{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:07:15Z","timestamp":1788934035528,"version":"build-2803163510"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    In this study, response surface methodology was used to optimize a sequential two-stage process comprising enzymatic pretreatment followed by simultaneous microwave\u2013ultrasound-assisted extraction of\n                    <jats:italic>Ophiopogon japonicus<\/jats:italic>\n                    polysaccharides. At the unrounded model optimum (208.492\u202fW microwave power, 300.945\u202fW ultrasonic power, 25.207\u202fmin enzymatic hydrolysis, and 30.100\u202fmin microwave\u2013ultrasound extraction), the predicted crude polysaccharide extract yield was 43.999% (44.0% when rounded). Two fractions, OJP-1 and OJP-2, were obtained by DEAE-52 and Sephadex G-100 chromatography. Structural analyses showed differences in their monosaccharide composition, spectral properties, surface morphology, thermal stability, and HPGPC molecular-mass profiles.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , OJP-2 showed stronger radical-scavenging and probiotic growth-promoting responses than OJP-1, whereas both fractions resisted hydrolysis in the simulated digestion systems. OJP-2\u2019s stronger responses were accompanied by distinct compositional, morphological, thermal, and HPGPC molecular-mass-profile characteristics.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1927939","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:49:54Z","timestamp":1788932994000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural characterisation and antioxidant and prebiotic activities of Ophiopogon japonicus polysaccharides obtained by enzymatic pretreatment followed by simultaneous microwave\u2013ultrasound-assisted extraction"],"prefix":"10.3389","volume":"17","author":[{"given":"Henan","family":"Sun","sequence":"first","affiliation":[{"name":"School of Life Sciences, Zhuhai College of Science and Technology","place":["Zhuhai, China"]},{"name":"College of Life Sciences, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Zhuhai College of Science and Technology","place":["Zhuhai, China"]},{"name":"College of Life Sciences, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiang","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Zhuhai College of Science and Technology","place":["Zhuhai, China"]},{"name":"College of Life Sciences, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Sarengaowa","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Zhuhai College of Science and Technology","place":["Zhuhai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Feng","sequence":"additional","affiliation":[{"name":"Medical Sciences Division, Macau University of Science and Technology","place":["Taipa, Macao SAR, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhong","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Zhuhai College of Science and Technology","place":["Zhuhai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"147066","DOI":"10.1016\/j.foodchem.2025.147066","article-title":"Correlation analysis of monosaccharide composition and in vitro antioxidant and hypoglycemic activities of Tremella fuciformis spore polysaccharides","volume":"498","author":"Chen","year":"2026","journal-title":"Food Chem."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.ijbiomac.2017.12.034","article-title":"Preparation, deproteinization, characterisation, and antioxidant activity of polysaccharide from cucumber (Cucumis saticus L.)","volume":"108","author":"Chen","year":"2018","journal-title":"Int. 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