10.1016\/j.heliyon.2022.e12637","article-title":"Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification","volume":"9","author":"Rivas-Macho","year":"2023","journal-title":"Heliyon"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.femsle.2004.02.018","article-title":"Simultaneous quantitative detection of Listeria spp. and Listeria monocytogenes using a duplex real-time PCR-based assay","volume":"233","author":"Rodriguez-Lazaro","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"669","DOI":"10.3201\/eid3104.240913","article-title":"Foodborne illness acquired in the United States-major pathogens, 2019","volume":"31","author":"Scallan Walter","year":"2025","journal-title":"Emerg. Infect. Dis."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s10068-012-0012-6","article-title":"Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification","volume":"21","author":"Shan","year":"2012","journal-title":"Food Sci. Biotechnol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.4315\/0362-028x-66.9.1658","article-title":"A PCR method based on 16s rRNA sequence for simultaneous detection of the genus Listeria and the species Listeria monocytogenes in food products","volume":"66","author":"Somer","year":"2003","journal-title":"J. Food Prot."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1007\/s00284-011-0013-3","article-title":"Rapid and sensitive detection of Listeria monocytogenes by loop-mediated isothermal amplification","volume":"63","author":"Tang","year":"2011","journal-title":"Curr. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"1955","DOI":"10.1007\/s11274-015-1942-y","article-title":"Mining of novel species-specific primers for PCR detection of Listeria monocytogenes based on genomic approach","volume":"31","author":"Tao","year":"2015","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"6890","DOI":"10.4081\/ijfs.2017.6890","article-title":"Evaluation of a loop-mediated isothermal amplification method for the detection of Listeria monocytogenes in dairy food","volume":"6","author":"Tirloni","year":"2017","journal-title":"Ital J Food Saf"},{"key":"ref61","year":"2014"},{"key":"ref62","year":"2024"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"3763","DOI":"10.1007\/s12161-017-0949-4","article-title":"Development of a rapid screening test for Listeria monocytogenes in raw chicken meat using loop-mediated isothermal amplification (LAMP) and lateral flow dipstick (LFD)","volume":"10","author":"Wachiralurpan","year":"2017","journal-title":"Food Anal. Methods"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3389\/fchem.2018.00090","article-title":"Rapid colorimetric assay for detection of Listeria monocytogenes in food samples using lamp formation of DNA concatemers and gold nanoparticle-DNA probe complex","volume":"6","author":"Wachiralurpan","year":"2018","journal-title":"Front. Chem."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1111\/j.1365-2621.2012.03123.x","article-title":"Development of a propidium monoazide treatment combined with loop-mediated isothermal amplification (PMA-LAMP) assay for rapid detection of viable Listeria monocytogenes","volume":"47","author":"Wan","year":"2012","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1111\/j.1745-4565.2011.00333.x","article-title":"A loop-mediated isothermal amplification assay for rapid detection of Listeria monocytogenes","volume":"31","author":"Wang","year":"2011","journal-title":"J. Food Saf."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1111\/j.1745-4565.2009.00196.x","article-title":"Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detecting Listeria monocytogenes in raw milk","volume":"30","author":"Wang","year":"2010","journal-title":"J. Food Saf."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1007\/s11033-011-0757-7","article-title":"Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains","volume":"39","author":"Wang","year":"2012","journal-title":"Mol. Biol. Rep."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.jmoldx.2015.03.002","article-title":"Multiple endonuclease restriction real-time loop-mediated isothermal amplification: a novel analytically rapid, sensitive, multiplex loop-mediated isothermal amplification detection technique","volume":"17","author":"Wang","year":"2015","journal-title":"J. Mol. Diagn."},{"key":"ref70","year":"2018"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"2743","DOI":"10.1128\/aem.59.8.2743-2745.1993","article-title":"Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay","volume":"59","author":"Wiedmann","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"ref72","author":"Wilrich","year":"2024"},{"key":"ref73","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1007\/s00284-014-0661-1","article-title":"Development of double loop-mediated isothermal amplification to detect Listeria monocytogenes in food","volume":"69","author":"Wu","year":"2014","journal-title":"Curr. Microbiol."},{"key":"ref74","first-page":"1143","article-title":"Sensitive and specific detection of foodborne pathogens in pork by a loop-mediated isothermal amplification methodology","volume":"32","author":"Xiong","year":"2016","journal-title":"Acta Med. Mediterr."},{"key":"ref75","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1089\/fpd.2018.2445","article-title":"Loop-mediated isothermal amplification for Salmonella detection in food and feed: current applications and future directions","volume":"15","author":"Yang","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref76","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1111\/lam.12429","article-title":"Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes","volume":"61","author":"Ye","year":"2015","journal-title":"Lett. Appl. Microbiol."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1007\/s42770-024-01353-7","article-title":"Comparison of real-time PCR and nested PCR based on the hlyA gene for the detection of Listeria monocytogenes. Application on cheese samples","volume":"55","author":"Zbrun","year":"2024","journal-title":"Braz. J. Microbiol."},{"key":"ref78","author":"Zhang","year":"2026"},{"key":"ref79","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.4315\/0362-028x-67.7.1507","article-title":"The BAX PCR assay for screening Listeria monocytogenes targets a partial putative gene lmo2234","volume":"67","author":"Zhang","year":"2004","journal-title":"J. Food Prot."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1920965\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:27:37Z","timestamp":1788236857000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1920965\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":80,"alternative-id":["10.3389\/fmicb.2026.1920965"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2026.1920965","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"article-number":"1920965"}}