{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:09:37Z","timestamp":1789567777072,"version":"build-2803163510"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Tobacco root rot, caused by\n                      <jats:italic>Fusarium<\/jats:italic>\n                      species, is a persistent soil-borne disease that threatens tobacco production. To identify endophytic contributors to disease suppression, this study compared the root endophytic microbiomes of healthy and diseased tobacco plants using metagenomic sequencing and isolated functional bacteria from healthy roots.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Metagenomic analysis of 30 root samples (223 Gb) generated 2.9 million non-redundant genes and identified 1,953 core genera. Healthy plants contained distinct endophytic microbial communities enriched in bacterial taxa and pathways associated with secondary metabolite biosynthesis, siderophore production, chemotaxis, biofilm formation, and carbohydrate metabolism. This microbiome-guided approach identified TM-1, an endophytic\n                      <jats:italic>Enterobacter<\/jats:italic>\n                      strain that significantly inhibited\n                      <jats:italic>Fusarium solani<\/jats:italic>\n                      by 62.29% in a dual-culture assay. Transcriptome profiling revealed that TM-1 treatment broadly altered\n                      <jats:italic>F. solani<\/jats:italic>\n                      gene expression, with prominent effects on ribosome function, amino acid biosynthesis, carbon metabolism, and glycolysis. TM-1 disrupted sugar transporter-related gene expression, and deletion of five representative genes significantly restricted fungal mycelial growth, with the strongest inhibition (66.47%) observed for the hexose transporter homolog MRS44_010803.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results indicate that healthy tobacco roots harbor disease-suppressive endophytic microorganisms and suggest that TM-1 may suppress\n                      <jats:italic>F. solani<\/jats:italic>\n                      by interfering with sugar transport and carbon acquisition. These findings provide a potential biocontrol resource for the sustainable management of tobacco root rot.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1924993","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:36:28Z","timestamp":1787636188000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic profiling of tobacco root endophytes reveals a disease-suppressive Enterobacter strain against Fusarium solani"],"prefix":"10.3389","volume":"17","author":[{"given":"Tianmiao","family":"Li","sequence":"first","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]},{"name":"State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]},{"name":"State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]},{"name":"State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianfu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Dali Tobacco Company of Yunnan","place":["Dali, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Jiang","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhan","family":"Cai","sequence":"additional","affiliation":[{"name":"Qujing Tobacco Company of Yunnan","place":["Qujing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Hu","sequence":"additional","affiliation":[{"name":"Dali Tobacco Company of Yunnan","place":["Dali, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canhua","family":"Lu","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhu","family":"Xuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Gai","sequence":"additional","affiliation":[{"name":"Yunnan Academy of Tobacco Agricultural Sciences","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/B978-0-12-820526-6.00015-4","volume-title":"New and Future Developments in Microbial Biotechnology and Bioengineering","author":"Al-Ani","year":"2020"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s41938-020-00313-1","article-title":"Fusarium wilt biocontrol and tomato growth stimulation, using endophytic bacteria naturally associated with Solanum sodomaeum and S. bonariense plants","volume":"30","author":"Aydi-Ben-Abdallah","year":"2020","journal-title":"Egypt. 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