{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T06:04:13Z","timestamp":1789365853513,"version":"build-2803163510"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Aromatic alcohols such as 2-phenylethanol (2-PE) and tryptophol (TOL) possess diverse biological functions. Here, an unfolded protein response-inducible gene\n                    <jats:italic>ULI1<\/jats:italic>\n                    and an aldehyde dehydrogenase gene\n                    <jats:italic>ALD3<\/jats:italic>\n                    in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    were disrupted using the CRISPR-Cas9 system. Fermentation assays revealed that both the\n                    <jats:italic>ULI1<\/jats:italic>\n                    frameshift (Kmuli1) and knockout (K\u0394uli1) mutants exhibited significantly reduced 2-PE and TOL yields relative to the wild-type strain KMLY1-6, retaining 46.32 and 64.54% for 2-PE, and 34.61 and 77.73% for TOL, respectively. In contrast,\n                    <jats:italic>ALD3<\/jats:italic>\n                    knockout moderately elevated 2-PE and TOL production by 4.98 and 20.57%, respectively. These results indicate that both\n                    <jats:italic>ULI1<\/jats:italic>\n                    and\n                    <jats:italic>ALD3<\/jats:italic>\n                    mediate the biosynthesis of 2-PE and TOL in\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    . To further confirm the role of\n                    <jats:italic>ULI1<\/jats:italic>\n                    , three chromosomal integration expression (CIE) strains (KXuli1, KXIIuli1, K\u0394ald3uli1) and one plasmid-based expression (PBE) strain (BY4741\/pY26-uli1) were constructed. Compared with the control KMLY1-6, the CIE strains increased 2-PE and TOL production by 10.13\u201312.91% and 25.76\u201329.28%, respectively. Meanwhile, the PBE strain produced significantly higher levels of 2-PE and TOL than the empty vector control (BY4741\/pY26), with maximum increases of 38.80% and 30.86% detected at 60 h of cultivation. To further elucidate the molecular mechanism of\n                    <jats:italic>ULI1<\/jats:italic>\n                    in 2-PE and TOL biosynthesis, comparative transcriptomic analysis for Kmuli1\n                    <jats:italic>vs.<\/jats:italic>\n                    KMLY1-6, together with transcriptomic and proteomic analyses for BY4741\/pY26-uli1\n                    <jats:italic>vs.<\/jats:italic>\n                    BY4741\/pY26, was performed. Multi-omics results revealed that ULI1 reinforces the Ehrlich pathway, modulates metabolic flux, maintains mitochondrial structure and cellular protein homeostasis, and coordinates stress responses with metabolic redistribution. Collectively, ULI1 acts as a novel regulatory hub linking stress tolerance and cellular homeostasis, and facilitates aromatic alcohol biosynthesis by strengthening the Ehrlich pathway in\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1934502","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:39:17Z","timestamp":1789364357000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["An unfolded protein response-inducible protein (ULI1) mediates aromatic alcohols biosynthesis through reinforcing the Ehrlich pathway in Saccharomyces cerevisiae"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaowei","family":"Gong","sequence":"first","affiliation":[{"name":"R&D Center of China Tobacco Yunnan Industrial Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Shen","sequence":"additional","affiliation":[{"name":"National R&D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanzhai","family":"Shang","sequence":"additional","affiliation":[{"name":"R&D Center of China Tobacco Yunnan Industrial Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Zhan","sequence":"additional","affiliation":[{"name":"R&D Center of China Tobacco Yunnan Industrial Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Ma","sequence":"additional","affiliation":[{"name":"Yunnan Forestry Technological College","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajun","family":"Luo","sequence":"additional","affiliation":[{"name":"Faculty of Life Science and Technology, Kunming University of Science and Technology","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwei","family":"Li","sequence":"additional","affiliation":[{"name":"R&D Center of China Tobacco Yunnan Industrial Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxia","family":"Song","sequence":"additional","affiliation":[{"name":"Faculty of Life Science and Technology, Kunming University of Science and Technology","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donglai","family":"Zhu","sequence":"additional","affiliation":[{"name":"R&D Center of China Tobacco Yunnan Industrial Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyong","family":"Luo","sequence":"additional","affiliation":[{"name":"National R&D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University","place":["Nanchang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.3389\/fmolb.2019.00011","article-title":"Structure and molecular mechanism of ER stress signaling by the unfolded protein response signal activator IRE1.","volume":"6","author":"Adams","year":"2019","journal-title":"Front. 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