{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:59:53Z","timestamp":1790873993598,"version":"4.1.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Hepatocellular carcinoma (HCC) arises in heterogeneous etiological contexts, but direct cross-omics comparisons between HCC subgroups defined by different etiologies remain limited. In this exploratory study, we combined fecal 16S rRNA gene sequencing and untargeted serum metabolomics with exploratory cross-omics analyses to compare gut microbial composition, serum metabolic profiles, and microbiome\u2013metabolite association patterns between hepatitis B virus-related HCC (HBV-HCC) and alcohol-related HCC (ALD-HCC). Forty patients who underwent primary tumor resection were included, comprising 26 with HBV-HCC and 14 with ALD-HCC. Alpha diversity indices did not differ significantly between groups, whereas within-group Bray\u2013Curtis dissimilarity was greater in ALD-HCC and genus-level beta-diversity ordination showed only modest separation between the two groups. LEfSe-based screening suggested the enrichment of candidate taxa, including\n                    <jats:italic>Faecalibacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Roseburia<\/jats:italic>\n                    ,\n                    <jats:italic>Lachnospira<\/jats:italic>\n                    ,\n                    <jats:italic>Turicibacter<\/jats:italic>\n                    , and\n                    <jats:italic>Butyribacter<\/jats:italic>\n                    in HBV-HCC, whereas\n                    <jats:italic>Allisonella<\/jats:italic>\n                    and\n                    <jats:italic>Coprobacillus<\/jats:italic>\n                    were enriched in ALD-HCC. Untargeted metabolomics showed group separation in supervised models and identified exploratory candidate metabolic features mainly involving glycerophospholipid metabolism, primary bile acid biosynthesis, aminoacyl-tRNA biosynthesis, and central carbon metabolism in cancer; however, these metabolite-level findings did not remain significant after multiple-testing correction. Procrustes and O2PLS analyses indicated limited global concordance between the microbiome and metabolome, although exploratory associations were observed between several phosphatidylcholine-related features and microbial taxa. Overall, HBV-HCC and ALD-HCC may be associated with different gut microbial and serum metabolic patterns, and these findings should be considered exploratory and hypothesis-generating.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1927595","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:45:10Z","timestamp":1790743510000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Etiology-associated differences in the gut microbiome and metabolome in hepatocellular carcinoma: an exploratory study of alcohol-related and HBV-related cases"],"prefix":"10.3389","volume":"17","author":[{"given":"Yongguang","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University","place":["Nanning, Guangxi, China"]},{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kejian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinchen","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huasheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University","place":["Nanning, Guangxi, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University","place":["Nanning, Guangxi, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Shang","sequence":"additional","affiliation":[{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Department of Hepatobiliary Gland Surgery, The Ninth Affiliated Hospital of Guangxi Medical University, Beihai People\u2019s Hospita","place":["Beihai, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer (Guangxi Medical University)","place":["Nanning, China"]},{"name":"Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education","place":["Nanning, Guangxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University","place":["Nanning, Guangxi, China"]},{"name":"Guangxi Medical University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1038\/s41467-020-20422-7","article-title":"Gut microbiota impact on the peripheral immune response in non-alcoholic fatty liver disease related hepatocellular carcinoma","volume":"12","author":"Behary","year":"2021","journal-title":"Nat. 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Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1927595\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:08:06Z","timestamp":1790870886000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2026.1927595\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":46,"alternative-id":["10.3389\/fmicb.2026.1927595"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2026.1927595","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"article-number":"1927595"}}