{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:22Z","timestamp":1788419782211,"version":"build-2803163510"},"reference-count":75,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Swine production is vital for global food security; however, its sustainability is threatened by the overuse of antibiotics, which contributes to the emergence and dissemination of multidrug-resistant pathogens. Probiotics and bacteriocin-producing bacteria are promising strategies to support animal health and reduce antibiotic dependence. In this study,\n                    <jats:italic>Enterococcus lactis<\/jats:italic>\n                    LBM-M3D, isolated from the porcine gastrointestinal tract, was evaluated for\n                    <jats:italic>in vitro<\/jats:italic>\n                    antimicrobial activity, tolerance to simulated gastrointestinal conditions, genomic safety features, and bacteriocinogenic potential. The strain inhibited several Gram-positive swine-associated pathogens, including\n                    <jats:italic>Streptococcus suis<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus hyicus<\/jats:italic>\n                    ,\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    , and\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    . In addition, LBM-M3D remained viable under simulated gastrointestinal stress conditions, including exposure to pH 3.0 and 0.5% bile salts. Whole-genome sequencing identified four bacteriocin-like sequences, corresponding to enterolysin A, mundticin ATO6, enterocin SE-K4, and enterocin P. No acquired antimicrobial resistance genes were detected in plasmid-associated regions, whereas resistance- and virulence-associated determinants were exclusively chromosomally encoded. Although\n                    <jats:italic>E. lactis<\/jats:italic>\n                    is not currently included in the EFSA Qualified Presumption of Safety (QPS) list due to limited species-level safety data, our genomic analyses provide strain-level evidence supporting the safety of LBM-M3D. Overall, these findings indicate that LBM-M3D possesses probiotic-associated characteristics and represents a promising candidate for further probiotic development in swine production, pending additional functional validation, with potential applications in sustainable livestock systems.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1920982","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:41:29Z","timestamp":1788417689000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic safety, bacteriocinogenic potential, and probiotic-associated traits of Enterococcus lactis LBM-M3D isolated from the swine gastrointestinal tract"],"prefix":"10.3389","volume":"17","author":[{"given":"Fernando Moises Mamani","family":"Sanca","sequence":"first","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Emilio Cabrera","family":"Matajira","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Berm\u00fadez-Puga","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u00eds Mayumi","family":"Kuniyoshi","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"de Medeiros Oliveira","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Biotechnology, College of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University (UNESP)","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iago Rodrigues","family":"Blanco","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Camargo","family":"Knirsch","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Sciences, Vigo University, Campus of Ourense","place":["Ourense, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Micke","family":"Moreno","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"de Mello Varani","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Biotechnology, College of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University (UNESP)","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Pinheiro","family":"de Souza Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, S\u00e3o Paulo University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1186\/s12864-023-09749-9","article-title":"Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species","volume":"24","author":"Ahmed","year":"2023","journal-title":"BMC Genomics"},{"key":"ref2","year":"2026"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1128\/jb.177.8.2125-2137.1995","article-title":"The genes involved in production of and immunity to sakacin a, a bacteriocin from Lactobacillus sake Lb706","volume":"177","author":"Axelsson","year":"1995","journal-title":"J. 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