{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:07:37Z","timestamp":1788934057436,"version":"build-2803163510"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth Research Fund of Anhui Medical University","award":["2021xkj061"],"award-info":[{"award-number":["2021xkj061"]}]},{"name":"Research Fund of Anhui Institute of Translational Medicine","award":["2024zh-06"],"award-info":[{"award-number":["2024zh-06"]}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Severe fever with thrombocytopenia syndrome virus (SFTSV), a novel bunyavirus associated with febrile illness, thrombocytopenia, and leukopenia, represents a considerable public health threat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we developed a visual detection platform for SFTSV by integrating reverse transcription-recombinase polymerase amplification (RT-RPA) with lateral flow nucleic acid chromatography assay (LFNA). The method operates under isothermal conditions, and delivers results within 40 minutes. Using a conserved region of the S segment as the amplification target.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The assay demonstrated a detection limit of 10\n                      <jats:sup>1<\/jats:sup>\n                      copies\/\u03bcL and showed no cross-reactivity with 12 other common clinical pathogens or 6 less common clinical pathogens. An evaluation of 80 SFTSV-positive clinical samples and 16 SFTSV-negative clinical samples showed that, compared with quantitative PCR (qPCR), the method demonstrated excellent sensitivity and specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The RT-RPA-LFNA platform is easy to operate, provides rapid results and is cost-effective, and therefore has potential for use in point-of-care testing (POCT), particularly in resource-limited primary healthcare settings. This approach not only provides a reliable tool for SFTSV diagnosis but also holds potential for the detection of other pathogens.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1934648","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:58:37Z","timestamp":1788933517000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of a rapid detection method for severe fever with thrombocytopenia syndrome virus based on reverse transcription-recombinase polymerase amplification with lateral flow nucleic acid chromatography assay technology"],"prefix":"10.3389","volume":"17","author":[{"given":"Zhoujie","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongchuan","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lufei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shenzhen Tailored Medical Ltd.","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilu","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]},{"name":"Department of Clinical Laboratory, Anhui Public Health Clinical Center","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2023\/6661723","article-title":"Diversity of viruses in Ixodes ricinus in Europe including novel and potential arboviruses","volume":"2023","author":"Bratuleanu","year":"2023","journal-title":"Transbound. 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